Ryan F Workman

US Patent:

7914999, Mar 29, 2011

Filed:

Dec 29, 2006

Appl. No.:

11/618495

Inventors:

Frank C. Grenier - Libertyville IL, US
Ryan F. Workman - Waukegan IL, US
Hina N. Syed - Gurnee IL, US
Salman Ali - Hoffman Estates IL, US

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 33/53

US Classification:

435 71, 435 86, 436 4

Abstract:

The invention provides a lysis reagent and method for preparing a test sample for use in an assay, wherein the method yields a homogeneous lysis mixture suitable for use in automated pipetting systems without the need for a centrifugation step. The lysis reagent includes a glycol and an alcohol. Other aspects of the invention include related immunoassays and test kits.

US Patent:

8221986, Jul 17, 2012

Filed:

Jun 25, 2009

Appl. No.:

12/491309

Inventors:

Frank C. Grenier - Libertyville IL, US
Ryan F. Workman - Gurnee IL, US
Hina N. Syed - Gurnee IL, US
Salman Ali - Hoffman Estates IL, US

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 33/53

US Classification:

435 71, 436518

Abstract:

The invention provides methods of preparing a test sample for use in an assay for detecting an analyte bound by an intracellular ligand. The methods typically involve contacting the test sample with an assay reagent comprising: a lysis reagent; and a protease that has proteolytic activity for said intracellular ligand; to form a mixture compatible for use in an immunoassay without subsequent extraction steps. Other aspects of the invention include related immunoassays and test kits.

US Patent:

8394606, Mar 12, 2013

Filed:

Nov 4, 2009

Appl. No.:

12/612061

Inventors:

Salman Ali - Hoffman Estates IL, US
Ryan M. Bonn - Kenosha WI, US
Frank C. Grenier - Libertyville IL, US
Tracey D. Rae - Glenview IL, US
Kevin R. Rupprecht - Gurnee IL, US
Hina N. Syed - Gurnee IL, US
Bailin Tu - Libertyville IL, US
Joan D. Tyner - Beach Park IL, US
Ryan F. Workman - Gurnee IL, US

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

C07K 1/14

US Classification:

435 691, 530424

Abstract:

A composition comprising neutrophil gelatinase-associated lipocalin (NGAL), which has been enriched from urine, has a molecular weight of about 24. 9 kDa to about 25. 9 kDa, and comprises a plurality of isoforms having isoelectric points (pIs) ranging from about 5. 9 to about 9. 1; a composition comprising NGAL, which has been enriched from recombinant Chinese hamster ovary (CHO) cells, has a molecular weight of about 25. 9 kDa to about 27. 9 kDa, and comprises a plurality of isoforms having pIs ranging from about 5. 6 to about 9. 1; a method of obtaining from urine a composition comprising a plurality of isoforms of NGAL, which method comprises enriching NGAL in urine without separating molecules based on charge; a method of obtaining from recombinant CHO cells a composition comprising a plurality of isoforms of NGAL, which method comprises enriching NGAL in a composition without separating molecules based on charge; and a method of analyzing NGAL isoforms enriched from urine or recombinant CHO cells comprising analyzing an enriched composition comprising NGAL isoforms by two-dimensional electrophoresis and Western blot.

US Patent:

8440416, May 14, 2013

Filed:

Jul 12, 2012

Appl. No.:

13/547525

Inventors:

Frank C. Grenier - Libertyville IL, US
Ryan F. Workman - Gurnee IL, US
Hina N. Syed - Gurnee IL, US
Salman Ali - Hoffman Estates IL, US

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 33/53

US Classification:

435 71, 436518

Abstract:

The invention provides methods of preparing a test sample for use in an assay for detecting an analyte bound by an intracellular ligand. The methods typically involve contacting the test sample with an assay reagent comprising: a lysis reagent; and a protease that has proteolytic activity for said intracellular ligand; to form a mixture compatible for use in an immunoassay without subsequent extraction steps. Other aspects of the invention include related immunoassays and test kits.

