Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
STEPHEN P. HENDRICKS - LOS GATOS CA, US MICHAEL PHELAN - HAYWARD CA, US MARIAN PERIS - BELMONT CA, US CHENG-YAO CHEN - CARLSBAD CA, US DANIEL MAZUR - SAN DIEGO CA, US XINZHAN PENG - CARLSBAD CA, US AMY CASTILLO - HOUSTON TX, US
Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.
Cdna Synthesis Using A Reversibly Inactivated Reverse Transcriptase
Roland Nagel - Santa Cruz CA, US Stephen Hendricks - Los Gatos CA, US Jennifer Berkman - San Francisco CA, US Marian Peris - Belmont CA, US Yulei Wang - Foster City CA, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12P 19/34 C12N 9/10
US Classification:
435 915, 435193
Abstract:
The present invention provides compositions and methods for a reverse transcription reaction using a reversibly inactivated reverse transcriptase enzyme. The reversibly inactivated reverse transcriptase enzyme results from a chemical modification which inactivates the reverse transcriptase enzyme. The activity of the reverse transcriptase enzyme is recovered by an incubation of the reaction mixture at elevated temperature prior to, or as part of the reverse transcription reaction. The reverse transcriptase enzyme of the present invention provides for a signficant reduction in non-specific reverse transcription from template nucleic acid molecules because the formulation of the reaction mixture does not support the formation of reverse transcription products prior to activation of the reverse transcriptase.
Lei Xi - Foster City CA, US Keith P. Bjornson - Union City CA, US Stephen P. Hendricks - Los Gatos CA, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12Q 1/68 C07H 21/00
US Classification:
435 612, 536 2433
Abstract:
This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.
Workflow For Detection Of Ligands Using Nucleic Acids
Shiaw-Min Chen - Fremont CA, US Elana Swartzman - Alameda CA, US David Ruff - San Francisco CA, US Mark Shannon - San Francisco CA, US Julia Lu - , US Stephen Hendricks - Los Gatos CA, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12Q 1/68
US Classification:
435 612
Abstract:
This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.
Polymerization Of Nucleic Acids Using Activation By Polyphosphorolysis (App) Reactions
Lei (Larry) XI - Foster City CA, US Paul Kenney - Sunnyvale CA, US Zhaochun Ma - Sunnyvale CA, US Dennis Prosen - Foster City CA, US Stephen Hendricks - Los Gatos CA, US Roland Nagel - Santa Cruz CA, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12P 19/34
US Classification:
435 912, 435 915, 435 9151
Abstract:
This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.
Stephen P. HENDRICKS - Los Gatos CA, US Michael PHELAN - Hayward CA, US Marian PERIS - Belmont CA, US Cheng-Yao CHEN - Carlsbad CA, US Daniel MAZUR - San Diego CA, US Xinzhan PENG - Carlsbad CA, US Amy CASTILLO - Houston TX, US
Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.
Marian PERIS - Belmont CA, US Michael PHELAN - Hayward CA, US Barnett B. ROSENBLUM - San Jose CA, US Stephen P. HENDRICKS - Los Gatos CA, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C12N 9/12
US Classification:
435194
Abstract:
Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
License Records
Stephen C Hendricks
License #:
306051-6303 - Expired
Category:
Security Companies & Guards
Issued Date:
Nov 25, 1992
Expiration Date:
Nov 29, 1993
Type:
Unarmed Private Security Officer
Stephen E. Hendricks
License #:
E-2809 - Expired
Category:
Engineering Intern
Googleplus
Stephen Hendricks
Lived:
Seattle Oklahoma City Sarasota, FL Princeton, New Jersey Berkeley, California Austin, Texas Ann Arbor, MI Ithaca, New York
University of Michigan - Political Science, Cornell University - Political Science, New College of Florida - Intellectual History, Putnam City High School - Oklahoma City