Sean P Mccarthy

US Patent:

6391586, May 21, 2002

Filed:

Nov 7, 2000

Appl. No.:

09/707802

Inventors:

Sean Anthony McCarthy - Boston MA
David Paul Gearing - Wellesley MA
Douglas Adam Levinson - Sherborn MA

Assignee:

Millennium Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12P 2102

US Classification:

435 691, 435243, 4353201, 435325, 435420, 536 231, 536 235

Abstract:

The invention features a method for identifying a cDNA nucleic acid encoding a mammalian protein having a signal sequence, which method includes the following steps: (a) providing library of mammalian cDNA; (b) ligating the library of mammalian cDNA to DNA encoding alkaline phosphatase lacking both a signal sequence and a membrane anchor sequence to form ligated DNA; (c) transforming bacterial cells with the ligated DNA to create a bacterial cell clone library; (d) isolating DNA comprising the mammalian cDNA from at least one clone in the bacterial cell clone library; (e) separately transfecting DNA isolated from clones in step (d) into mammalian cells which do not express alkaline phosphatase to create a mammalian cell clone library wherein each clone in the mammalian cell clone library corresponds to a clone in the bacterial cell clone library; (f) identifying a clone in the mammalian cell clone library which expresses alkaline phosphatase; (g) identifying the clone in the bacterial cell clone library corresponding to the clone in the mammalian cell clone library identified in step (f); and (h) isolating and sequencing a portion of the mammalian cDNA present in the bacterial cell library clone identified in step (g) to identify a mammalian cDNA encoding a mammalian protein having a signal sequence.

US Patent:

6395872, May 28, 2002

Filed:

Nov 21, 2001

Appl. No.:

09/991326

Inventors:

Sean Anthony McCarthy - Boston MA
David Paul Gearing - Welleslev MA
Douglas Adam Levinson - Sherborn MA

Assignee:

Millennium Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C07K 700

US Classification:

530300, 530324, 530325, 530326, 530350

Abstract:

The invention features a method for identifying a cDNA nucleic acid encoding a mammalian protein having a signal sequence, which method includes the following steps: (a) providing library of mammalian cDNA; (b) ligating the library of mammalian cDNA to DNA encoding alkaline phosphatase lacking both a signal sequence and a membrane anchor sequence to form ligated DNA; 8 transforming bacterial cells with the ligated DNA to create a bacterial cell clone library; (d) isolating DNA comprising the mammalian cDNA from at least one clone in the bacterial cell clone library; (e) separately transfecting DNA isolated from clones in step (d) into mammalian cells which do not express alkaline phosphatase to create a mammalian cell clone library wherein each clone in the mammalian cell clone library corresponds to a clone in the bacterial cell clone library; (f) identifying a clone in the mammalian cell clone library which expresses alkaline phosphatase; (g) identifying the clone in the bacterial cell clone library corresponding to the clone in the mammalian cell clone library identified in step (f); and (h) isolating and sequencing a portion of the mammalian cDNA present in the bacterial cell library clone identified in step (g) to identify a mammalian cDNA encoding a mammalian protein having a signal sequence.

US Patent:

6410315, Jun 25, 2002

Filed:

Nov 8, 1999

Appl. No.:

09/436183

Inventors:

Sean A. McCarthy - Boston MA
Steven Bossone - Lexington MA

Assignee:

Millennium Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12N 1563

US Classification:

4353201, 435425, 536 231

Abstract:

The invention involves a method of identifying nucleic acid sequences encoding signal peptide-containing proteins. The method features chimeric constructs containing a KRE9 gene that lacks a signal sequence. Yeast containing chimeric KRE9 plasmid constructs that encode signal sequences are selected based on their ability to grow on media in which sucrose is the sole carbon source.

US Patent:

7033780, Apr 25, 2006

Filed:

Jun 14, 1999

Appl. No.:

09/333159

Inventors:

Sean A. McCarthy - Boston MA, US
Christopher C. Fraser - Lexington MA, US
John D. Sharp - Arlington MA, US
Thomas M. Barnes - Boston MA, US

Assignee:

Millennium Pharmaceuticals, Inc. - Cambridge MA

International Classification:

C12N 15/00

US Classification:

435 691, 4353201, 4352523, 435325, 435 6, 536 232, 536 235, 530350

Abstract:

The invention provides isolated nucleic acids encoding a variety of proteins having diagnostic, preventive, therapeutic, and other uses. These nucleic and proteins are useful for diagnosis, prevention, and therapy of a number of human and other animal disorders. The invention also provides antisense nucleic acid molecules, expression vectors containing the nucleic acid molecules of the invention, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a nucleic acid molecule of the invention has been introduced or disrupted. The invention still further provides isolated polypeptides, fusion polypeptides, antigenic peptides and antibodies. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided. The nucleic acids and polypeptides of the present invention are useful as modulating agents in regulating a variety of cellular processes.

