Shuang Zhang

US Patent:

20030211523, Nov 13, 2003

Filed:

Jan 30, 2003

Appl. No.:

10/354931

Inventors:

Shuang Zhang - New York NY, US
Edward Ziff - New York NY, US
Jun Zhong - Somerset NJ, US

International Classification:

C12Q001/68
G01N033/53
G01N033/567
C12N001/18

US Classification:

435/006000, 435/007200, 435/254200

Abstract:

A method of identifying proteins able to interact via protein-protein interactions with a protein of interest, comprising (a) performing a first assay to identify cells expressing a reporter gene, wherein the first assay is transcription-based two-hybrid screening system; (b) obtaining DNA from the cells identified in step (a) via a PCR procedure; and (c) performing a second assay on the DNA obtained in step (b), wherein the second assay is a non-transcription-based two-hybrid screening system, to identify cells expressing a reporter gene, wherein positive cells identified in step (a) that are found to be positive in step (c) express a protein able to interact via protein-protein interactions with a protein of interest.