Scott G Saunders

US Patent:

6699968, Mar 2, 2004

Filed:

Nov 28, 2000

Appl. No.:

09/723677

Inventors:

Scott Saunders - Creve Coeur MO
Merton Bernfield - Boston MA
Masato Kato - Hawthorne, AU

Assignee:

Childrens Medical Center Corporation - Boston MA

International Classification:

C07K 100

US Classification:

530350, 530395, 530399, 530402, 435 691

Abstract:

A purified mammalian proteoglycan, and genetic information encoding such proteoglycans, having a core polypetide molecular weight of about 30 kD to about 35 kD, and comprising a hydrophilic amino terminal extracellular region, a hydrophilic carboxy terminal cytoplasmic region, a transmembrane hydrophobic region between said cytoplasmic and extracellular regions, a protease susceptible cleavage sequence extracellularly adjacent the transmembrane region of the peptide, and at least one glycosylation site for attachment of a heparan sulfate chain to said extracellular region, said glycosylation site comprising a heparan sulfate attachment sequence represented by a formula Xac-Z-Ser-Gly-Ser-Gly (SEQ ID NO: 44), where Xac represents an amino acid residue having an acidic sidechain, and Z represents from 1 to 10 amino acid residues. Additional peptides having this glycosylation site and genetic information useful for preparing a number of variations based on this glycosylation site are also provided.

US Patent:

7183393, Feb 27, 2007

Filed:

Feb 11, 2004

Appl. No.:

10/776989

Inventors:

Scott Saunders - Creve Coeur MO, US
Merton Bernfield - Boston MA, US
Masato Kato - Hawthorne, AU

Assignee:

Children's Medical Center Corporation - Boston MA

International Classification:

C07K 14/00
C07K 19/00
A61K 38/00
A61K 38/03
A61K 38/16
C12P 21/00

US Classification:

530395, 530350, 530399, 530402, 435 691, 435 697

Abstract:

A purified mammalian proteoglycan, and genetic information encoding such proteoglycans, having a core polypetide molecular weight of about 30 kD to about 35 kD, and comprising a hydrophilic amino terminal extracellular region, a hydrophilic carboxy terminal cytoplasmic region, a transmembrane hydrophobic region between said cytoplasmic and extracellular regions, a protease susceptible cleavage sequence extracellularly adjacent the transmembrane region of the peptide, and at least one glycosylation site for attachment of a heparan sulfate chain to said extracellular region, said glycosylation site comprising a heparan sulfate attachment sequence represented by a formula Xac-Z-Ser-Gly-Ser-Gly (SEQ ID NO: 44), where Xac represents an amino acid residue having an acidic sidechain, and Z represents from 1 to 10 amino acid residues. Additional peptides having this glycosylation site and genetic information useful for preparing a number of variations based on this glycosylation site are also provided.