Ronald Phillip Marshall

US Patent:

8580495, Nov 12, 2013

Filed:

Apr 21, 2010

Appl. No.:

12/764301

Inventors:

Edward K. Pabich - Oak Lawn IL, US
Ronald L. Marshall - San Diego CA, US
Hong Yu - Long Grove IL, US

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04

US Classification:

435 61, 435 912, 536 231, 536 243

Abstract:

Polynucleotides useful for detecting and/or in a test sample, kits, a nucleic acid amplification method and detection method including the same.

US Patent:

54279305, Jun 27, 1995

Filed:

Jun 28, 1991

Appl. No.:

7/722798

Inventors:

Larry G. Birkenmeyer - Chicago IL
John J. Carrino - Gurnee IL
Bruce J. Dille - Antioch IL
Hsiang-Yun Hu - Libertyville IL
Jon D. Kratochvil - Kenosha WI
Thomas G. Laffler - Libertyville IL
Ronald L. Marshall - Zion IL
Laurie A. Rinehardt - Kenosha WI
Natalie A. Solomon - Buffalo Grove IL

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

C12P 1934
C12Q 168
C07H 2104

US Classification:

435 9152

Abstract:

An improved, "gap filling" embodiment of the Ligase Chain Reaction (LCR) is described. Gap filling LCR is LCR wherein at least one of the probes is recessed so that a gap is formed between the adjacent probes when they are hybridized to target. The gap is filled using polymerase and deoxyribonucleotide triphosphates before ligation of the probes together. There are single and double gap versions, depending on whether one or two probes are recessed and require filling before ligation. The improvement resides in selecting and using target sequences such that only a single type, or two types, of deoxyribonucleotide triphosphate(s) are required to fill double gaps each being 1-10 bases in length, preferably 1-3 bases. Probes having specific sequences are claimed for a number of pathogens.