Steven M. Menchen - Fremont CA Linda G. Lee - Palo Alto CA Charles R. Connell - Redwood City CA N. Davis Hershey - San Carlos CA Vergine Chakerian - San Mateo CA Sam L. Woo - Redwood City CA Steven Fung - Palo Alto CA
Assignee:
The Perkin-Elmer Corporation - Foster City CA
International Classification:
C07D31182
US Classification:
549223, 549224, 549225, 549382, 549388
Abstract:
Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2â,4â,5â,7â-tetrachloro-4,7-dicloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
Steven M. Menchen - Fremont CA Linda G. Lee - Palo Alto CA Charles R. Connell - Redwood City CA N. Davis Hershey - San Carlos CA Vergine Chakerian - San Mateo CA Sam L. Woo - Redwood City CA Steven Fung - Palo Alto CA
Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting specially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2â,4â,5â,7â-tetrachloro-4,7-dichloro-5-(and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
Mobility-Modified Nucleobase Polymers And Methods Of Using Same
Sam L. Woo - Redwood City CA Ron Graham - San Ramon CA Jing Tian - Mountain View CA
Assignee:
Applera Corporation - Foster City CA
International Classification:
C07H 2100
US Classification:
536 231, 435 6, 536 253, 536 266
Abstract:
The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.
Probe Composition Comprising A Binding Polymer And Polymer Chain And Methods Of Use
Paul David Grossman - Hillsborough CA Steven Fung - Palo Alto CA Steven Michael Menchen - Fremont CA Sam Lee Woo - Redwood City CA Emily Susan Winn-Deen - Pleasanton CA
Assignee:
Applera Corporation - Foster City CA
International Classification:
C12Q 168
US Classification:
435 6, 435 911, 435 912
Abstract:
Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.
Probe Composition Containing A Binding Domain And Polymer Chain And Methods Of Use
Paul David Grossman - Hillsborough CA Steven Fung - Palo Alto CA Steven Michael Menchen - Fremont CA Sam Lee Woo - Redwood City CA Emily Susan Winn-Deen - Foster City CA
Assignee:
Applera Corporation - Foster City CA
International Classification:
C12Q 168
US Classification:
435 6, 435 911, 435 912
Abstract:
Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.
Mobility-Modified Nucleobase Polymers And Methods Of Using Same
Sam Lee Woo - Redwood City CA, US Ronald J. Graham - San Ramon CA, US Jing Tian - Mountain View CA, US
Assignee:
Applera Corporation - Foster City CA
International Classification:
C12Q 1/68 C07H 21/00 C07H 21/02
US Classification:
435 6, 536 231, 536 253, 536 266
Abstract:
The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.
Method For Distinguishing Different-Sequence Polynucleotides
Paul David Grossman - Burlingame CA, US Steven Fung - Palo Alto CA, US Steven Michael Menchen - Fremont CA, US Sam Lee Woo - Redwood City CA, US Emily Susan Winn-Deen - Foster City CA, US
Assignee:
Applera Corporation - Foster City CA
International Classification:
C12Q 1/68 C12P 19/34
US Classification:
435 6, 435 911, 435 912
Abstract:
Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.
Method For Distinguishing Different-Sequence Polynucleotides
Paul David Grossman - Burlingame CA, US Steven Fung - Palo Alto CA, US Steven Michael Menchen - Fremont CA, US Sam Lee Woo - Redwood City CA, US Emily Susan Winn-Deen - Foster City CA, US
Assignee:
Applera Coporation - Foster City CA
International Classification:
C12Q 1/68 C12P 19/34
US Classification:
435 6, 435 911, 435 912
Abstract:
Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.
Academic Consulting & Edication
Senior Director and Co-Founder
Aceprep Mar 2012 - Dec 2017
Director of Operations
Twin Creek Elemtary School Mar 2012 - Dec 2017
Gate Students Math Coach
Aceprep Oct 2007 - Dec 2013
Director of Mathematics and Computer Science
Ccb First Academy In Houston Aug 2004 - Oct 2007
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Education:
Rice University 2002 - 2004
Masters, Applied Mathematics
University of Illinois at Urbana - Champaign 2002
Bachelors, Mathematics, Computer Science
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Thermo Fisher Scientific Jan 2015 - Jan 2016
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Nv 2011 - 2014
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Life Technologies Nov 1984 - Apr 2010
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Fbn Nov 1984 - Apr 2010
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Woodrow Wilson High School 1967 - 1970
Portola Jr High School 1964 - 1967
Bayview Elementary School 1957 - 1964
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West Covina, CAManager, Technical Services at Hot Topic Past: Manager, Network Operations at Sport Chalet, Director, Network Operations at Aames Home...
Rollins Elementary School Augusta GA 1967-1971, North Ranchito Elementary School Pico Rivera CA 1971-1972, Abraham Lincoln Elementary School Whittier CA 1972-1973, Dexter Junior High School Whittier CA 1973-1975