Skills:
Molecular techniques Protein purification and characterization (antibodies and other proteins), IC50, AUC studies for drugs, Immunoassays (Western, ELISA, MSD, Immunofluorescence), Flow cytometry (FACs), Real-Time PCR, Immunoprecipitation assay (IP and ChIP), Proteomics, Regular and viral (adeno, retro, lenti, baculoviral) transfections (siRNA, shRNA and vectors), microscopy (confocal and timelapse), Basic Molecular Biology techniques (Engineering expression constructs/cells, site-directed mutagenesis, luciferase assay), Cell culture and Cell Cycle techniques, Apoptotic techniques, clonogenic and soft agar colony assays, 3-D morphogenesis assays, anoikis, Cancer stem cell techniques (staining for biomarkers, tumorspheres assay etc). In vivo work experience Xenograft studies for therapeutic efficacy, bone marrow studies for reconstitution and viral titer determination, BrdU incorporation, animal surgeries, therapeutic biomarker studies, cancer stem cell biomarker analysis. Writing/Reviewing skills Multiple NIH-RO1 grant writings in collaboration with laboratory PI Served as reviewer for several journals Software SAS, Adobe photoshop, Endnote, ImageJ/Fiji, FloJo (FACs analysis software), fluorescence microscopy (Zeiss and Nikon), Microsoft Office.