Sarah E Knight

US Patent:

20110070166, Mar 24, 2011

Filed:

Sep 28, 2010

Appl. No.:

12/892379

Inventors:

Niren Murthy - Atlanta GA, US
W. Robert Taylor - Stone Mountain GA, US
Kousik Kundu - Atlanta GA, US
Sarah F. Knight - Atlanta GA, US
Sungmun Lee - Dunwoody GA, US

International Classification:

A61K 49/00
C07D 403/06
C12Q 1/02
G01N 33/50
B65D 69/00

US Classification:

424 96, 548427, 435 29, 436127, 548455, 206223

Abstract:

Reduced dyes, such as hydrocyanines, deuterocyanines, and/or other deuterated dyes capable of detecting one or more reactive oxygen species are described herein. The reduced dyes exhibit little or no fluorescence due to the disrupted π conjugation. However, upon reaction with ROS, the reduced dyes are oxidized, regenerating the extended π conjugation and causing a substantial increase in fluorescence intensity. In many case, the oxidized dye is generally membrane impermeable. However, upon reduction, many of the reduced dyes are membrane permeable. Thus, reduced dyes can accumulate in cells and/or tissue to amplify the signal. Once inside the cell or tissue, the reduced dye is reoxidized upon reaction with ROS, and the oxidized dye again becomes membrane impermeable, trapping the dye within the cell. The reduced dyes can be used to image ROS, such as hydroxide radical and superoxide, in serum, cell cultures, tissue explants, and in vivo.

US Patent:

20120302575, Nov 29, 2012

Filed:

Sep 21, 2010

Appl. No.:

13/497398

Inventors:

W. Robert Taylor - Stone Mountain GA, US
Sarah F. Knight - Atlanta GA, US
Niren Murthy - Atlanta GA, US
Kousik Kundu - Atlanta GA, US

Assignee:

GEORGIA TECH RESEARCH CORPORATION - Atlanta GA
EMORY UNIVERSITY - Atlanta GA

International Classification:

A61K 31/519
A61P 13/12
C12N 5/071
A61P 9/10
C07D 475/04
A01N 1/02

US Classification:

514249, 544258, 435 11, 435374

Abstract:

The present disclosure is generally related to methods of preventing oxidative stress injury induced by renal is-chemia-reperfusion by a Folate-TEMPOL conjugate.

US Patent:

20140105826, Apr 17, 2014

Filed:

Dec 20, 2013

Appl. No.:

14/136068

Inventors:

- Atlanta GA, US
Kousik Kundu - Lincoln NE, US
Sarah F. Knight - Atlanta GA, US
Sungmun Lee - Dunwoody GA, US

Assignee:

Emory University - Atlanta GA
Georgia Tech Research Corporation - Atlanta GA

International Classification:

A61K 49/00
G01N 33/50

US Classification:

424 96, 435 29

Abstract:

Reduced dyes, such as hydrocyanines, deuterocyanines, and/or other deuterated dyes capable of detecting one or more reactive oxygen species are described herein. The reduced dyes exhibit little or no fluorescence due to the disrupted π conjugation. However, upon reaction with ROS, the reduced dyes are oxidized, regenerating the extended π conjugation and causing a substantial increase in fluorescence intensity. In many case, the oxidized dye is generally membrane impermeable. However, upon reduction, many of the reduced dyes are membrane permeable. Thus, reduced dyes can accumulate in cells and/or tissue to amplify the signal. Once inside the cell or tissue, the reduced dye is reoxidized upon reaction with ROS, and the oxidized dye again becomes membrane impermeable, trapping the dye within the cell. The reduced dyes can be used to image ROS, such as hydroxide radical and superoxide, in serum, cell cultures, tissue explants, and in vivo.