Sean P Morrison

US Patent:

20030003572, Jan 2, 2003

Filed:

Mar 5, 1999

Appl. No.:

09/263359

Inventors:

DAVID J. ANDERSON - ALTADENA CA, US
SEAN MORRISON - PASADENA CA, US

International Classification:

A61K048/00
C12N005/08

US Classification:

435/325000, 435/368000, 435/377000, 435/378000, 435/383000, 435/395000, 424/093210

Abstract:

The invention provides methods for the prospective identification, isolation, and self-renewal of neural stem cells from the mammalian peripheral nervous system and compositions of neural stem cells derived from uncultured tissue. Using flow-cytometry, neural crest derived cells of embryonic peripheral nerve were fractionated based on cell surface markers. The isolated p75Pcells from embryonic sciatic nerve were phenotypically and functionally indistinguishable from neural crest stem cells (NCSCs) previously isolated from neural tube explant cultures. Furthermore, freshly isolated p75Pcells gave rise to both neurons and glia when transplanted in vivo. Cell cycle analysis and BrdU labeling indicated that p75PNCSCs persist in the peripheral nerve by undergoing self-renewing divisions after neural crest migration has ceased.

US Patent:

20030099623, May 29, 2003

Filed:

Oct 24, 2002

Appl. No.:

10/279568

Inventors:

David Anderson - Altadena CA, US
Sean Morrison - Pasadena CA, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

A61K048/00
C12N005/08

US Classification:

424/093210, 435/368000

Abstract:

The invention provides methods for the prospective identification, isolation, and self-renewal of neural stem cells from the mammalian peripheral nervous system and compositions of neural stem cells derived from uncultured tissue. Using flow-cytometry, neural crest derived cells of embryonic peripheral nerve were fractionated based on cell surface markers. The isolated p75 Pcells from embryonic sciatic nerve were phenotypically and functionally indistinguishable from neural crest stem cells (NCSCs) previously isolated from neural tube explant cultures. Furthermore, freshly isolated p75 Pcells gave rise to both neurons and glia when transplanted in vivo. Cell cycle analysis and BrdU labeling indicated that p75 PNCSCs persist in the peripheral nerve by undergoing self-renewing divisions after neural crest migration has ceased.