Steven W Andersen

US Patent:

52905535, Mar 1, 1994

Filed:

Jul 22, 1991

Appl. No.:

7/733021

Inventors:

Maurice M. Iwu - Silver Spring MD
Daniel L. Klayman - Chevy Chase MD
Joan E. Jackson - Rockville MD
John D. Tally - Washington DC
Steven L. Andersen - Silver Spring MD

Assignee:

The United States of America as represented by the Secretary of the Army - Washington DC

International Classification:

A61K 3578
A61K 3144

US Classification:

4241951

Abstract:

A method of preparing substantially purified alkaloids from seeds, stems, uit-rind and bark of a plant selected from Picralima nitida, Gongronema latifolia, Dorstenia multiradiata, Cola attiensis, Rothmania withfieldii and Desmodium gangeticum, for use in the treatment of protozoal diseases, comprising: pulverizing said plant; a first solvent, drying the extracted material and re-extracting the dried material with a different solvent; extracting a fresh sample of said plant with boiling water; filtering and concentrating the boiling water solvent extracts under reduced pressure; concentrating the dried extract to a gum and re-extracting said gum with an aqueous acidic HCl solution; filtering the acidic extract and making it alkaline to a pH of about 9 with a concentration NaOH solution; extracting the alkaline solution with dichloromethane; concentrating organic layers of the extracted alkaline solution to dryness under reduced pressure to obtain an alkaloid fraction; and separating the alkaloid fraction by liquid chromotography-mass spectrometry to obtain substantially purified alkaloids for use in treatment of protozoal diseases.