Stephen L Dotson

US Patent:

8450118, May 28, 2013

Filed:

Apr 28, 2010

Appl. No.:

12/799689

Inventors:

Scot Weinberger - Montara CA, US
Stephen Dotson - Montara CA, US
Nathan Harris - Morgan Hill CA, US

Assignee:

Molecular Sensing, Inc. - Montara CA

International Classification:

G01N 33/00
G01N 21/75

US Classification:

436 86, 436164, 436165, 436166, 436 63, 435 71

Abstract:

Devices and methods for detecting interaction between components associated with lipid membranes and analytes are described herein. In certain methods, a surface of a compartment of a device is coated with a material that attaches to lipid membrane from a sample. An analyte is introduced and interaction is detected, for example, by back scattering interferometry. In another method, a surface is passivated with a material that does not bind a lipid membrane. A sample is introduced that contains a membrane comprising a component for testing and an analyte. Interaction is detected. In a third method, a channel is filled with a first liquid that does not comprise a lipid membrane. A bolus of a second liquid that comprises a membrane comprising a component for testing and an analyte is introduced under laminar flow conditions so that a leading edge of the second liquid does not completely displace the first liquid in a sensing area that is interrogated by optical methods, for example, a laser. Interaction between the analyte and the component is detected.

US Patent:

20100184056, Jul 22, 2010

Filed:

Jan 8, 2010

Appl. No.:

12/655899

Inventors:

Scot Weinberger - Montara CA, US
Darryl J. Bornhop - Nashville TN, US
Stephen Dotson - Montara CA, US
Bruce J. Richardson - Los Gatos CA, US
Daniel Chang - Danville CA, US

Assignee:

Molecular Sensing, Inc. - Montara CA
Vanderbilt University - Nashville TN

International Classification:

C12Q 1/68
G01N 33/53
C12M 3/00

US Classification:

435 6, 435 71, 4352871, 4352872

Abstract:

This invention provides a device and method for collection and analysis of heterogeneous samples in a single sample container by back scattering interferometry. The sample container is configured to allow collection of a heterogeneous sample, such as blood, from a subject, separation of insoluble materials, such as blood cells by, for example, centrifugation, and mounting on a back scattering interferometer for analysis. In certain embodiments the container is a capillary tube and the interferometer comprises a mounting to hold the capillary tube in position for analysis. The device and method allow point-of-care analysis of samples.

US Patent:

20100188665, Jul 29, 2010

Filed:

Jan 8, 2010

Appl. No.:

12/655898

Inventors:

Stephen Dotson - Montara CA, US
Kirk C. Odencrantz - Eagle ID, US

Assignee:

Molecular Sensing, Inc. - Montara CA

International Classification:

G01N 21/41

US Classification:

356517

Abstract:

This invention provides methods and devices for analyzing interference patterns. The methods include fitting a Gaussian distribution to a cross correlation of two patterns from interferometric analysis of a liquid at a first and second time; identifying a positional shift of the pattern by comparing a selected value of the Gaussian distributions of the pattern at the first and second times; and determining a change in refractive index of the liquid from the positional shift. In another aspect, a method of extending the dynamic range of an interferometric data set is provided that comprises linearizing the data set, for example, using the arcsine function.