Sojin Shikano from 1032 Scoville Ave, Oak Park, IL 60304, age 59, Phone: 7083580550

Method To Assay Test Substances
view source
US Patent:

7883893, Feb 8, 2011
Filed:

Nov 14, 2005
Appl. No.:

11/272050
Inventors:

Min Li - Lutherville MD, US
Haiyan Sun - Baltimore MD, US
Sojin Shikano - College Park MD, US
Assignee:

The Johns Hopkins University - Baltimore MD
International Classification:

G01N 33/53
US Classification:

435375
Abstract:

Membrane proteins represent 30% of the proteome of both prokaryotes and eukaryotes. Unique to cell surface receptors is their biogenesis pathway, which involves vesicular trafficking from the endoplasmic reticulum through the Golgi apparatus and to the cell surface. Increasing evidence suggests specific regulation of biogenesis for different membrane receptors, hence affecting their surface expression. A pulse-chase assay can be used to monitor function recovery after chemobleaching (FRAC) to probe the transit time of the cell surface receptors to reach the cell surface. This method distinguishes molecular density from functional density. The ability of the reported method to access the biogenesis pathways in a high-throughput manner facilitates the identification and evaluation of molecules affecting receptor trafficking.

Biomolecule Partition Motifs And Uses Thereof
view source
US Patent:

20070031924, Feb 8, 2007
Filed:

May 15, 2006
Appl. No.:

11/435301
Inventors:

Min Li - Baltimore MD, US
Sojin Shikano - College Park MD, US
Assignee:

The Johns Hopkins University - Baltimore MD
International Classification:

C07K 14/705
C07H 21/04
C12P 21/06
US Classification:

435069100, 435320100, 435325000, 530350000, 536023500
Abstract:

The invention provides amino acid sequence motifs (e.g., biomolecule partition motifs) that can direct targeting of intracellular polypeptides to and through membranes, including cell surface membranes.

Sojin Shikano