US Patent:

20010041353, Nov 15, 2001

Filed:

Jan 5, 2001

Appl. No.:

09/755958

Inventors:

Sean McCarthy - Boston MA, US

Assignee:

Millennium Biotherapeutics

International Classification:

C12Q001/68
A61K038/43
C12N009/16
C12P021/02
C12N005/06
C07H021/04

US Classification:

435/069100, 435/196000, 435/325000, 435/006000, 424/094100, 536/023200

Abstract:

The present invention relates to the discovery of novel genes encoding Small Secreted Protein-1 (SSP-1) polypeptides. Therapeutics, diagnostics and screening assays based on these molecules are also disclosed.

US Patent:

20020028508, Mar 7, 2002

Filed:

Feb 21, 2001

Appl. No.:

09/790264

Inventors:

Douglas Holtzman - Jamaica Plain MA, US
Andrew Goodearl - Natick MA, US
Sean McCarthy - San Diego CA, US

International Classification:

G01N033/53
C07H021/02
C07H021/04
C12P021/06
C12N001/20
C12N005/00
C12N005/02
C07K001/00
C07K014/00
C07K017/00
C07K016/00

US Classification:

435/325000, 536/023100, 435/252300, 530/350000, 530/387100, 435/007100, 435/068100

Abstract:

The invention provides isolated nucleic acids encoding a variety of proteins having diagnostic, preventive, therapeutic, and other uses. These nucleic and proteins are useful for diagnosis, prevention, and therapy of a number of human and other animal disorders. The invention also provides antisense nucleic acid molecules, expression vectors containing the nucleic acid molecules of the invention, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a nucleic acid molecule of the invention has been introduced or disrupted. The invention still further provides isolated polypeptides, fusion polypeptides, antigenic peptides and antibodies. Diagnostic, screening, and therapeutic methods using compositions of the invention are also provided. The nucleic acids and polypeptides of the present invention are useful as modulating agents in regulating a variety of cellular processes.

US Patent:

20020072089, Jun 13, 2002

Filed:

Feb 13, 2001

Appl. No.:

09/782980

Inventors:

Douglas Holtzman - Jamaica Plain MA, US
Sean McCarthy - San Diego CA, US
Kyle MacBeth - Boston MA, US
Samantha Busfield - Maddington, AU
Yang Pan - Bellevue WA, US
David White - Braintree MA, US
Mehran Khodadoust - Brookline MA, US
Wei Gu - Brookline MA, US

International Classification:

C07H021/04
C12N009/00
C12N005/06
C12P021/02

US Classification:

435/069100, 435/183000, 435/325000, 435/320100, 536/023100

Abstract:

Novel ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST proteins, the invention further provides isolated ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST fusion proteins, antigenic peptides and anti- ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST antibodies. The invention also provides ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which an ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST gene has been introduced or disrupted. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided.

US Patent:

20020086354, Jul 4, 2002

Filed:

Mar 8, 2001

Appl. No.:

09/802582

Inventors:

Douglas Holtzman - Jamaica Plain MA, US
Andrew Goodearl - Natick MA, US
Sean McCarthy - San Diego CA, US

International Classification:

C07K014/705
C07H021/04
C12P021/02
C12N005/06

US Classification:

435/069100, 435/325000, 435/320100, 530/350000, 536/023500

Abstract:

The invention relates to Tango-73, Tango-74, Tango-76, Tango-78, and Tango-83 polypeptides, nucleic acid molecules encoding Tango-73, Tango-74, Tango-76, Tango-78, and Tango-83, and uses thereof. The invention provides isolated nucleic acids encoding a variety of proteins having diagnostic, preventive, therapeutic, and other uses. These nucleic and proteins are useful for diagnosis, prevention, and therapy of a number of human and other animal disorders. The invention also provides antisense nucleic acid molecules, expression vectors containing the nucleic acid molecules of the invention, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a nucleic acid molecule of the invention has been introduced or disrupted. The invention still further provides isolated polypeptides, fusion polypeptides, antigenic peptides and antibodies. Diagnostic, screening, and therapeutic methods using compositions of the invention are also provided. The nucleic acids and polypeptides of the present invention are useful as modulating agents in regulating a variety of cellular processes.