Sean Christopher Mccarthy

US Patent:

6391586, May 21, 2002

Filed:

Nov 7, 2000

Appl. No.:

09/707802

Inventors:

Sean Anthony McCarthy - Boston MA
David Paul Gearing - Wellesley MA
Douglas Adam Levinson - Sherborn MA

Assignee:

Millennium Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12P 2102

US Classification:

435 691, 435243, 4353201, 435325, 435420, 536 231, 536 235

Abstract:

The invention features a method for identifying a cDNA nucleic acid encoding a mammalian protein having a signal sequence, which method includes the following steps: (a) providing library of mammalian cDNA; (b) ligating the library of mammalian cDNA to DNA encoding alkaline phosphatase lacking both a signal sequence and a membrane anchor sequence to form ligated DNA; (c) transforming bacterial cells with the ligated DNA to create a bacterial cell clone library; (d) isolating DNA comprising the mammalian cDNA from at least one clone in the bacterial cell clone library; (e) separately transfecting DNA isolated from clones in step (d) into mammalian cells which do not express alkaline phosphatase to create a mammalian cell clone library wherein each clone in the mammalian cell clone library corresponds to a clone in the bacterial cell clone library; (f) identifying a clone in the mammalian cell clone library which expresses alkaline phosphatase; (g) identifying the clone in the bacterial cell clone library corresponding to the clone in the mammalian cell clone library identified in step (f); and (h) isolating and sequencing a portion of the mammalian cDNA present in the bacterial cell library clone identified in step (g) to identify a mammalian cDNA encoding a mammalian protein having a signal sequence.

US Patent:

6395872, May 28, 2002

Filed:

Nov 21, 2001

Appl. No.:

09/991326

Inventors:

Sean Anthony McCarthy - Boston MA
David Paul Gearing - Welleslev MA
Douglas Adam Levinson - Sherborn MA

Assignee:

Millennium Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C07K 700

US Classification:

530300, 530324, 530325, 530326, 530350

Abstract:

The invention features a method for identifying a cDNA nucleic acid encoding a mammalian protein having a signal sequence, which method includes the following steps: (a) providing library of mammalian cDNA; (b) ligating the library of mammalian cDNA to DNA encoding alkaline phosphatase lacking both a signal sequence and a membrane anchor sequence to form ligated DNA; 8 transforming bacterial cells with the ligated DNA to create a bacterial cell clone library; (d) isolating DNA comprising the mammalian cDNA from at least one clone in the bacterial cell clone library; (e) separately transfecting DNA isolated from clones in step (d) into mammalian cells which do not express alkaline phosphatase to create a mammalian cell clone library wherein each clone in the mammalian cell clone library corresponds to a clone in the bacterial cell clone library; (f) identifying a clone in the mammalian cell clone library which expresses alkaline phosphatase; (g) identifying the clone in the bacterial cell clone library corresponding to the clone in the mammalian cell clone library identified in step (f); and (h) isolating and sequencing a portion of the mammalian cDNA present in the bacterial cell library clone identified in step (g) to identify a mammalian cDNA encoding a mammalian protein having a signal sequence.

US Patent:

6410315, Jun 25, 2002

Filed:

Nov 8, 1999

Appl. No.:

09/436183

Inventors:

Sean A. McCarthy - Boston MA
Steven Bossone - Lexington MA

Assignee:

Millennium Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12N 1563

US Classification:

4353201, 435425, 536 231

Abstract:

The invention involves a method of identifying nucleic acid sequences encoding signal peptide-containing proteins. The method features chimeric constructs containing a KRE9 gene that lacks a signal sequence. Yeast containing chimeric KRE9 plasmid constructs that encode signal sequences are selected based on their ability to grow on media in which sucrose is the sole carbon source.

US Patent:

7033780, Apr 25, 2006

Filed:

Jun 14, 1999

Appl. No.:

09/333159

Inventors:

Sean A. McCarthy - Boston MA, US
Christopher C. Fraser - Lexington MA, US
John D. Sharp - Arlington MA, US
Thomas M. Barnes - Boston MA, US

Assignee:

Millennium Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12N 15/00

US Classification:

435 691, 4353201, 4352523, 435325, 435 6, 536 232, 536 235, 530350

Abstract:

The invention provides isolated nucleic acids encoding a variety of proteins having diagnostic, preventive, therapeutic, and other uses. These nucleic and proteins are useful for diagnosis, prevention, and therapy of a number of human and other animal disorders. The invention also provides antisense nucleic acid molecules, expression vectors containing the nucleic acid molecules of the invention, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a nucleic acid molecule of the invention has been introduced or disrupted. The invention still further provides isolated polypeptides, fusion polypeptides, antigenic peptides and antibodies. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided. The nucleic acids and polypeptides of the present invention are useful as modulating agents in regulating a variety of cellular processes.

US Patent:

D649582, Nov 29, 2011

Filed:

Feb 11, 2011

Appl. No.:

29/385337

Inventors:

Sean McCarthy - East Northport NY, US

International Classification:

1703

US Classification:

D17 14

US Patent:

D649585, Nov 29, 2011

Filed:

Feb 11, 2011

Appl. No.:

29/385332

Inventors:

Sean McCarthy - East Northport NY, US

International Classification:

1703

US Classification:

D17 20

US Patent:

8571899, Oct 29, 2013

Filed:

Jul 20, 2012

Appl. No.:

13/554935

Inventors:

Robert Phillips Cochran - New York NY, US
Sean Wallace McCarthy - New York NY, US

Assignee:

HG Global Ltd. - Hamilton HM

International Classification:

G06Q 30/00

US Classification:

705 4

Abstract:

A computer includes a processor and a memory storing data and executable instructions to operate a municipal financial guaranty mutual insurance company. The processor processes the data and executable instructions to track insurance premiums paid by members of the municipal financial guaranty mutual insurance company, monitor private investment capital into a holding firm, follow the investment of a first subset of the private investment capital of the holding firm into the municipal financial guaranty mutual insurance company in exchange for first promissory notes, track a second subset of the private investment capital of the holding firm into a reinsurance firm, follow the investment of a segment of the second subset of the private investment capital of the reinsurance firm into the municipal financial guaranty mutual insurance company in exchange for second promissory notes, and manage reinsurance firm first loss claims from the municipal financial guaranty mutual insurance company.

US Patent:

20010041353, Nov 15, 2001

Filed:

Jan 5, 2001

Appl. No.:

09/755958

Inventors:

Sean McCarthy - Boston MA, US

Assignee:

Millennium Biotherapeutics

International Classification:

C12Q001/68
A61K038/43
C12N009/16
C12P021/02
C12N005/06
C07H021/04

US Classification:

435/069100, 435/196000, 435/325000, 435/006000, 424/094100, 536/023200

Abstract:

The present invention relates to the discovery of novel genes encoding Small Secreted Protein-1 (SSP-1) polypeptides. Therapeutics, diagnostics and screening assays based on these molecules are also disclosed.