Stephen R Lane

US Patent:

6480730, Nov 12, 2002

Filed:

Dec 20, 2000

Appl. No.:

09/742775

Inventors:

Christopher B. Darrow - Pleasanton CA
Stephen M. Lane - Oakland CA
Abraham P. Lee - Walnut Creek CA
Amy W. Wang - Berkeley CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

A61B 505

US Classification:

600347, 600345

Abstract:

An implantable chemical sensor system for medical applications is described which permits selective recognition of an analyte using an expandable biocompatible sensor, such as a polymer, that undergoes a dimensional change in the presence of the analyte. The expandable polymer is incorporated into an electronic circuit component that changes its properties (e. g. , frequency) when the polymer changes dimension. As the circuit changes its characteristics, an external interrogator transmits a signal transdermally to the transducer, and the concentration of the analyte is determined from the measured changes in the circuit. This invention may be used for minimally invasive monitoring of blood glucose levels in diabetic patients.

US Patent:

6766183, Jul 20, 2004

Filed:

Dec 28, 2001

Appl. No.:

10/033240

Inventors:

Joseph C. Walsh - Los Angeles CA
Aaron M. Heiss - Orange OH
Glenn Noronha - Oceanside CA
David J. Vachon - Granada Hills CA
Stephen M. Lane - Oakland CA
Thomas A. Peyser - Menlo Park CA
William Peter Van Antwerp - Valencia CA
John Joseph Mastrototaro - Los Angeles CA

Assignee:

Medtronic MiniMed, Inc. - Northridge CA
The Regents of the University of California - Oakland CA

International Classification:

A61B 500

US Classification:

600317, 600341, 436 94, 436 95, 436172, 422 8207, 568 1, 546 13

Abstract:

Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. Embodiments of these biosensor molecules exhibit fluorescence emission at wavelengths greater than about 650 nm. Typical biosensor molecules include a fluorophore that includes an iminium ion, a linker moiety that includes a group that is an anilinic type of relationship to the fluorophore and a boronate substrate recognition/binding moiety, which binds glucose. The fluorescence molecules modulated by the presence or absence of polyhydroxylated analytes such as glucose. This property of these molecules of the invention, as well as their ability to emit fluorescent light at greater than about 650 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.

US Patent:

6818964, Nov 16, 2004

Filed:

Sep 30, 2002

Appl. No.:

10/261349

Inventors:

Michael P. Surh - Livermore CA
William D. Wilson - Pleasanton CA
Stephen M. Lane - Oakland CA

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

H01L 2900

US Classification:

257531, 257724

Abstract:

Nanochannel electrophoretic and electrochemical devices having selectively-etched nanolaminates located in the fluid transport channel. The normally flat surfaces of the nanolaminate having exposed conductive (metal) stripes are selectively-etched to form trenches and baffles. The modifications of the prior utilized flat exposed surfaces increase the amount of exposed metal to facilitate electrochemical redox reaction or control the exposure of the metal surfaces to analytes of large size. These etched areas variously increase the sensitivity of electrochemical detection devices to low concentrations of analyte, improve the plug flow characteristic of the channel, and allow additional discrimination of the colloidal particles during cyclic voltammetry.

US Patent:

7067351, Jun 27, 2006

Filed:

Jun 8, 2004

Appl. No.:

10/864778

Inventors:

Michael P. Surh - Livermore CA, US
William D. Wilson - Pleasanton CA, US
Stephen M. Lane - Oakland CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

H01L 21/44

US Classification:

438107, 438245, 438607, 257E21586, 257751, 257499, 427250, 427252, 42725515, 42725523, 42725526, 42725528, 4272557

Abstract:

Nanochannel electrophoretic and electrochemical devices having selectively-etched nanolaminates located in the fluid transport channel. The normally flat surfaces of the nanolaminate having exposed conductive (metal) stripes are selectively-etched to form trenches and baffles. The modifications of the prior utilized flat exposed surfaces increase the amount of exposed metal to facilitate electrochemical redox reaction or control the exposure of the metal surfaces to analytes of large size. These etched areas variously increase the sensitivity of electrochemical detection devices to low concentrations of analyte, improve the plug flow characteristic of the channel, and allow additional discrimination of the colloidal particles during cyclic voltammetry.

