Stephen Felder

US Patent:

6458533, Oct 1, 2002

Filed:

Dec 22, 1998

Appl. No.:

09/218089

Inventors:

Stephen Felder - Tucson AZ
Bruce Seligmann - Tucson AZ
Richard M. Kris - Tucson AZ

Assignee:

High Throughput Genomics, Inc. - Tucson AZ

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912, 536232, 536 243

Abstract:

The present invention relates to compositions, apparatus and methods useful for concurrently performing multiple, high throughput, biological or chemical assays, using repeated arrays of probes. A combination of the invention comprises a surface, which comprises a plurality of test regions, at least two of which, and in a preferred embodiment, at least twenty of which, are substantially identical, wherein each of the test regions comprises an array of generic anchor molecules. The anchors are associated with bifunctional linker molecules, each containing a portion which is specific for at least one of the anchors and a portion which is a probe specific for a target of interest. The resulting array of probes is used to analyze the presence or test the activity of one or more target molecules which specifically interact with the probes. In one embodiment of the invention, the test regions (which can be wells) are further subdivided into smaller subregions (indentations, or dimples). In one embodiment of the invention, ESTs are mapped.

US Patent:

6759198, Jul 6, 2004

Filed:

Jun 26, 2001

Appl. No.:

09/888413

Inventors:

Richard M. Kris - Tucson AZ
Stephen Felder - Tucson AZ

Assignee:

High Throughout Genomics, Inc. - Tucson AZ

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912, 536 231, 536 243

Abstract:

The present invention relates to compositions, apparatus and methods useful for concurrently performing multiple, high throughput, biological or chemical assays, using repeated arrays of probes. A combination of the invention comprises a surface, which comprises a plurality of test regions, at least two of which, and in a preferred embodiment, at least twenty of which, are substantially identical, wherein each of the test regions comprises an array of generic anchor molecules. The anchors are associated with bifunctional linker molecules, each containing a portion which is specific for at least one of the anchors and a portion which is a probe specific for a target of interest. The resulting array of probes is used to analyze the presence or test the activity of one or more target molecules which specifically interact with the probes. In a preferred embodiment, a sample to be tested is subjected to a nuclease protection procedure before it is contacted with a combination of the invention.

US Patent:

7516749, Apr 14, 2009

Filed:

Apr 8, 2003

Appl. No.:

10/408108

Inventors:

Stephen Felder - Tucson AZ, US
Richard Kris - Tucson AZ, US
Brian McGraw - Tucson AZ, US

Assignee:

Neogen, LLC - Tucson AZ

International Classification:

B08B 6/00

US Classification:

134198, 436 49, 436 43

Abstract:

Methods of and apparatus for washing an array of sites in high-density microplates or similar assay plates wherein the microplates or assay plates are washed in an inverted or nearly inverted position, rather than in an upright position. Preferably, the wash liquid is dispensed upwardly in the form of a sheet from a nozzle mounted on a spray bar as the spray bar moves relative to the microplate or assay plate. After washing, the microplate or assay plate is dried with a stream of gas such as air, also preferably blown upwardly in the form of a sheet.

US Patent:

7659063, Feb 9, 2010

Filed:

Jun 14, 2004

Appl. No.:

10/865853

Inventors:

Richard Kris - Tucson AZ, US
Stephen Felder - Tucson AZ, US

Assignee:

High Throughput Genomics, Inc. - Tucson AZ

International Classification:

C12Q 1/68
C12N 1/06

US Classification:

435 6, 435259

Abstract:

The present invention relates to compositions, apparatus and methods useful for concurrently performing multiple, high throughput, biological or chemical assays, using repeated arrays of probes. A combination of the invention comprises a surface, which comprises a plurality of test regions, at least two of which, and in a preferred embodiment, at least twenty of which, are substantially identical, wherein each of the test regions comprises an array of generic anchor molecules. The anchors are associated with bifunctional linker molecules, each containing a portion which is specific for at least one of the anchors and a portion which is a probe specific for a target of interest. The resulting array of probes is used to analyze the presence or test the activity of one or more target molecules which specifically interact with the probes. In a preferred embodiment, a sample to be tested is subjected to a nuclease protection procedure before it is contacted with a combination of the invention.

