Steven W Bott

US Patent:

58719466, Feb 16, 1999

Filed:

May 18, 1995

Appl. No.:

8/444056

Inventors:

Frank J. Lucas - Boca Raton FL
Gerald E. Jaffe - Pembroke Pines FL
Steven Bott - Pembroke Pines FL
James H. Carter - Plantation FL

Assignee:

Coulter Corporation - Miami FL

International Classification:

C12Q 134
C12Q 102

US Classification:

435 18

Abstract:

An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.

US Patent:

56107129, Mar 11, 1997

Filed:

Oct 9, 1995

Appl. No.:

8/545424

Inventors:

Brian D. Schmitz - Pompano Beach FL
Steven E. Bott - Pembroke Pines FL
William H. Hart - Miami FL

Assignee:

Coulter Corporation - Miami FL

International Classification:

G01N 1502
G01N 2147

US Classification:

356335

Abstract:

A fiber optic spatial filter assembly for laser diffraction particle sizing apparatus utilizing a laser to generate a monochromatic light beam which is coupled to an optical fiber operating substantially in a monomode and creating a beam of light having a high degree of spatial coherence which is then passed through collimating lenses to interrogate and impinge upon the particles of matter through which the laser diffracted light passes. The light scattered by the particles is focused onto a Fourier plane and thereafter impinges upon a photooptical detector array, positioned coincident with the Fourier plane, for measuring the light intensities of the scattered light by scattering angle, thus enabling the computation of particle size.

US Patent:

56984111, Dec 16, 1997

Filed:

May 18, 1995

Appl. No.:

8/444051

Inventors:

Frank J. Lucas - Baca Raton FL
Gerald E. Jaffe - Pembroke Pines FL
Steven E. Bott - Pembroke Pines FL
James H. Carter - Plantation FL

Assignee:

Coulter Corporation - Miami FL

International Classification:

C12Q 102
C12Q 100

US Classification:

435 29

Abstract:

A method for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound is also disclosed.