East Carolina University Aug 2012 - May 2017
Graduate Student-Phd
Abc Laboratories Sep 2011 - Aug 2012
Associate Scientist
Southern Illinois University Aug 2008 - May 2011
Graduate Student
Johnson & Johnson Vision Care, Inc. Sep 2006 - Jul 2008
Microbiologist
Education:
East Carolina University 2017 - 2019
Masters
The Brody School of Medicine at East Carolina University 2012 - 2017
Doctorates, Doctor of Philosophy, Biology, Anatomy, Philosophy
Southern Illinois University, Carbondale 2008 - 2011
Master of Science, Masters, Molecular Biology, Biochemistry, Microbiology
University of Florida 2003 - 2006
Bachelors, Bachelor of Science, Microbiology
Skills:
Glp Elisa Pcr Mammalian Cell Culture Sds Page Aseptic Technique Gmp Microscopy Western Blotting Dna Extraction Sop Development Method Development Qpcr Bacterial Culture Microsoft Office Cell Culture Uv/Vis Biotechnology Validation Rna Isolation Bioassay Clustalw Softmax Pro Facs Analysis Transduction Transfection
Southern Illinois University Carbondale, IL Aug 2008 to May 2011 o Graduate AssistantVistakon, INC through Kelly Scientific Services Jacksonville, FL Sep 2006 to Jul 2008 o MicrobiologistUniversity of Florida Gainesville, FL Jan 2005 to May 2006 o Research Assistant
Education:
Southern Illinois University May 2011 Master of Science in Molecular Biology, Microbiology, BiochemistryUniversity of Florida Gainesville, FL Jan 2003 to Jan 2006 Bachelor of Science in Microbiology and Cell Sciences
Skills:
Aseptic technique, Nucleic acid extraction (plasmid, DNA, RNA), RT-PCR, PCR & primer design, Plasmid cloning, Electroporation, Triparental mating, Mammalian cell maintenance, Lysate and membrane bound protein extraction, BCA protein assay, Western Immunoblots, SDS-PAGE, Bacterial culturing, isolation, and identification Method/material qualification Light microscopy Bacterial smear preparation and staining Serial dilutions Growth media preparation Bacterial enumeration through optical density and plating Writing technical reports Presenting findings to supervisors and peers GMP/GLP and ISO standards Software MS Word MS Excel MS PowerPoint MS Outlook Adobe Photoshop Scion ImageSoftware ACT-1 analySIS FIVE ProtoCOL Equipment Centrifuge Gradient thermocycler Spectrophotometer BTX Electrocell electroporator Electrophoresis rigs Autoclave Light Microscope (oil immersion) Cytoviva
Bosque Farms Elementary School Bosque Farms NM 1980-1984, Puesta Del Sol Elementary School Albuquerque NM 1984-1986, Lincoln Middle School Rio Rancho NM 1986-1989
Community:
Daniel Hine
Biography:
Life
Happily married with a little boy, was in the US Navy from 2000 - 2003, and now...