Stephen L Mayfield

US Patent:

RE39350, Oct 17, 2006

Filed:

Jan 16, 1998

Appl. No.:

10/310587

Inventors:

Stephen P. Mayfield - Cardiff CA, US

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

C12Q 1/68

US Classification:

435 6, 4353201, 435419, 435 691, 435375, 435468, 536 231

Abstract:

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

US Patent:

7678561, Mar 16, 2010

Filed:

May 9, 2007

Appl. No.:

11/801506

Inventors:

Stephen P. Mayfield - Cardiff CA, US

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

C12N 15/82

US Classification:

4352572, 800278, 800288, 4352576, 435468, 435 711

Abstract:

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery or proteins/peptides, especially gut active proteins, without purification is disclosed.

US Patent:

RE44266, Jun 4, 2013

Filed:

Jan 16, 1998

Appl. No.:

11/197730

Inventors:

Stephen P. Mayfield - Cardiff CA, US

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

C12P 21/02

US Classification:

435 691, 4353201, 435419, 435375, 435468, 536 231

Abstract:

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

US Patent:

20040014174, Jan 22, 2004

Filed:

Apr 23, 2003

Appl. No.:

10/422628

Inventors:

Stephen Mayfield - Cardiff CA, US
Scott Franklin - Cardiff CA, US

International Classification:

C12P021/02
C07H021/04
C12N005/04
C07K016/00

US Classification:

435/069100, 435/320100, 435/419000, 530/387100, 536/023500

Abstract:

Methods of producing one or more polypeptides in a plant chloroplast, including methods of producing polypeptides that specifically associate in a plant chloroplast to generate a functional protein complex, are provided. An isolated polynucleotide that includes (or encodes) a first ribosome binding sequence (RBS) operatively linked to a second RBS, such that the first RBS directs translation of a polypeptide in a prokaryote and the second RBS directs translation of the polypeptide in a chloroplast, also is provided, as is a vector containing such a polynucleotide, particularly a chloroplast vector and a chloroplast/prokaryote shuttle vector. Also provided is a synthetic polynucleotide, which is chloroplast codon biased. A plant cell that is genetically modified to contain a polynucleotide or vector as described above, as well as transgenic plants containing or derived from such a genetically modified cell, are provide. Polypeptides encoded by a synthetic polynucleotide as described also are provided.

US Patent:

20100129394, May 27, 2010

Filed:

Jan 27, 2010

Appl. No.:

12/695101

Inventors:

Stephen P. Mayfield - Cardiff CA, US

International Classification:

A61K 36/02
C12N 15/63

US Classification:

42419517, 4353201

Abstract:

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.

US Patent:

20120309939, Dec 6, 2012

Filed:

Nov 19, 2010

Appl. No.:

13/510229

Inventors:

Beth A. Rasala - San Diego CA, US
Rosa M. F. Cardoso - San Diego CA, US
Machiko Muto - San Diego CA, US
Stephen P. Mayfield - Cardiff by the Sea CA, US
Philip A. Lee - San Diego CA, US
Craig A. Behnke - San Diego CA, US
Michael Mendez - San Diego CA, US

Assignee:

THE SCRIPPS RESEARCH INSTITUTE - La Jolla CA
SAPPHIRE ENERGY, INC. - San Diego CA

International Classification:

C12P 21/00
C07K 14/00
C12N 1/13

US Classification:

530350, 4352572, 435 691

Abstract:

The present disclosure relates to methods of expressing therapeutic proteins in photosynthetic organisms and the therapeutic proteins produced by the methods. The therapeutic proteins include high-mobility group box 1 (HMGB1) protein, fibronectin domain (10) (10FN3), fibronectin domain (14) (14FN3), interferon beta (IFNβ), proinsulin and vascular endothelial growth factor (VEGF). The photosynthetic organisms include prokaryotes such as cyanobacteria and eukaryotes such as alga and plants. Transformation of eukaryotes is preferably the plastid genome, more preferably the chloroplast genome.

US Patent:

62946536, Sep 25, 2001

Filed:

Jul 10, 2000

Appl. No.:

9/613182

Inventors:

Stephen Mayfield - Cardiff CA

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

C07K 14415

US Classification:

530350

Abstract:

The present invention relates to recombinant protein disulfide isomerase, RB60, that functions as a translational regulator of the binding of a translational activator protein, RB47, to its binding site for the activation of translation. The recombinant RB60 protein is useful in a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants.

US Patent:

61565172, Dec 5, 2000

Filed:

Jul 13, 1999

Appl. No.:

9/341550

Inventors:

Stephen Mayfield - Cardiff CA

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.