Sara S Gutierrez

US Patent:

7166225, Jan 23, 2007

Filed:

Mar 22, 2005

Appl. No.:

11/086249

Inventors:

Aldo M. Pitt - Wayland MA, US
Sara D. Gutierrez - Danvers MA, US
Thomas G. Zermani - Peabody MA, US
Jeffrey Busnach - Billerica MA, US
Joseph J. Raneri - Tewksbury MA, US
James C. Groves - Gloucester MA, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B01D 61/14
G01N 1/18

US Classification:

210650, 210781, 436178

Abstract:

A fluid filtering device includes a plurality of filtering wells and an angling mechanism which adjusts the angle of the membranes within the filtering wells relative to a line of a centrifuge where the line is perpendicular to the axis of rotation of a centrifuge and passes through the center of a major plane of the filtering device. The angling mechanism can include a wedge located between the center of rotation of a centrifuge and a swinging bucket of the centrifuge. The angling mechanism can also be located within each filtering well and can provide individually specified angles for each membrane, relative to the line of the centrifuge.

US Patent:

20030199001, Oct 23, 2003

Filed:

Apr 21, 2003

Appl. No.:

10/419374

Inventors:

Aldo Pitt - Wayland MA, US
Sara Gutierrez - Danvers MA, US

International Classification:

G01N033/53
C12N009/00
C07K014/00
C07K001/34

US Classification:

435/007100, 435/183000, 530/414000

Abstract:

A system, kit and process for the separation, recovery, purification and identification of biomarkers such as peptides and the like from serum or plasma. The process is to filter the sample through a UF filtration device optionally having a vertical or substantial vertical ultrafilter membrane, especially when in a single well configuration device. The filtrate is recovered and if desired desalted using various chromatography media such as reverse phase and ion exchange media. The sample is then applied to a MALDI/TOF target or HPLC column and analyzed in a mass spectrometer the presence, absence or variation of the biomarkers. Higher recovery and concentration of these biomarkers is achieved with the present invention.

US Patent:

20050133425, Jun 23, 2005

Filed:

Feb 3, 2005

Appl. No.:

11/050251

Inventors:

Aldo Pitt - Wayland MA, US
Sara Gutierrez - Danvers MA, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B01D015/08

US Classification:

210198200, 210321600, 422061000, 422070000, 422101000

Abstract:

A system, kit and process for the separation, recovery, purification and identification of biomarkers such as peptides and the like from serum or plasma. The process is to filter the sample through a UF filtration device optionally having a vertical or substantial vertical ultrafilter membrane, especially when in a single well configuration device. The filtrate is recovered and if desired desalted using various chromatography media such as reverse phase and ion exchange media. The sample is then applied to a MALDI/TOF target or HPLC column and analyzed in a mass spectrometer the presence, absence or variation of the biomarkers. Higher recovery and concentration of these biomarkers is achieved with the present invention.

US Patent:

20130017620, Jan 17, 2013

Filed:

Jun 27, 2012

Appl. No.:

13/534570

Inventors:

Christopher A. Scott - Westford MA, US
Timothy K. Nadler - Lexington MA, US
Kurt Greenizen - Atkinson NH, US
Louis Bonhomme - Waltham MA, US
Sara D. Gutierrez - Danvers MA, US
Masaharu Mabuchi - Beverly MA, US
David Briggs - Douglas MA, US
Phillip Clark - Wakefield MA, US
Ralph T. Scaduto - Georgetown MA, US

Assignee:

EMD MILLIPORE CORPORATION - Billerica MA

International Classification:

G01N 1/34

US Classification:

436177, 422534

Abstract:

Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.

US Patent:

20140017151, Jan 16, 2014

Filed:

Sep 19, 2013

Appl. No.:

14/031458

Inventors:

Timothy K. Nadler - Lexington MA, US
Kurt Greenizen - Atkinson NH, US
Louis Bonhomme - Waltham MA, US
Sara D. Gutierrez - Danvers MA, US
Masaharu Mabuchi - Beverly MA, US
David Briggs - Douglas MA, US
Phillip Clark - Wakefield MA, US
Ralph T. Scaduto - Georgetown MA, US

Assignee:

EMD Millipore Corporation - Billerica MA

International Classification:

G01N 1/34

US Classification:

422534

Abstract:

Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.

US Patent:

6899810, May 31, 2005

Filed:

Aug 11, 2000

Appl. No.:

09/637403

Inventors:

Aldo M. Pitt - Wayland MA, US
Sara D. Gutierrez - Danvers MA, US
Thomas G. Zermani - Peabody MA, US
Jeffrey Busnach - Billerica MA, US
Joseph J. Raneri - Tewksbury MA, US
James C. Groves - Gloucester MA, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B01D063/08
B01D063/16

US Classification:

21032168, 210324, 2103801, 422101

Abstract:

A fluid filtering device includes a plurality of filtering wells and an angling mechanism which adjusts the angle of the membranes within the filtering wells relative to a line of a centrifuge where the line is perpendicular to the axis of rotation of a centrifuge and passes through the center of a major plane of the filtering device. The angling mechanism can include a wedge located between the center of rotation of a centrifuge and a swinging bucket of the centrifuge. The angling mechanism can also be located within each filtering well and can provide individually specified angles for each membrane, relative to the line of the centrifuge.

US Patent:

20160103045, Apr 14, 2016

Filed:

Dec 18, 2015

Appl. No.:

14/974334

Inventors:

- Billerica MA, US
Timothy K. Nadler - Lexington MA, US
Kurt Greenizen - Atkinson NH, US
Louis Bonhomme - Waltham MA, US
Sara D. Gutierrez - Danvers MA, US
Masaharu Mabuchi - Beverly MA, US
David Briggs - Douglas MA, US
Phillip Clark - Wakefield MA, US
Ralph T. Scaduto - Georgetown MA, US

International Classification:

G01N 1/34
G01N 1/40

Abstract:

Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.

US Patent:

20150260621, Sep 17, 2015

Filed:

Feb 10, 2015

Appl. No.:

14/618007

Inventors:

- Billerica MA, US
Paul Sydlowski - Danvers MA, US
John Doyle - Kensington NH, US
Ryan Amara - Tewksbury MA, US
Libby Kellard - Danvers MA, US
Sara Gutierrez - Danvers MA, US
Marc Emerick - Arlington MA, US

International Classification:

G01N 1/31

Abstract:

Apparatus and method for Immunohistochemistry, including a slide holder assembly fixture, a slide holder, a slide holder frame, and a slide holder frame assembly adapted to be placed in communication with a driving force to draw fluid through the slide holder. The slide holder may include an injection port that allows consistent fluid delivery and recovery, while minimizing the introduction of air bubbles. The slide holder may include a reservoir that allows for filling, soaking, rinsing, flushing, uniform antibody coverage of the tissue and reduces handling time, space, and errors, and one or more sample chambers. The slide orientation in the slide holder allows for visualization of label information and tissue to confirm adequate antibody coverage. A multi-port frame allows multiple assays to be carried out concurrently, and is removable from a vacuum manifold and allows for easy transport from the vacuum manifold to an incubator/refrigerator and back.