Sandra L Jacobson

US Patent:

20230104032, Apr 6, 2023

Filed:

Sep 30, 2022

Appl. No.:

17/957633

Inventors:

- Visalia CA, US
Sandra Jacobson - El Cajon CA, US
Kris Ramos - Sanger CA, US
Prachand Shrestha - Visalia CA, US

Assignee:

Edeniq, Inc. - Visalia CA

International Classification:

C12P 19/14
C13K 13/00
C07H 3/04
C12P 19/12
C12P 19/02
C13K 1/02
C12P 7/10

Abstract:

The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatment. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.

US Patent:

20190276865, Sep 12, 2019

Filed:

Mar 21, 2019

Appl. No.:

16/360408

Inventors:

- Visalia CA, US
Sandra Jacobson - El Cajon CA, US
Kris Ramos - Sanger CA, US
Prachand Shrestha - Visalia CA, US

International Classification:

C12P 19/14
C13K 1/02
C07H 3/04
C12P 19/12
C12P 7/10
C13K 13/00
C12P 19/02

Abstract:

The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatement. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose.

US Patent:

20160160252, Jun 9, 2016

Filed:

Jul 24, 2014

Appl. No.:

14/907212

Inventors:

- Visalia CA, US
Sandra Jacobson - El Cajon CA, US
Kristoffer Ramos - Sanger CA, US
Mrugesh Patel - Norton VA, US
James Kacmar - Visalia CA, US
Daniel Michalopoulos - Exeter CA, US

International Classification:

C12P 19/14
C12P 19/02
C08F 126/10
C08F 116/06
C08G 65/08
C08G 65/34

Abstract:

Methods and systems for increasing the yield of sugars from a biomass, such as a lignocellulosic biomass, are described. A non-ionic organic polymer is contacted with the biomass during the saccharification reaction, and the hydrolyzed mixture is separated using a filter into a permeate and a retentate, where the non-ionic organic polymer is present in the retentate. The retentate with the polymer is recycled to the hydrolysis mixture, which increased the yield of sugars using less saccharification enzymes. The methods thus allow for increased cost savings by reducing the amount of enzymes required to convert the biomass to sugars.