Steven G Robles

US Patent:

6834122, Dec 21, 2004

Filed:

Jan 22, 2001

Appl. No.:

09/767595

Inventors:

Mary M. Yang - San Diego CA
Edward J. Bylina - San Diego CA
William J. Coleman - San Diego CA
Michael R. Dilworth - Santa Cruz CA
Steven J. Robles - San Diego CA
Douglas C. Youvan - San Diego CA

Assignee:

Kairos Scientific, Inc. - San Diego CA

International Classification:

G06K 968

US Classification:

382227, 382260, 356303, 707 7

Abstract:

Complex multidimensional datasets generated by digital imaging spectroscopy can be organized and analyzed by applying software and computer-based methods comprising sorting algorithms. Combinations of these algorithms to images and graphical data, allow pixels or features to be rapidly and efficiently classified into meaningful groups according to defined criteria. Multiple rounds of pixel or feature selection may be performed based on independent sorting criteria. In one embodiment sorting by spectral criteria (e. g. , intensity at a given wavelength) is combined with sorting by temporal criteria (e. g. , absorbance at a given time) to identify microcolonies of recombinant organisms harboring mutated genes encoding enzymes having desirable kinetic attributes and substrate specificity. Restriction of the set of pixels analyzed in a subsequent sort based on criteria applied in an earlier sort (âsort and lockâ analyses) minimize computational and storage resources. User-defined criteria can also be incorporated into the sorting process by means of a graphical user interface that comprises a visualization tools including a contour plot, a sorting bar and a grouping bar, an image window, and a plot window that allow run-time interactive identification of pixels or features meeting one or more criteria, and display of their associated spectral or kinetic data.

US Patent:

7379601, May 27, 2008

Filed:

May 17, 2007

Appl. No.:

11/750107

Inventors:

Mary Mei-Iing Yang - San Diego CA, US
Edward J. Bylina - San Diego CA, US
William Joseph Coleman - San Diego CA, US
Michael Robert Dilworth - Santa Cruz CA, US
Steven J. Robles - San Jose CA, US
Douglas Charles Youvan - Frontenac KS, US

Assignee:

Kairos-Scientific Inc. - San Diego CA

International Classification:

G06K 9/62

US Classification:

382224, 382128, 128922, 356 2, 345 35, 345440, 702 19

Abstract:

Complex multidimensional datasets generated by digital imaging spectroscopy can be organized and analyzed by applying software and computer-based methods comprising sorting algorithms. Combinations of these algorithms to images and graphical data, allow pixels or features to be rapidly and efficiently classified into meaningful groups according to defined criteria. Multiple rounds of pixel or feature selection may be performed based on independent sorting criteria. In one embodiment sorting by spectral criteria (e. g. , intensity at a given wavelength) is combined with sorting by temporal criteria (e. g. , absorbance at a given time) to identify microcolonies of recombinant organisms harboring mutated genes encoding enzymes having desirable kinetic attributes and substrate specificity. Restriction of the set of pixels analyzed in a subsequent sort based on criteria applied in an earlier sort (“sort and lock” analyses) minimize computational and storage resources. User-defined criteria can also be incorporated into the sorting process by means of a graphical user interface that comprises a visualization tools including a contour plot, a sorting bar and a grouping bar, an image window, and a plot window that allow run-time interactive identification of pixels or features meeting one or more criteria, and display of their associated spectral or kinetic data.

US Patent:

20050114801, May 26, 2005

Filed:

Oct 1, 2004

Appl. No.:

10/956878

Inventors:

Mary Yang - San Diego CA, US
Edward Bylina - San Diego CA, US
William Coleman - San Diego CA, US
Michael Dilworth - Santa Cruz CA, US
Steven Robles - San Diego CA, US
Douglas Youvan - San Diego CA, US

International Classification:

G06K009/20

US Classification:

715961000

Abstract:

Complex multidimensional datasets generated by digital imaging spectroscopy can be organized and analyzed by applying software and computer-based methods comprising sorting algorithms. Combinations of these algorithms to images and graphical data, allow pixels or features to be rapidly and efficiently classified into meaningful groups according to defined criteria. Multiple rounds of pixel or feature selection may be performed based on independent sorting criteria. In one embodiment sorting by spectral criteria (e.g., intensity at a given wavelength) is combined with sorting by temporal criteria (e.g., absorbance at a given time) to identify microcolonies of recombinant organisms harboring mutated genes encoding enzymes having desirable kinetic attributes and substrate specificity. Restriction of the set of pixels analyzed in a subsequent sort based on criteria applied in an earlier sort (“sort and lock” analyses) minimize computational and storage resources. User-defined criteria can also be incorporated into the sorting process by means of a graphical user interface that comprises a visualization tools including a contour plot, a sorting bar and a grouping bar, an image window, and a plot window that allow run-time interactive identification of pixels or features meeting one or more criteria, and display of their associated spectral or kinetic data. These methods are useful for extracting information from imaging data in applications ranging from biology and medicine to remote sensing.

US Patent:

6738502, May 18, 2004

Filed:

Jun 2, 2000

Appl. No.:

09/585951

Inventors:

William Coleman - Mountain View CA
Michael A. Tanner - Hayward CA
Christopher M. Silva - Sunnyvale CA
Edward Bylina - San Jose CA
Steven J. Robles - San Jose CA
Michael R. Dilworth - Santa Cruz CA
Douglas C. Youvan - San Jose CA
Mary M. Yang - San Jose CA

Assignee:

Kairos Scientific, Inc. - San Diego CA

International Classification:

G06K 900

US Classification:

382133, 382167, 382191, 382287

Abstract:

The present invention provides an instrument and methods for a multispectral optical technique that can simultaneously classify individual biological cells within mixed populations. This invention, known as Multispectral Taxonomic Identification (MTID), shows that microscopy can be combined with a software analysis program to measure and categorize the fluorescence and other spectroscopically identifiable signals from complex populations of cells in situ, without cultivation. The invention thus enables high-throughput screening of cells for taxonomic classification.