Steve J Chang

US Patent:

6461874, Oct 8, 2002

Filed:

Jan 26, 2000

Appl. No.:

09/491139

Inventors:

Wei-Chao Ni - Foxboro MA
Daniel W. Eustace - North Attleboro MA
Steve Chin-Shen Chang - Franklin MA

Assignee:

Bayer Corporation - East Walpole MA

International Classification:

G01N 33553

US Classification:

436526, 436531, 436534, 436810, 436548, 435 79, 435188, 435963, 435176, 435180, 427414, 427337, 427338, 427222, 427 2, 428403, 428407, 530815

Abstract:

It has been found that casein and salts of casein are useful as replacements for, or in addition to, BSA as materials for coating solid phases, particularly magnetic particles, used in immunoassays and other binding assays for separation of the desired analyte. By using casein, immunoassays having improved stability and fewer discordant samples have been developed. Casein used at a concentration of 0. 05-4. 0 grams per gram of paramagnetic particle (optimally approximately 0. 78-1. 2 grams of casein per gram of magnetic particle) has been found to confer this benefit.

US Patent:

6756234, Jun 29, 2004

Filed:

Dec 21, 2001

Appl. No.:

10/029541

Inventors:

Wei-Chao Ni - Foxboro MA
Daniel W. Eustace - North Attleboro MA
Steve Chin-Shen Chang - Franklin MA

Assignee:

Bayer Corporation - Tarrytown NY

International Classification:

G01N 33553

US Classification:

436526, 436531, 436534, 436810, 436548, 435 79, 435188, 435963, 435176, 435180, 427414, 427337, 427338, 427222, 427 2, 428403, 428407, 530815

Abstract:

It has been found that casein and salts of casein are useful as replacements for, or in addition to, BSA as materials for coating solid phases, particularly magnetic particles, used for immunoassays and other binding assays for separation of the desired analyte. By using casein, immunoassays having improved stability and few discordant samples have been developed. Casein used at a concentration of 0. 05-4. 0 grams per gram of paramagnetic particle (optimally approximately 0. 78-1. 2 grams of casein per gram of magnetic particle) has been found to confer this benefit. In addition, a process for coating solid phases has been invented, said process comprising the mixing of casein with magnetic particles at 30-60Â C. for 5-180 hours, said process resulting in casein-coated paramagnetic particles which either (1) already have combined therewith active ingredients needed in the binding assay or (2) are capable of reacting with active ingredients needed in the binding assay. Furthermore, a process for using the casein-coated paramagnetic particles have been developed, said particles being directly or indirectly combined with active ingredients used in the immunoassays.