Stephen M Hughes

US Patent:

8513214, Aug 20, 2013

Filed:

Nov 10, 2008

Appl. No.:

12/741873

Inventors:

Maqbool A. Siddiqui - Silver Spring MD, US
Victor E. Marquez - Montgomery Village MD, US
Stephen H. Hughes - Smithsburg MD, US
Paul L. Boyer - Greencastle PA, US

Assignee:

The United States of America as Represented by the Secretary of the Dept. of Health and Human Services - Washington DC

International Classification:

A01N 43/04
A61K 31/70
C07H 19/16
C07H 19/167
C07H 19/173
C12Q 1/70

US Classification:

514 45, 514 46, 435 5, 536 2721, 536 276

Abstract:

The invention provides for novel 2-Deoxyadenosine compounds, which can treat HIV infection at low cytotoxicity values. Substitution at the 4′-position provided compounds which demonstrated low cytotoxicity values in an ATP-based cytotoxicity assay.

US Patent:

20140273137, Sep 18, 2014

Filed:

Mar 15, 2013

Appl. No.:

13/837654

Inventors:

The Unites Stated of America, as represented by the Secretary of Agriculture - , US
Dwayne R. Roach - Laurel MD, US
Piyum A. Khatibi - Dunlap IL, US
Kenneth M. Bischoff - Morton IL, US
Stephen R. Hughes - Peoria IL, US

Assignee:

The United States of America, as represented by the Secretary of Agriculture - Washington DC

International Classification:

C12P 7/10
C12P 7/06

US Classification:

435165, 435161, 435163

Abstract:

Ethanol losses due to bacterial contamination in fermentation cultures weakens the economics of biofuel production. species are the predominant contaminant. Bacteriophage lytic enzymes are peptidoglycan hydrolases which degrade Gram positive cell walls when exposed externally and are a novel source of antimicrobials. The streptococcal phage λSA2 endolysin construct demonstrated strong lytic activity towards 17 of 22 strains of lactobacilli, staphylococci or streptococci maintaining optimal specific activity under fermentation conditions toward substrates. bacteriophage endolysin constructs LysA, LysA2 and LysgaY showed exolytic activity towards 60% of the lactobacilli tested including four isolates from fuel ethanol fermentations. Presence of ethanol (≦5%) did not affect lytic activity. Lysins were able to reduce both and contaminants in mock fermentations of corn fiber hydrolysates. Recombinant LysA and λSa2 expressed in the yeast are functional; LysA was shown to reduce lactobacilli in experimentally infected fermentations.