Sheryl A Frank

US Patent:

6689578, Feb 10, 2004

Filed:

Dec 6, 2001

Appl. No.:

10/007022

Inventors:

Todd DeZwaan - Apex NC
Sze-Chung Lo - Durham NC
Sheryl Frank - Durham NC
Blaise Darveaux - Hillsborough NC
Sanjoy K. Mahanty - Chapel Hill NC
Ryan Heiniger - Raleigh NC
Amy Skalchunes - Raleigh NC
Huaqin Pan - Apex NC
Rex Tarpey - Apex NC
Jeffrey Shuster - Chapel Hill NC
Matthew M. Tanzer - Durham NC
Lisbeth Hamer - Durham NC
Kiichi Adachi - Durham NC

Assignee:

Paradigm Genetics, Inc. - Research Triangle Park NC

International Classification:

C12Q 118

US Classification:

435 32, 435 6, 435 911, 435 914, 435440, 4353201, 435183, 435 4051, 43525411, 4352542, 530 351, 424 94

Abstract:

The present inventors have discovered that 5-Aminolevulinate synthase is essential for fungal pathogenicity. Specifically, the inhibition of 5-Aminolevulinate synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, 5-Aminolevulinate synthase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit 5-Aminolevulinate synthase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

US Patent:

6723529, Apr 20, 2004

Filed:

Nov 9, 2001

Appl. No.:

10/011146

Inventors:

Matthew M. Tanzer - Durham NC
Jeffrey Shuster - Chapel Hill NC
Lisbeth Hamer - Durham NC
Kiichi Adachi - Durham NC
Todd M. DeZwaan - Apex NC
Sze-Chung Lo - Durham NC
Sheryl Frank - Durham NC
Blaise Darveaux - Hillsborough NC
Sanjoy K. Mahanty - Chapel Hill NC
Ryan Heiniger - Raleigh NC
Amy Skalchunes - Raleigh NC
Huaqin Pan - Apex NC
Rex Tarpey - Apex NC

Assignee:

Paradigm Genetics, Inc. - Research Triangle Park NC

International Classification:

C12Q 104

US Classification:

435 34, 435 25, 435 32, 530300

Abstract:

The present inventors have discovered that -Aminoadipate Reductase is essential for fungal pathogenicity. Specifically, the inhibition of -Aminoadipate Reductase gene expression in fungi results in no signs of successful infection or lesions. Thus, -Aminoadipate Reductase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit -Aminoadipate Reductase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

US Patent:

6733963, May 11, 2004

Filed:

Dec 6, 2001

Appl. No.:

10/010227

Inventors:

Lisbeth Hamer - Durham NC
Kiichi Adachi - Durham NC
Todd DeZwaan - Apex NC
Sze-Chung Lo - Durham NC
Sheryl Frank - Durham NC
Blaise Darveaux - Hillsborough NC
Sanjoy Mahanty - Chapel Hill NC
Ryan Heiniger - Raleigh NC
Amy Skalchunes - Raleigh NC
Huaqin Pan - Apex NC
Rex Tarpey - Apex NC
Jeffrey Shuster - Chapel Hill NC
Matthew M. Tanzer - Durham NC

Assignee:

Paradigm Genetics, Inc. - Research Triangle Park NC

International Classification:

C12Q 100

US Classification:

435 4, 435 691, 4352522, 514601, 514603, 514924, 536 231, 536 237

Abstract:

The present inventors have discovered that 3-Isopropylmalate dehydratase is essential for fungal pathogenicity. Specifically, the inhibition of 3-Isopropylmalate dehydratase gene expression in fungi results in no signs of successful infection or lesions. Thus, 3-Isopropylmalate dehydratase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit 3-Isopropylmalate dehydratase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

US Patent:

6740498, May 25, 2004

Filed:

Dec 6, 2001

Appl. No.:

10/010084

Inventors:

Kiichi Adachi - Durham NC
Todd DeZwaan - Apex NC
Sze-Chung Lo - Durham NC
Sheryl Frank - Durham NC
Blaise Darveaux - Hillsborough NC
Sanjoy Mahanty - Chapel Hill NC
Ryan Heiniger - Raleigh NC
Amy Skalchunes - Raleigh NC
Huaqin Pan - Apex NC
Rex Tarpey - Apex NC
Jeffrey Shuster - Chapel Hill NC
Matthew M. Tanzer - Durham NC
Lisbeth Hamer - Durham NC

Assignee:

Paradigm Genetics, Inc. - Research Triangle Park NC

International Classification:

C12Q 142

US Classification:

435 21, 435 32

Abstract:

The present inventors have discovered that histidinol-phosphatase is essential for fungal pathogenicity. Specifically, the inhibition of histidinol-phosphatase gene expression in fungi results in small, non-sporulating lesions and reduced pathogenicity. Thus, histidinol-phosphatase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit histidinol-phosphatase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

