Ronald Michael Paul

US Patent:

7625712, Dec 1, 2009

Filed:

May 17, 2005

Appl. No.:

11/130492

Inventors:

Ronald D. Paul - Fort Lauderdale FL, US
James L. Wyatt - San Diego CA, US
Barbara Carrillo - Miami FL, US
Oilda Rubio - Miami FL, US
Diana B. Careaga - Miami FL, US
Lidice L. Lopez - Miami FL, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N 33/567

US Classification:

435 721, 435 723, 435 724, 4352852, 4352871, 4352872, 4352887, 436 10, 436 17, 436 56, 436164, 436171, 436175, 436546, 422 8201, 422 8205, 422 8207

Abstract:

A method useful for the enumeration of cell populations in a biological sample includes the steps of reacting in a single reaction mixture a sample, a first antibody labeled with a fluorochrome having a first emission spectrum and an additional antibody. The first antibody binds to an antigenic determinant differentially expressed on leukocytes and non-leukocytes. The additional antibody binds to an antigenic determinant differentially expressed on mature and immature granulocytes or myeloid cells, and is labeled either with the first fluorochrome or an additional fluorochrome having an emission spectrum distinguishable from the first emission spectrum. The reaction mixture can be mixed with a nucleic acid dye having an emission spectrum that overlaps with one of the first or additional emission spectra. The reaction mixture may be treated with a lytic system that differentially lyses non-nucleated red blood cells and conserves leukocytes. Populations of hematological cells are detected and enumerated using at least two parameters (fluorescence, optical, and electrical) for each population.

US Patent:

7674598, Mar 9, 2010

Filed:

Jun 27, 2006

Appl. No.:

11/475313

Inventors:

Ronald D. Paul - Fort Lauderdale FL, US
Oilda Rubio - Miami FL, US
Diana B. Careaga - Miami FL, US
Lidice L. Lopez - Miami FL, US
Ravindra Mylvaganam - Longmeadow MA, US

Assignee:

Beckman Coulter, Inc. - Fullerton CA

International Classification:

G01N 33/567

US Classification:

435 724, 435 71, 435 721, 435 723, 4352852, 4352871, 4352872, 4352887, 436 10, 436 17, 436 56, 436164, 436171, 436175, 436546, 422 8201, 422 8205, 422 8207

Abstract:

Methods for differentially identifying cells in an instrument employ compositions containing a combination of selected antibodies and fluorescent dyes having different cellular distribution patterns and specificities, as well as antibodies and fluorescent dyes characterized by overlapping emission spectra which form non-compensatable spectral patterns. When utilizing the compositions described herein consisting of fluorescent dyes and fluorochrome labeled antibodies with overlapping spectra that cannot be separated or distinguished based upon optical or electronic compensation means, a new fluorescent footprint is established. This new fluorescent footprint is a result of the overlapping spectra and the combined cellular staining patterns of the dyes and fluorochrome labeled antibodies chosen for the composition. The new fluorescent footprint results in histogram patterns that are useful for the identification of additional cell populations or subtypes in hematological disease.

US Patent:

52388123, Aug 24, 1993

Filed:

May 1, 1990

Appl. No.:

7/517309

Inventors:

Wallace H. Coulter - Miami Springs FL
John D. Hollinger - Miami FL
Thomas Russell - Miami FL
Carlos Rodriguez - Miami FL
Ronald Paul - North Miami Beach FL

Assignee:

Coulter Corporation - Miami FL

International Classification:

C12N 100
G01N 33543

US Classification:

435 72

Abstract:

A method and apparatus for accelerating at least one definitive biological reaction including increasing the accuracy of determinations made therefrom. The reaction involves selected viable biological cells which are prepared in a small sample volume and rapidly mixed with microspheres having antibody specific at least to specific ones of the cells bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed to analyze the remaining blood cell populations. The microspheres can be introduced sequentially or simultaneously.

