Skills:
Computer Skills: Proficient with Windows, Mac, and Linux/Unix-based operating systems, MS Office suite, database management programs, statistical and graphing packages, bioinformatics, microarray and gene network analysis resources, and quantitative image analysis software. Molecular Biology: Cloning and sub-cloning, PCR, purification of DNA and RNA, sequencing, multiplex RNase protection assay and probe design, cDNA and Affymetrix arrays, QRT-PCR, allelic discrimination (microsatellite; Taqman SNP assays). Cell Biology and Biochemistry: Mammalian cell culture, transfection, lentiviral transduction, stable cell line generation, reporter assay design, histone acid extraction, immunoprecipitations, immunohistochemistry, immunofluorescence, and in situ hybridization. Antibody generation in rabbits, protein column chromatography, protease assays, zymography, radioimmunoassay, SDS-PAGE, silver stains and western blots. Mouse Biology: Handling and husbandry, genotyping, and phenotypic characterization of transgenic mouse models. Intraperitoneal and tail vein injections, oral gavage, intracardiac perfusion, mammary fat pad orthotopic implantation, general mouse dissection, prostate microdissection, ex vivo lung culture metastasis assays, use of Xenogen IVIS and GE uCT systems for in vivo imaging. Microscopy: Bright field, fluorescence, laser scanning confocal, deconvolution and reconstruction.