US Patent:

6806060, Oct 19, 2004

Filed:

Dec 7, 2001

Appl. No.:

10/011106

Inventors:

Sheryl Frank - Durham NC
Blaise Darveaux - Hillsborough NC
Sanjoy Mahanty - Chapel Hill NC
Ryan Heiniger - Raleigh NC
Amy Skalchunes - Raleigh NC
Huaqin Pan - Apex NC
Rex Tarpey - Apex NC
Jeffrey Shuster - Chapel Hill NC
Matthew M. Tanzer - Durham NC
Lisbeth Hamer - Durham NC
Kiichi Adachi - Durham NC
Todd DeZwaan - Apex NC
Sze-Chung Lo - Durham NC

Assignee:

Icoria, Inc. - Research Triangle Park NC

International Classification:

C12Q 118

US Classification:

435 32, 435 29, 435 24, 435 23, 435 4

Abstract:

The present inventors have discovered that Threonine synthase is essential for fungal pathogenicity. Specifically, the inhibition of Threonine synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, Threonine synthase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit Threonine synthase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

US Patent:

6852484, Feb 8, 2005

Filed:

Dec 10, 2001

Appl. No.:

10/012991

Inventors:

Sze-Chung Lo - Durham NC, US
Sheryl Frank - Durham NC, US
Blaise Darveaux - Hillsborough NC, US
Sanjoy Mahanty - Chapel Hill NC, US
Ryan Heiniger - Raleigh NC, US
Amy Skalchunes - Raleigh NC, US
Huaqin Pan - Apex NC, US
Rex Tarper - Apex NC, US
Jeffrey Shuster - Chapel Hill NC, US
Matthew M. Tanzer - Durham NC, US
Lisbeth Hamer - Durham NC, US
Kiichi Adachi - Durham NC, US
Todd M. DeZwaan - Apex NC, US

Assignee:

Icoria, Inc. - Research Triangle Park NC

International Classification:

C12Q001/00
C12P021/06
C12N001/20
C07H021/04
A31K031/18

US Classification:

435 4, 435 29, 435 32, 435 691, 4352522, 514 32, 514601, 514603, 514924, 536 231, 536 237

Abstract:

The present inventors have discovered that Asparagine Synthase is essential for fungal pathogenicity. Specifically, the inhibition of Asparagine Synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, Asparagine Synthase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit Asparagine Synthase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

US Patent:

20030180829, Sep 25, 2003

Filed:

Nov 9, 2001

Appl. No.:

10/011200

Inventors:

Jeffrey Shuster - Chapel Hill NC, US
Matthew Tanzer - Durham NC, US
Lisbeth Hamer - Durham NC, US
Kiichi Adachi - Durham NC, US
Todd DeZwaan - Apex NC, US
Sze-Chung Lo - Durham NC, US
Blaise Darveaux - Hillsborough NC, US
Sheryl Frank - Durham NC, US
Ryan Heiniger - Raleigh NC, US
Sanjoy Mahanty - Chapel Hill NC, US
Huaqin Pan - Apex NC, US
Amy Skalchunes - Raleigh NC, US
Rex Tarpey - Apex NC, US

International Classification:

C12Q001/18
C12Q001/00

US Classification:

435/032000, 435/004000

Abstract:

The present inventors have discovered that homocitrate synthase is essential for fungal pathogenicity. Specifically, the inhibition of homocitrate synthase gene expression in fungi results in no signs of successful infection or lesions. Thus, homocitrate synthase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit homocitrate synthase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.

US Patent:

20030224472, Dec 4, 2003

Filed:

May 13, 2003

Appl. No.:

10/437094

Inventors:

Ryan Heiniger - Raleigh NC, US
Amy Skalchunes - Raleigh NC, US
Huaqin Pan - Apex NC, US
Rex Tarpey - Apex NC, US
Jeffrey Shuster - Chapel Hill NC, US
Matthew Tanzer - Durham NC, US
Lisbeth Hamer - Durham NC, US
Kiichi Adachi - Tokyo, JP
Todd DeZwaan - Apex NC, US
Sze-Chung Lo - Shun Lee Estate, HK
Sheryl Frank - Durham NC, US
Blaise Darveaux - Hillsborough NC, US
Sanjoy Mahanty - Chapel Hill NC, US

International Classification:

C12Q001/48
C12Q001/18

US Classification:

435/015000, 435/032000

Abstract:

The present inventors have discovered that Putrescine Aminopropyltransferase (SPE3) is essential for normal fungal pathogenicity. Specifically, the inhibition of Putrescine Aminopropyltransferase gene expression in fungi results in reduced pathogenicity on their host organism, producing smaller lesions that fail to spread across a leaf surface. Thus, Putrescine Aminopropyltransferase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit Putrescine Aminopropyltransferase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.