US Patent:

7076092, Jul 11, 2006

Filed:

Jun 12, 2002

Appl. No.:

10/170876

Inventors:

Christopher W. Hollars - Brentwood CA, US
Thomas R. Huser - Livermore CA, US
Stephen M. Lane - Oakland CA, US
Rodney L. Balhorn - Livermore CA, US
Olgica Bakajin - San Leandro CA, US
Christopher Darrow - Pleasanton CA, US

Assignee:

The United States of America as represented by the United States Department of Energy - Washington DC

International Classification:

G06K 9/00

US Classification:

382133

Abstract:

A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0. 5 μm×100 μm×100 μm. A device using these methods would have 10–10greater throughput than previous one-dimensional (1D) micro-stream devices having 1 μminterrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.

US Patent:

7118661, Oct 10, 2006

Filed:

Sep 30, 2002

Appl. No.:

10/261392

Inventors:

Michael P. Surh - Livermore CA, US
William D. Wilson - Pleasanton CA, US
Stephen M. Lane - Oakland CA, US

Assignee:

The Regents of the University of California - Oakland CA

International Classification:

G01N 27/453

US Classification:

204601, 204643, 422100

Abstract:

A microfluidic device made from nanolaminate materials that are capable of electrophoretic selection of particles on the basis of their mobility. Nanolaminate materials are generally alternating layers of two materials (one conducting, one insulating) that are made by sputter coating a flat substrate with a large number of layers. Specific subsets of the conducting layers are coupled together to form a single, extended electrode, interleaved with other similar electrodes. Thereby, the subsets of conducting layers may be dynamically charged to create time-dependent potential fields that can trap or transport charge colloidal particles. The addition of time-dependence is applicable to all geometries of nanolaminate electrophoretic and electrochemical designs from sinusoidal to nearly step-like.

US Patent:

7301624, Nov 27, 2007

Filed:

Sep 7, 2004

Appl. No.:

10/935783

Inventors:

Chad E. Talley - Brentwood CA, US
Thomas R. Huser - Livermore CA, US
Christopher W. Hollars - Brentwood CA, US
Stephen M. Lane - Oakland CA, US
Bradley R. Hart - Brentwood CA, US
Ted A. Laurence - Livermore CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

G01J 3/44

US Classification:

356301

Abstract:

Surface-Enhanced Raman Spectroscopy (SERS) is a vibrational spectroscopic technique that utilizes metal surfaces to provide enhanced signals of several orders of magnitude. When molecules of interest are attached to designed metal nanoparticles, a SERS signal is attainable with single molecule detection limits. This provides an ultrasensitive means of detecting the presence of molecules. By using selective chemistries, metal nanoparticles can be functionalized to provide a unique signal upon analyte binding. Moreover, by using measurement techniques, such as, ratiometric received SERS spectra, such metal nanoparticles can be used to monitor dynamic processes in addition to static binding events. Accordingly, such nanoparticles can be used as nanosensors for a wide range of chemicals in fluid, gaseous and solid form, environmental sensors for pH, ion concentration, temperature, etc. , and biological sensors for proteins, DNA, RNA, etc.

US Patent:

20020043651, Apr 18, 2002

Filed:

Apr 4, 2001

Appl. No.:

09/826745

Inventors:

Christopher Darrow - Pleasanton CA, US
Joe Satcher - Patterson CA, US
Stephen Lane - Oakland CA, US
Jennifer Gable - Livermore CA, US

International Classification:

G01N031/00

US Classification:

252/408100

Abstract:

The invention disclosed herein provides fluorescence based methods for the determination of polyhydroxylated analyte concentrations as well as optical polyhydroxylate analyte sensors and sensor systems. In particular, the invention provides methods of quantifying the abundances or concentrations of polyhydroxylate analyte by measuring changes in the fluorescence lifetimes. The methods of the invention are based on the observation that fluorescent sensor molecules capable of binding a polyhydroxylated analyte such as glucose have distinct fluorescent lifetimes depending upon whether they are in a form that is either bound to analyte or a form that is not bound to the analyte. The distinct and measurable differences in the fluorescence lifetimes of the different fluorescent sensor species can be used to determine the relative abundance of the bound and unbound fluorescent sensor species, a parameter which can then be correlated to the concentration of the analyte.