Steve J Lackie

US Patent:

6664114, Dec 16, 2003

Filed:

Jul 18, 1994

Appl. No.:

08/277225

Inventors:

Steve J. Lackie - Boise ID
Thomas R. Glass - Idaho City ID

Assignee:

Sapidyne Instruments, Inc. - Boise ID

International Classification:

G01N 33543

US Classification:

436518, 435 71, 435 792, 435 795, 435962, 435967, 436514, 436523, 436538, 422 55, 422 56, 422 57

Abstract:

The present invention provides an improved system for detecting the presence or level of an analyte in a sample. In âcompetition-likeâ assays of the present invention, a sample including an analyte is mixed with a second ligand to which the analyte binds, and the mixture is exposed to a solid phase containing a first ligand that can compete with the analyte for binding to the second ligand. According to the present invention, the time of exposure of the mixture to the solid phase is limited so that substantially no dissociation of analyte/second ligand complex occurs. The competition-like assays of the present invention are preferably performed with a solid phase containing a substantial excess of first ligand. In âsandwich-typeâ assays of the present invention, a sample including an analyte is contacted with a solid phase including a first ligand that binds the analyte and, simultaneously or subsequently, is contacted with a second ligand that binds the analyte (or the analyte/first ligand complex). The time of contact between the second ligand and the solid phase is limited so that substantially no non-specific binding between the second ligand and the solid phase occurs.

US Patent:

7091050, Aug 15, 2006

Filed:

Oct 22, 2003

Appl. No.:

10/690711

Inventors:

Steve J. Lackie - Boise ID, US
Thomas R. Glass - Idaho City ID, US

Assignee:

Sapidyne Instruments Inc. - Bosie ID

International Classification:

G01N 33/543

US Classification:

436518, 435 71, 435 792, 435 793, 435 795, 435962, 435967, 436514, 436523, 436538

Abstract:

The present invention provides an improved system for detecting the presence or level of an analyte in a sample. In “competition-like” assays of the present invention, a sample including an analyte is mixed with a second ligand to which the analyte binds, and the mixture is exposed to a solid phase containing a first ligand that can compete with the analyte for binding to the second ligand. According to the present invention, the time of exposure of the mixture to the solid phase is limited so that substantially no dissociation of analyte/second ligand complex occurs. The competition-like assays of the present invention are preferably performed with a solid phase containing a substantial excess of first ligand. In “sandwich-type” assays of the present invention, a sample including an analyte is contacted with a solid phase including a first ligand that binds the analyte and, simultaneously or subsequently, is contacted with a second ligand that binds the analyte (or the analyte/first ligand complex). The time of contact between the second ligand and the solid phase is limited so that substantially no non-specific binding between the second ligand and the solid phase occurs.

US Patent:

20160195518, Jul 7, 2016

Filed:

Jan 7, 2015

Appl. No.:

14/591624

Inventors:

- Boise ID, US
Steve J. Lackie - Boise ID, US

International Classification:

G01N 33/53

Abstract:

The present disclosure is directed toward improved methods of conducting a competitive binding assay or experiment. The methodology includes utilizing either a positive control, a negative control, or both in order to scale experimentation results to results that can be utilized to obtain a precise measurement of the kinetic rate constant (the off rate denoted as k) describing the dissociation of a non-covalent complex such as an antibody antigen complex, receptor ligand complex, etc.