Siyuan Chen

US Patent:

20220325276, Oct 13, 2022

Filed:

May 16, 2016

Appl. No.:

15/156134

Inventors:

- San Francisco CA, US
Bill James PECK - Santa Clara CA, US
Andres FERNANDEZ - San Francisco CA, US
Siyuan CHEN - San Mateo CA, US
Pierre INDERMUHLE - Berkeley CA, US
Cheng-Hsien WU - Burlingame CA, US
Esteban TORO - Fremont CA, US

International Classification:

C12N 15/10

Abstract:

Methods and devices for cell-free sorting and cloning of nucleic acid libraries are provided herein.

US Patent:

20220325278, Oct 13, 2022

Filed:

Apr 13, 2022

Appl. No.:

17/720210

Inventors:

- South San Frncisco CA, US
Siyuan CHEN - San Mateo CA, US
Elian LEE - Union City CA, US
Nathan RAYNARD - San Francisco CA, US

International Classification:

C12N 15/10
C12N 9/00
C12N 9/12
C12P 19/34
C12Q 1/6869
C12Q 1/6855
C12Q 1/6811

Abstract:

Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.

US Patent:

20220277808, Sep 1, 2022

Filed:

Feb 17, 2022

Appl. No.:

17/674644

Inventors:

- South San Francisco CA, US
Siyuan CHEN - San Mateo CA, US
Ramsey Ibrahim ZEITOUN - San Francisco CA, US

International Classification:

G16B 25/20
C12N 15/10
G16B 35/20
G16B 40/00
C12Q 1/6827

Abstract:

Provided herein are compositions and methods for identifying genomic variants. Further provided herein are compositions and methods for capture of genomic sequences. Further provided herein are compositions and methods for capturing genomic DNA comprising single nucleotide polymorphisms.

US Patent:

20220243195, Aug 4, 2022

Filed:

Apr 15, 2022

Appl. No.:

17/721680

Inventors:

- South San Francisco CA, US
Siyuan CHEN - San Mateo CA, US
Ross KETTLEBOROUGH - San Francisco CA, US
Elian LEE - Union City CA, US
Nathan RAYNARD - San Mateo CA, US

International Classification:

C12N 15/10

Abstract:

Provided herein are methods, systems, and compositions for efficient nucleic acid assembly. Nucleic acid assembly may comprise assembly of variants comprising paired homology.

US Patent:

20230115861, Apr 13, 2023

Filed:

Aug 18, 2022

Appl. No.:

17/820856

Inventors:

- South San Francisco CA, US
Siyuan CHEN - San Mateo CA, US
Nathan RAYNARD - San Mateo CA, US

International Classification:

C12Q 1/6806
C12N 15/10
C12P 19/34

Abstract:

Provided herein are methods, systems, and compositions for assembly of covalently closed double stranded nucleic acids. Provided herein are methods, systems, and compositions for assembly covalently closed double stranded nucleic acids for use in various downstream processes.

US Patent:

20230086062, Mar 23, 2023

Filed:

Aug 9, 2022

Appl. No.:

17/818656

Inventors:

- South San Francisco CA, US
Bill James PECK - Santa Clara CA, US
Andres FERNANDEZ - San Francisco CA, US
Siyuan CHEN - San Mateo CA, US
Pierre INDERMUHLE - Berkeley CA, US

International Classification:

B01J 19/00
C12N 15/10
C40B 50/18
C12N 15/66
C12N 15/74

Abstract:

De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

US Patent:

20230067552, Mar 2, 2023

Filed:

Sep 19, 2022

Appl. No.:

17/933310

Inventors:

- Palo Alto CA, US
Siyuan Chen - Mountain View CA, US
Parker Phillips - San Francisco CA, US
Samuel Rogerson - Palo Alto CA, US

International Classification:

G06F 9/54

Abstract:

The present disclosure relates to systems and techniques for developing APIs that utilize multiple pre-existing APIs. The present disclosure also relates to a user interface that allows for chaining APIs together as a function of multiple pre-existing APIs. The present disclosure also relates to security and authorization of a user to execute one or more APIs as part of an API chain.

US Patent:

20210348220, Nov 11, 2021

Filed:

Jun 17, 2021

Appl. No.:

17/350750

Inventors:

- South San Francisco CA, US
Siyuan CHEN - San Mateo CA, US

International Classification:

C12Q 1/6837
C40B 40/06
C12N 15/10
C12Q 1/6874

Abstract:

Provided herein are compositions, methods and systems relating to libraries of polynucleotides having preselected stoichiometry with regard to species of polynucleotides such that the libraries allow for predetermined application outcomes, e.g., controlled representation after amplification and uniform enrichment after binding to target sequences. Further provided herein are polynucleotide probes and applications thereof for uniform and accurate next generation sequencing.