Stephen Bengt Cheley

US Patent:

8404449, Mar 26, 2013

Filed:

Apr 24, 2009

Appl. No.:

12/429897

Inventors:

Hagan Bayley - College Station TX, US
Seong-Ho Shin - College Station TX, US
Tudor Luchian - College Station TX, US
Stephen Cheley - Bryan TX, US

Assignee:

The Texas A&M University System - College Station TX

International Classification:

G01N 33/53

US Classification:

435 71

Abstract:

A system and method for stochastic sensing in which the analyte covalently bonds to the sensor element or an adaptor element. If such bonding is irreversible, the bond may be broken by a chemical reagent. The sensor element may be a protein, such as the engineered Ptype or αHL protein pore. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable signal. Possible signals include change in electrical current, change in force, and change in fluorescence. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may be detected.

US Patent:

20030215881, Nov 20, 2003

Filed:

May 9, 2003

Appl. No.:

10/434897

Inventors:

Hagan Bayley - College Station TX, US
Seong-Ho Shin - College Station TX, US
Tudor Luchian - College Station TX, US
Stephen Cheley - Bryan TX, US

International Classification:

G01N033/53
C12M001/34
G01F001/64
G01N027/26
G01N033/50

US Classification:

435/007100, 435/287200, 205/777500

Abstract:

A system and method for stochastic sensing in which the analyte covalently bonds to the sensor element or an adaptor element. If such bonding is irreversible, the bond may be broken by a chemical reagent. The sensor element may be a protein, such as the engineered Ptype or HL protein pore. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable signal. Possible signals include change in electrical current, change in force, and change in fluorescence. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may be detected.

US Patent:

20080101988, May 1, 2008

Filed:

Dec 21, 2005

Appl. No.:

11/314174

Inventors:

Xiaofeng Kang - College Station TX, US
Li Qun Gu - Columbus MO, US
Stephen Cheley - Bryan TX, US
Hagan Bayley - Oxford, GB

Assignee:

The Texas A&M University System - College Station TX

International Classification:

G01N 21/00

US Classification:

422 58, 436518

Abstract:

The present invention includes an apparatus, system and method for stochastic sensing of an analyte to a protein pore. The protein pore may be an engineer protein pore, such as an ion channel at temperatures above 55 C. and even as high as near 100 C. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable electrical current signal. Possible signals include change in electrical current. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may also be detected.

US Patent:

20110229877, Sep 22, 2011

Filed:

Jul 6, 2009

Appl. No.:

13/002709

Inventors:

Lakmal Jayasinghe - Oxford, GB
Hagan Bayley - Oxford, GB
Stephen Cheley - East Lansing MI, US
Brian Mckeown - Chipping Norton, GB
James White - Oxford, GB
James Clarke - Oxford, GB

Assignee:

OXFORD NANOPORE TECHNOLOGIES LIMITED - Kidlington

International Classification:

C12N 9/96
C12Q 1/68
C07H 21/04

US Classification:

435 61, 435188, 536 232

Abstract:

The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

US Patent:

20140051069, Feb 20, 2014

Filed:

Aug 16, 2013

Appl. No.:

13/968778

Inventors:

- Oxford, GB
John Hagan Pryce BAYLEY - Oxford, GB
Stephen CHELEY - East Lansing MI, US
Brian MCKEOWN - Middle Barton Oxon, GB
James WHITE - Oxford, GB
James Anthony CLARKE - Oxford, GB

Assignee:

OXFORD NANOPORE TECHNOLOGIES LIMITED - Oxford

International Classification:

C12Q 1/68

US Classification:

435 61

Abstract:

The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

US Patent:

20190002972, Jan 3, 2019

Filed:

Sep 12, 2018

Appl. No.:

16/129693

Inventors:

- Oxford, GB
John Hagan Pryce Bayley - Oxford, GB
Stephen Cheley - East Lansing MI, US
Brian McKeown - Middle Barton Oxon, GB
James White - Oxford, GB
James Anthony Clarke - Oxford, GB

Assignee:

Oxford Nanopore Technologies Ltd. - Oxford

International Classification:

C12Q 1/6869
C07K 14/31
C12N 9/12
C12N 9/52
C12N 9/22
C12N 9/96
C12N 9/90
C12N 9/16

Abstract:

The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

US Patent:

20170335384, Nov 23, 2017

Filed:

Aug 3, 2017

Appl. No.:

15/667674

Inventors:

- Oxford, GB
John Hagan Pryce Bayley - Oxford, GB
Stephen Cheley - East Lansing MI, US
Brian McKeown - Middle Barton Oxon, GB
James White - Oxford, GB
James Anthony Clarke - Oxford, GB

Assignee:

Oxford Nanopore Technologies Ltd. - Oxford

International Classification:

C12Q 1/68
C12N 9/90
C12N 9/16
C12N 9/22
C07K 14/31
C12N 9/12
C12N 9/96
C12N 9/52

Abstract:

The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

US Patent:

20160076092, Mar 17, 2016

Filed:

Sep 18, 2015

Appl. No.:

14/858138

Inventors:

- Oxford, GB
John Hagan Pryce BAYLEY - Oxford, GB
Stephen CHELEY - East Lansing MI, US
Brian MCKEOWN - Middle Barton Oxon, GB
James WHITE - Oxford, GB
James Anthony CLARKE - Oxford, GB

International Classification:

C12Q 1/68

Abstract:

The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.