Stephen A Mordue

US Patent:

8222023, Jul 17, 2012

Filed:

Sep 10, 2008

Appl. No.:

12/207627

Inventors:

C. Frederick Battrell - Redmond WA, US
John Gerdes - Columbine Valley CO, US
John R. Williford - Sammamish WA, US
Denise Maxine Hoekstra - Monroe WA, US
Wayne L. Breidford - Seattle WA, US
Stephen Mordue - Kirkland WA, US
John Clemmens - Redmond WA, US
Melud Nabavi - Seattle WA, US
Mark Kokoris - Bothell WA, US

Assignee:

Micronics, Inc. - Redmond WA

International Classification:

C12M 1/34

US Classification:

4352872, 4352885, 4352865

Abstract:

Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.

US Patent:

20060246575, Nov 2, 2006

Filed:

Jan 13, 2006

Appl. No.:

11/331587

Inventors:

Christy Lancaster - Seattle WA, US
C. Battrell - Redmond WA, US
Jason Capodanno - Redmond WA, US
John Gerdes - Columbine Valley CO, US
Mark Kokoris - Bothell WA, US
Melud Nabavi - Seattle WA, US
Stephen Mordue - Seattle WA, US
Robert McRuer - Mercer Island WA, US
John Clemmens - Redmond WA, US
Wayne Breidford - Seattle WA, US

Assignee:

Micronics, Inc. - Redmond WA

International Classification:

C12M 1/34

US Classification:

435287200, 435288500

Abstract:

The present invention relates to microfluidic devices and methods for detecting rare cells. The disclosed microfluidic devices and methods integrate and automate sample preparation, cell labeling, cell sorting and enrichment, and DNA/RNA analysis of sorted cells.

US Patent:

20090148847, Jun 11, 2009

Filed:

Sep 3, 2008

Appl. No.:

12/203779

Inventors:

Mark Kokoris - Bothell WA, US
Melud Nabavi - Seattle WA, US
Wayne L. Breidford - Seattle WA, US
John Gerdes - Columbine Valley CO, US
Stephen Mordue - Kirkland WA, US
C. Frederick Battrell - Redmond WA, US

Assignee:

MICRONICS, INC. - Redmond WA

International Classification:

C12Q 1/68
C07H 21/04
B01J 19/00

US Classification:

435 6, 536 2433, 42218601

Abstract:

Disclosed is an improvement in methods for nucleic acid and immunological bioassays. The methods comprise a step for “sweeping” paramagnetic bead: target molecule complexes so as to capture them with an affinity capture agent on a test pad by moving a magnetic force field from outside to inside the test pad area so as to bring into contact the paramagnetic complexes with the capture agent, while sweeping any unbound paramagnetic material off the test pad by moving the magnetic field from inside to outside the test pad area. Surprisingly, the paramagnetic complexes are rapidly affinity-extracted from the moving magnetic field.

US Patent:

20090181411, Jul 16, 2009

Filed:

Dec 22, 2008

Appl. No.:

12/341637

Inventors:

C. Frederick Battrell - Redmond WA, US
John Gerdes - Columbine Valley CO, US
Stephen Mordue - Kirkland WA, US
Jason Capodanno - Redmond WA, US
Denise Maxine Hoekstra - Monroe WA, US
John R. Williford - Sammamish WA, US
John Clemmens - Redmond WA, US

Assignee:

Micronics, Inc. - Redmond WA

International Classification:

G01N 33/53
B01J 19/00
C12M 1/00
G01N 21/00
G01N 21/64
G01N 33/00

US Classification:

435 792, 422 681, 4352872, 436164, 436172, 422 73

Abstract:

Microfluidic methods and devices for heterogeneous binding and agglutination assays are disclosed, with improvements relating to mixing and to reagent and sample manipulation in systems designed for safe handling of clinical test samples.

US Patent:

20090325276, Dec 31, 2009

Filed:

Mar 25, 2009

Appl. No.:

12/411020

Inventors:

C. Frederick Battrell - Redmond WA, US
John Gerdes - Columbine Valley CO, US
Wayne L. Breidford - Seattle WA, US
Jason Capodanno - Redmond WA, US
Stephen Mordue - Los Gatos CA, US
John Clemmens - Redmond WA, US
Denise Maxine Hoekstra - Monroe WA, US
Christy A. Lancaster - Seattle WA, US
John R. Williford - Sammamish WA, US
Patrick Maloney - Bothell WA, US
Joan Haab - Seattle WA, US

Assignee:

Micronics, Inc. - Redmond WA

International Classification:

C12M 1/00

US Classification:

4352872

Abstract:

Combinations of microfluidic diagnostic testing modules for simultaneous evaluations of serological and molecular biological targets are provided, and include panel testing for both antibodies (or antigens) and nucleic acid targets in one single-use device. These improvements are directed to evaluating the overall progress and activity of a pathogenic process in real time, at the point of care, not merely the presence or absence of a particular diagnostic marker, which can often be incomplete or misleading.

US Patent:

20120164627, Jun 28, 2012

Filed:

Jan 3, 2012

Appl. No.:

13/342859

Inventors:

C. Frederick Battrell - Redmond WA, US
John Gerdes - Columbine Valley CO, US
Stephen Mordue - Kirkland WA, US
Jason Capodanno - Redmond WA, US
Denise Maxine Hoekstra - Monroe WA, US
John R. Williford - Sammamish WA, US
John Clemmens - Redmond WA, US

Assignee:

Micronics, Inc. - Redmond WA

International Classification:

G01N 33/53
G01N 33/569
G01N 21/75
B01L 3/00
C12M 1/40

US Classification:

435 5, 422505, 436501, 435 792, 435 795, 4352872, 422 69

Abstract:

Microfluidic methods and devices for heterogeneous binding and agglutination assays are disclosed, with improvements relating to mixing and to reagent and sample manipulation in systems designed for safe handling of clinical test samples.

US Patent:

20120329142, Dec 27, 2012

Filed:

Jun 8, 2012

Appl. No.:

13/492612

Inventors:

C. Frederick BATTRELL - Redmond WA, US
John GERDES - Columbine Valley CO, US
John R. WILLIFORD - Sammamish WA, US
Denise Maxine HOEKSTRA - Monroe WA, US
Wayne L. BREIDFORD - Seattle WA, US
Stephen MORDUE - Kirkland WA, US
John CLEMMENS - Redmond WA, US
Melud NABAVI - Seattle WA, US
Mark KOKORIS - Bothell WA, US

Assignee:

MICRONICS, INC. - Redmond WA

International Classification:

C12M 1/34

US Classification:

4352872

Abstract:

Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed.

US Patent:

20150158026, Jun 11, 2015

Filed:

Jan 30, 2015

Appl. No.:

14/610493

Inventors:

- Redmond WA, US
John Gerdes - Columbine Valley CO, US
Stephen Mordue - Los Gatos CA, US
Jason Capodanno - Redmond WA, US
Denise Maxine Hoekstra - Monroe WA, US
John R. Williford - Sammamish WA, US
John Clemmens - Redmond WA, US

International Classification:

B01L 3/00
G01N 33/543

Abstract:

Microfluidic methods and devices for heterogeneous binding and agglutination assays are disclosed, with improvements relating to mixing and to reagent and sample manipulation in systems designed for safe handling of clinical test samples.