Steven Cramer

US Patent:

6929747, Aug 16, 2005

Filed:

Feb 28, 2003

Appl. No.:

10/377080

Inventors:

Steven M. Cramer - Niskayuna NY, US
James A. Moore - East Greenbush NY, US
Sun Kyu Park - Daejon, KR
Nihal Tugcu - Woodbridge NJ, US

Assignee:

Renesselaer Polytechnic Institute - Troy NY

International Classification:

B01D015/08

US Classification:

210635, 210656, 210659, 2101982, 530413, 530416, 530417

Abstract:

A method for separating one or more components of a biomolecule mixture by means of an ion exchange chromatographic system operated in the displacement mode includes sequentially perfusing the system with a first solution including the biomolecule mixture, and a second solution including a displacer having a structure selected from formula I and formula II:.

US Patent:

7189324, Mar 13, 2007

Filed:

Jun 8, 2005

Appl. No.:

11/148887

Inventors:

Steven M. Cramer - Niskayuna NY, US
James A. Moore - East Greenbush NY, US
Sun Kyu Park - Daejon, KR
Nihal Tugcu - Franklin Park NJ, US

Assignee:

Rensselaer Polytechnic Institute - Troy NY

International Classification:

B01D 15/08

US Classification:

210635, 210656, 210659, 2101982, 530413, 530416, 530417

Abstract:

A method for separating one or more components of a biomolecule mixture by means of an ion exchange chromatographic system operated in the displacement mode includes sequentially perfusing the system with a first solution including the biomolecule mixture, and a second solution including a displacer having a structure selected from formula I and formula II:.

US Patent:

7439343, Oct 21, 2008

Filed:

Sep 1, 2005

Appl. No.:

11/217193

Inventors:

Kaushal Rege - Troy NY, US
Shanghui Hu - San Diego CA, US
Jonathan S. Dordick - Schenectady NY, US
Steven M. Cramer - Niskayuna NY, US

Assignee:

Rensselaer Polytechnic Institute - Troy NY

International Classification:

C07H 1/06
C07H 15/20
C07H 17/00

US Classification:

536 174, 536 136, 536 137, 536 138, 536 179, 536127

Abstract:

Aminoglycoside-polyamines are disclosed along with methods of use thereof in displacement chromatography and as DNA-binding ligands. The aminoglycoside-polyamines are derivatives of carbohydrates, such as sugars, amino sugars, deoxysugars, glycosides, nucleosides and their substituted counterparts. The subject polyamines possess a.

US Patent:

20050176937, Aug 11, 2005

Filed:

Apr 11, 2005

Appl. No.:

11/103293

Inventors:

Steven Cramer - Schenectady NY, US
Kaushal Rege - Troy NY, US
Jonathan Dordick - Schenectady NY, US
Nihal Tugcu - Franklin Park NJ, US

Assignee:

Rensselaer Polytechnic Institute - Troy NY

International Classification:

C07H021/04
C07K001/10

US Classification:

530416000, 536025400

Abstract:

A bioproduct may be selectively separated from one or more impurities by means of a displacement chromatography system that includes a solvent, a chromatographic resin and a chemically selective displacer. The method includes: dissolving the bioproduct and the one or more impurities in a solvent; loading the bioproduct and the one or more impurities, in the solvent, on a chromatographic resin; displacing the bioproduct from the chromatographic resin with chemically selective displacer; and retaining the one or more impurities on the chromatographic resin. For this method, the bioproduct and the impurities have similar binding affinity for the chromatographic resin in the absence of the displacer.

US Patent:

56060335, Feb 25, 1997

Filed:

Oct 19, 1995

Appl. No.:

8/545457

Inventors:

Steven M. Cramer - Schenectady NY
James A. Moore - Troy NY
Amitava Kundu - Troy NY
Yufei Li - Beijing, CN
Guhan Jayaraman - Madras, IN

International Classification:

C07K 114
C07K 116
C07K 118

US Classification:

530416

Abstract:

A method for the purification of proteins by displacement chromatography on ion exchange media using low molecular weight displacers is disclosed. Several classes of low molecular weight anionic species are exemplified, including aromatic rings having sulfonic acid or carboxylic acid moieties attached thereon, sulfated sugar derivatives, anionic antibiotics, and dendrimeric polymers. Novel compounds useful as displacers are dendrimers of formula ##STR1## wherein R. sup. 1 is lower alkyl, n is 2 to 6 and similar dendritic polymers based thereon.

US Patent:

54789249, Dec 26, 1995

Filed:

Feb 16, 1994

Appl. No.:

8/197146

Inventors:

Steven M. Cramer - Schenectady NY
James A. Moore - Troy NY
Amitava Kundu - Troy NY
Yufei Li - Beijing, CN
Guhan Jayaraman - Madras, IN

International Classification:

C07K 114
C07K 116
C07K 118
C07C21162

US Classification:

530416

Abstract:

A method for the purification of proteins by displacement chromatography on ion exchange media using low molecular weight displacers is disclosed. Several classes of low molecular weight, charged species are exemplified, including aminoacids, peptides, antibiotics and dendrimeric polymers. Novel compounds useful as displacers are dendrimers of formula ##STR1## wherein R. sup. 1 is lower alkyl, n is 2 to 6, and X is a common counter anion and similar dendritic polymers based thereon.

US Patent:

6239262, May 29, 2001

Filed:

Dec 30, 1998

Appl. No.:

9/223093

Inventors:

Steven M. Cramer - Schenectady NY
Abhinav A. Shukla - Bothell WA
Khurram M. Sunasara - Troy NY

Assignee:

Rensselaer Polytechnic Institute - Troy NY

International Classification:

C07K 114
B01D 1508

US Classification:

530417

Abstract:

A method for purification of proteins by displacement chromatography in hydrophobic interaction and reversed phase chromatographic systems uses low molecular weight (less than about 10,000) surface-active compounds as displacers. Examples of effective displacers are benzethonium chloride, benzyltributylammonium chloride, and tetrahexylammonium chloride.

US Patent:

20230053902, Feb 23, 2023

Filed:

Mar 9, 2022

Appl. No.:

17/690876

Inventors:

- Cambridge MA, US
- Troy NY, US
Laura Crowell - Cambridge MA, US
Alan Stockdale - Providence RI, US
Richard Dean Braatz - Arlington MA, US
Amos Enshen Lu - Cambridge MA, US
Steven Cramer - Troy NY, US
Steven Timmick - Troy NY, US
Nicholas Vecchiarello - Troy NY, US
Chaz Goodwine - Troy NY, US
Craig A. Mascarenhas - Brookline MA, US

Assignee:

Massachusetts Institute of Technology - Cambridge MA
Rensselaer Polytechnic Institute - Troy NY

International Classification:

C12M 1/00
C12M 1/34
C07K 1/20
C07K 16/00
G16C 20/20

Abstract:

Aspects of the present disclosure relate to systems and methods for manufacturing biologically-produced pharmaceutical products. Some of the systems described herein comprise an upstream component comprising a bioreactor and at least one filter (e.g., a filter probe) integrated with a downstream component comprising a purification module comprising at least a first partitioning unit and a second partitioning unit. In some embodiments; these integrated biomanufacturing systems may be operated under continuous or conditions and may be capable of efficiently producing pure, high-quality pharmaceutical products.