US Patent:

8541554, Sep 24, 2013

Filed:

Dec 29, 2010

Appl. No.:

12/980877

Inventors:

Frank C. Grenier - Libertyville IL, US
Ryan F. Workman - Gurnee IL, US
Hina Syed - Gurnee IL, US
Salman Ali - Hoffman Estates IL, US

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

C07K 16/00
G01N 33/24

US Classification:

5303898, 435 80, 435 81, 530421

Abstract:

An improved extractive reagent composition and method for extracting an immunosuppressant drug, such as sirolimus, tacrolimus or cyclosporine, from blood samples while yielding a test sample extract that has low vapor pressure and is compatible with immunoassay components. The inventive reagent composition comprises dimethyl sulfoxide (DMSO), at least one divalent metal salt and water. The sample extracts resulting from use of each of these combinations have low vapor pressure and are compatible with immunochemistry assays.

US Patent:

20080020401, Jan 24, 2008

Filed:

Jul 21, 2006

Appl. No.:

11/490624

Inventors:

Frank C. Grenier - Libertyville IL, US
Ryan F. Workman - Waukegan IL, US
Hina Syed - Gurnee IL, US
Salman Ali - Hoffman Estates IL, US

International Classification:

G01N 33/53

US Classification:

435 71

Abstract:

An improved extractive reagent composition and method for extracting an immunosuppressant drug, such as sirolimus, tacrolimus or cyclosporine, from blood samples while yielding a test sample extract that has low vapor pressure and is compatible with immunoassay components. The inventive reagent composition comprises dimethyl sulfoxide (DMSO), at least one divalent metal salt and water. The sample extracts resulting from use of each of these combinations have low vapor pressure and are compatible with immunochemistry assays.

US Patent:

20090123946, May 14, 2009

Filed:

Dec 5, 2008

Appl. No.:

12/329331

Inventors:

Larry G. Birkenmeyer - Glenview IL, US
Suresh M. Desai - Libertyville IL, US
Frank C. Grenier - Libertyville IL, US
David J. Hawksworth - Lake Villa IL, US
Edward T. Olejniczak - Grayslake IL, US
Qiaoqiao Ruan - Round Lake IL, US
Robert W. Siegel - Fountaintown IN, US
Sergey Y. Tetin - Lindenhurst IL, US
Bryan C. Tieman - Elmhurst IL, US
Bailin Tu - Libertyville IL, US
Joan D. Tyner - Beach Park IL, US
Ryan F. Workman - Gurnee IL, US
Robert N. Ziemann - Lindenhurst IL, US
Lowell J. Tyner - Chicago IL, US

Assignee:

ABBOTT LABORATORIES - Abbott Park IL

International Classification:

G01N 33/53

US Classification:

435 721

Abstract:

The present invention relates to NGAL immunoassays and kits, and to methods of using glycosylated mammalian NGAL and antibodies that bind to mammalian NGAL in immunoassays and kits. Among other things, the methods and kits can be employed to determine the amount of human NGAL monomer in a test sample, as well as to determine the proportion of human NGAL monomer to human NGAL dimer contained in a test sample.

US Patent:

20090269777, Oct 29, 2009

Filed:

Apr 16, 2008

Appl. No.:

12/104410

Inventors:

Larry G. Birkenmeyer - Glenview IL, US
Suresh M. Desai - Libertyville IL, US
Frank C. Grenier - Libertyville IL, US
David J. Hawksworth - Lake Villa IL, US
Edward T. Olejniczak - Grayslake IL, US
Qiaoqiao Ruan - Round Lake IL, US
Robert W. Siegel - Fountaintown IN, US
Sergey Y. Tetin - Lindenhurst IL, US
Bryan C. Tieman - Elmhurst IL, US
Bailin Tu - Libertyville IL, US
Joan D. Tyner - Beach Park IL, US
Lowell J. Tyner - Chicago IL, US
Ryan F. Workman - Waukegan IL, US
Robert N. Ziemann - Lindenhurst IL, US

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 33/53

US Classification:

435 71

Abstract:

The present invention relates to NGAL immunoassays and kits, and to methods of using glycosylated mammalian NGAL and antibodies that bind to mammalian NGAL in immunoassays and kits. Among other things, the methods and kits can be employed to determine the amount of human NGAL monomer in a test sample, as well as to determine the proportion of human NGAL monomer to human NGAL dimer contained in a test sample.