US Patent:

20030039967, Feb 27, 2003

Filed:

Jun 22, 2001

Appl. No.:

09/885973

Inventors:

Richard Kris - Tucson AZ, US
Stephen Felder - Tucson AZ, US

International Classification:

C12Q001/68
C12M001/34

US Classification:

435/006000, 435/287200

Abstract:

The present invention relates to compositions, apparatus and methods useful for concurrently performing multiple, high throughput, biological or chemical assays, using repeated arrays of probes. A combination of the invention comprises a surface, which comprises a plurality of test regions, at least two of which, and in a preferred embodiment, at least twenty of which, are substantially identical, wherein each of the test regions comprises an array of generic anchor molecules. The anchors are associated with bifunctional linker molecules, each containing a portion which is specific for at least one of the anchors and a portion which is a probe specific for a target of interest. The resulting array of probes is used to analyze the presence or test the activity of one or more target molecules which specifically interact with the probes. In one embodiment of the invention, the test regions (which can be wells) are further subdivided into smaller subregions (indentations, or dimples). In one embodiment of the invention, ESTs are mapped. In another embodiment, the presence of a target nucleic acid is detected by protecting the target against nuclease digestion with a polynucleotide fragment, and analyzing the protected polynucleotide by mass spectrometry.

US Patent:

20040185464, Sep 23, 2004

Filed:

Oct 9, 2003

Appl. No.:

10/681208

Inventors:

Richard Kris - Tucson AZ, US
Stephen Felder - Tucson AZ, US

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

The present invention relates to compositions, apparatus and methods useful for concurrently performing multiple, high throughput, biological or chemical assays, using repeated arrays of probes. A combination of the invention comprises a surface, which comprises a plurality of test regions, at least two of which, and in a preferred embodiment, at least twenty of which, are substantially identical, wherein each of the test regions comprises an array of generic anchor molecules. The anchors are associated with bifunctional linker molecules, each containing a portion which is specific for at least one of the anchors and a portion which is a probe specific for a target of interest. The resulting array of probes is used to analyze the presence or test the activity of one or more target molecules which specifically interact with the probes. In one embodiment of the invention, the test regions (which can be wells) are further subdivided into smaller subregions (indentations, or dimples). In one embodiment of the invention, ESTs are mapped. In another embodiment, the presence of a target nucleic acid is detected by protecting the target against nuclease digestion with a polynucleotide fragment, and analyzing the protected polynucleotide by mass spectrometry.

US Patent:

20050239097, Oct 27, 2005

Filed:

Oct 7, 2004

Appl. No.:

10/959582

Inventors:

Richard Kris - Tucson AZ, US
Stephen Felder - Tucson AZ, US

International Classification:

C12Q001/68

US Classification:

435006000

Abstract:

A method to detect one or more nucleic acids of interest, which includes subjecting a sample that contains said nucleic acid(s) to nuclease protection with one or more protection fragments, and detecting the hybridized duplex molecules, or the single strand protected nucleic acid(s), or the protection fragment(s) with mass spectrometry.

US Patent:

20100105572, Apr 29, 2010

Filed:

Dec 1, 2008

Appl. No.:

12/325699

Inventors:

Richard M. KRIS - Tucson AZ, US
Stephen Felder - Tucson AZ, US

International Classification:

C40B 30/06

US Classification:

506 10

Abstract:

The present invention relates to compositions, apparatus and methods useful for concurrently performing multiple, high throughput, biological or chemical assays, using repeated arrays of probes. A combination of the invention comprises a surface, which comprises a plurality of test regions, at least two of which, and in a preferred embodiment, at least twenty of which, are substantially identical, wherein each of the test regions comprises an array of generic anchor molecules. The anchors are associated with bifunctional linker molecules, each containing a portion which is specific for at least one of the anchors and a portion which is a probe specific for a target of interest. The resulting array of probes is used to analyze the presence or test the activity of one or more target molecules which specifically interact with the probes. In a preferred embodiment, a sample to be tested is subjected to a nuclease protection procedure before it is contacted with a combination of the invention.