US Patent:

54647525, Nov 7, 1995

Filed:

Apr 8, 1993

Appl. No.:

8/046404

Inventors:

Kenneth H. Kortright - Cooper City FL
Wallace H. Coulter - Miami Springs FL
Carlos Rodriguez - Miami FL
Thomas Russell - Miami FL
Ronald Paul - North Miami Beach FL

Assignee:

Coulter Corporation - Miami FL

International Classification:

C12Q 124
G01N 33577

US Classification:

435 724

Abstract:

A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected white blood cell population and/or subset thereof of a whole blood sample or portion thereof. A volume of a biological sample containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

US Patent:

62286528, May 8, 2001

Filed:

Feb 16, 1999

Appl. No.:

9/251019

Inventors:

Carlos M. Rodriguez - Miami FL
Jose M. Cano - Miami FL
Barbara Carrillo - Miami FL
Kristie M. Gordon - Coral Gables FL
Allan F. Horton - Miami FL
Ronald D. Paul - Fort Lauderdale FL
Mark A. Wells - Davie FL
James L. Wyatt - Plantation FL

Assignee:

Coulter International Corp. - Miami FL

International Classification:

G01N 3348

US Classification:

436 63

Abstract:

A blood analyzing instrument includes a single transducer for simultaneously measuring the DC volume, RF conductivity, light scattering and fluorescence characteristics of blood cells passing through a cell-interrogation zone. Preferably, the transducer includes an electro-optical flow cell which defines a cell-interrogation zone having a square transverse cross-section measuring approximately 50. times. 50 microns, and having a length, measured in the direction of cell flow, of approximately 65 microns.

US Patent:

54379857, Aug 1, 1995

Filed:

Jul 8, 1991

Appl. No.:

7/726467

Inventors:

Jorge A. Quintana - Miami FL
Thomas R. Russell - Miami FL
Ronald D. Paul - North Miami Beach FL
Timothy J. Fischer - Plantation FL

Assignee:

Coulter Corporation - Miami FL

International Classification:

G01N 3348

US Classification:

435 724

Abstract:

The method of this invention is directed to the rapid preparation of a whole blood sample for photooptical analysis. In the preferred embodiments of this method, a whole blood sample, lytic reagent system and immunological stain (optional) are contacted with the sample in a common reaction vessel (i. e. cuvette or test tube), with gentle asymmetric vortex mixing, so as to maintain the particulate matter of the sample at an essentially homogeneous concentration throughout the sample. An aliquot of the contents of the reaction vessel can, thereafter, be analyzed for identification and/or quantification of the analyte of interest. This process is conservative of the various endogenous constituents of the sample since virtually all of the manipulative steps involved in the preparation of the sample occur within a common vessel and any excess (unconsumed or unreacted) reagents and stain need not be separated from the sample during such preparation, thus, avoiding the multiple wash steps traditionally associated with this process. This process is uniquely applicable to the conservative preparation of aged whole blood samples (samples in which the endogenous nutrients have been essentially completely consumed) and whole blood samples from diseased state patients wherein the disease is manifest by changes in the morphology, total number and/or relative concentration of one or more of the lymphocyte sub-populations (i. e. B and/or T-cells).

US Patent:

52310051, Jul 27, 1993

Filed:

Mar 10, 1992

Appl. No.:

7/849481

Inventors:

Thomas Russell - Miami FL
Kenneth H. Kortright - Davie FL
Wallace H. Coulter - Miami Springs FL
Carlos M. Rodriguez - Miami FL
Ronald Paul - North Miami Beach FL
Constance M. Hajek - Miami FL
James C. Hudson - Miami FL

Assignee:

Coulter Corporation - Hialeah FL

International Classification:

C12Q 100
C12Q 106
C12N 100

US Classification:

435 721

Abstract:

A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population and subset analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

US Patent:

52233987, Jun 29, 1993

Filed:

Sep 20, 1990

Appl. No.:

7/587646

Inventors:

Kenneth H. Kortright - Cooper City FL
Wallace H. Coulter - Miami Springs FL
Carlos Rodriguez - Miami FL
Thomas Russell - Miami FL
Ronald Paul - North Miami Beach FL

Assignee:

Coulter Corporation - Hialeah FL

International Classification:

C12Q 102

US Classification:

435 724

Abstract:

A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected white blood cell population and/or subset thereof of a whole blood sample or portion thereof. A volume of a biological sample containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.