Sam Kwong Woo

US Patent:

6403812, Jun 11, 2002

Filed:

May 30, 2000

Appl. No.:

09/580754

Inventors:

Steven M. Menchen - Fremont CA
Linda G. Lee - Palo Alto CA
Charles R. Connell - Redwood City CA
N. Davis Hershey - San Carlos CA
Vergine Chakerian - San Mateo CA
Sam L. Woo - Redwood City CA
Steven Fung - Palo Alto CA

Assignee:

The Perkin-Elmer Corporation - Foster City CA

International Classification:

C07D31182

US Classification:

549223, 549224, 549225, 549382, 549388

Abstract:

Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2â,4â,5â,7â-tetrachloro-4,7-dicloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.

US Patent:

6649598, Nov 18, 2003

Filed:

Sep 7, 2001

Appl. No.:

09/949444

Inventors:

Steven M. Menchen - Fremont CA
Linda G. Lee - Palo Alto CA
Charles R. Connell - Redwood City CA
N. Davis Hershey - San Carlos CA
Vergine Chakerian - San Mateo CA
Sam L. Woo - Redwood City CA
Steven Fung - Palo Alto CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

A01N 4304

US Classification:

514 44, 435 6, 435 91, 435968, 536 231, 536 243, 536 2532, 536 254, 536 256, 436 86, 436 94, 436 96, 436 98, 436161, 436162, 436800, 935 77

Abstract:

Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting specially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2â,4â,5â,7â-tetrachloro-4,7-dichloro-5-(and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.

US Patent:

6743905, Jun 1, 2004

Filed:

Apr 16, 2001

Appl. No.:

09/836704

Inventors:

Sam L. Woo - Redwood City CA
Ron Graham - San Ramon CA
Jing Tian - Mountain View CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

C07H 2100

US Classification:

536 231, 435 6, 536 253, 536 266

Abstract:

The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.

US Patent:

6756204, Jun 29, 2004

Filed:

Jun 10, 2002

Appl. No.:

10/170044

Inventors:

Paul David Grossman - Hillsborough CA
Steven Fung - Palo Alto CA
Steven Michael Menchen - Fremont CA
Sam Lee Woo - Redwood City CA
Emily Susan Winn-Deen - Pleasanton CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912

Abstract:

Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.

US Patent:

6759202, Jul 6, 2004

Filed:

Jun 10, 2002

Appl. No.:

10/167337

Inventors:

Paul David Grossman - Hillsborough CA
Steven Fung - Palo Alto CA
Steven Michael Menchen - Fremont CA
Sam Lee Woo - Redwood City CA
Emily Susan Winn-Deen - Foster City CA

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912

Abstract:

Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.

US Patent:

7074569, Jul 11, 2006

Filed:

May 28, 2004

Appl. No.:

10/856752

Inventors:

Sam Lee Woo - Redwood City CA, US
Ronald J. Graham - San Ramon CA, US
Jing Tian - Mountain View CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 1/68
C07H 21/00
C07H 21/02

US Classification:

435 6, 536 231, 536 253, 536 266

Abstract:

The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.

US Patent:

7115376, Oct 3, 2006

Filed:

Apr 14, 2004

Appl. No.:

10/825074

Inventors:

Paul David Grossman - Burlingame CA, US
Steven Fung - Palo Alto CA, US
Steven Michael Menchen - Fremont CA, US
Sam Lee Woo - Redwood City CA, US
Emily Susan Winn-Deen - Foster City CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 911, 435 912

Abstract:

Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.

US Patent:

7129050, Oct 31, 2006

Filed:

Apr 14, 2004

Appl. No.:

10/825624

Inventors:

Paul David Grossman - Burlingame CA, US
Steven Fung - Palo Alto CA, US
Steven Michael Menchen - Fremont CA, US
Sam Lee Woo - Redwood City CA, US
Emily Susan Winn-Deen - Foster City CA, US

Assignee:

Applera Coporation - Foster City CA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 911, 435 912

Abstract:

Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.