Stephen Ernest Lane

US Patent:

6766183, Jul 20, 2004

Filed:

Dec 28, 2001

Appl. No.:

10/033240

Inventors:

Joseph C. Walsh - Los Angeles CA
Aaron M. Heiss - Orange OH
Glenn Noronha - Oceanside CA
David J. Vachon - Granada Hills CA
Stephen M. Lane - Oakland CA
Thomas A. Peyser - Menlo Park CA
William Peter Van Antwerp - Valencia CA
John Joseph Mastrototaro - Los Angeles CA

Assignee:

Medtronic MiniMed, Inc. - Northridge CA
The Regents of the University of California - Oakland CA

International Classification:

A61B 500

US Classification:

600317, 600341, 436 94, 436 95, 436172, 422 8207, 568 1, 546 13

Abstract:

Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. Embodiments of these biosensor molecules exhibit fluorescence emission at wavelengths greater than about 650 nm. Typical biosensor molecules include a fluorophore that includes an iminium ion, a linker moiety that includes a group that is an anilinic type of relationship to the fluorophore and a boronate substrate recognition/binding moiety, which binds glucose. The fluorescence molecules modulated by the presence or absence of polyhydroxylated analytes such as glucose. This property of these molecules of the invention, as well as their ability to emit fluorescent light at greater than about 650 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.

US Patent:

7076092, Jul 11, 2006

Filed:

Jun 12, 2002

Appl. No.:

10/170876

Inventors:

Christopher W. Hollars - Brentwood CA, US
Thomas R. Huser - Livermore CA, US
Stephen M. Lane - Oakland CA, US
Rodney L. Balhorn - Livermore CA, US
Olgica Bakajin - San Leandro CA, US
Christopher Darrow - Pleasanton CA, US

Assignee:

The United States of America as represented by the United States Department of Energy - Washington DC

International Classification:

G06K 9/00

US Classification:

382133

Abstract:

A method and apparatus with the sensitivity to detect and identify single target molecules through the localization of dual, fluorescently labeled probe molecules. This can be accomplished through specific attachment of the taget to a surface or in a two-dimensional (2D) flowing fluid sheet having approximate dimensions of 0. 5 μm×100 μm×100 μm. A device using these methods would have 10–10greater throughput than previous one-dimensional (1D) micro-stream devices having 1 μminterrogation volumes and would for the first time allow immuno- and DNA assays at ultra-low (femtomolar) concentrations to be performed in short time periods (10 minutes). The use of novel labels (such as metal or semiconductor nanoparticles) may be incorporated to further extend the sensitivity possibly into the attomolar range.

US Patent:

7301624, Nov 27, 2007

Filed:

Sep 7, 2004

Appl. No.:

10/935783

Inventors:

Chad E. Talley - Brentwood CA, US
Thomas R. Huser - Livermore CA, US
Christopher W. Hollars - Brentwood CA, US
Stephen M. Lane - Oakland CA, US
Bradley R. Hart - Brentwood CA, US
Ted A. Laurence - Livermore CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

G01J 3/44

US Classification:

356301

Abstract:

Surface-Enhanced Raman Spectroscopy (SERS) is a vibrational spectroscopic technique that utilizes metal surfaces to provide enhanced signals of several orders of magnitude. When molecules of interest are attached to designed metal nanoparticles, a SERS signal is attainable with single molecule detection limits. This provides an ultrasensitive means of detecting the presence of molecules. By using selective chemistries, metal nanoparticles can be functionalized to provide a unique signal upon analyte binding. Moreover, by using measurement techniques, such as, ratiometric received SERS spectra, such metal nanoparticles can be used to monitor dynamic processes in addition to static binding events. Accordingly, such nanoparticles can be used as nanosensors for a wide range of chemicals in fluid, gaseous and solid form, environmental sensors for pH, ion concentration, temperature, etc. , and biological sensors for proteins, DNA, RNA, etc.

US Patent:

20080239307, Oct 2, 2008

Filed:

Mar 30, 2007

Appl. No.:

11/731418

Inventors:

Chad E. Talley - Tustin CA, US
Thomas R. Huser - El Grove CA, US
Christopher W. Hollars - Overland Park KS, US
Stephen M. Lane - Oakland CA, US

International Classification:

G01J 3/44

US Classification:

356301

Abstract:

A surface-enhanced Raman scattering method and apparatus to sequence polymeric biomolecules such as DNA, RNA, or proteins is introduced. The method uses metallic nanostructures such as, for example, spherical or cylindrical Au or Ag nanoparticles having characteristic lengths of 10-100 nm which when illuminated with light of the appropriate wavelength produce resonant oscillations of the conduction electrons (plasmon resonance). Electric field enhancements of 30-1000 near the particle surface resulting from such oscillations increase Raman scattering cross-sections by about 10-10due to the Edependence of the Raman scattering, wherein the largest enhancements occur in the gap/junction between novel closely spaced structures as disclosed herein.