ELLIOTT P. DAWSON - MURFREESBORO TN, US STEVEN J. SIMMONS - UNIONVILLE TN, US LORI J. RAY-COX - MURFREESBORO TN, US JENNIFER M. BAKER - NASHVILLE TN, US KRISTIE E. WOMBLE - FRANKLIN TN, US JUDITH MADDEN - VALENCIA CA, US SUBRAMANI SELLAPPAN - AURORA IL, US ANDREW HEARN - CHICAGO IL, US SAL SEMINARA - CHICAGO IL, US
Assignee:
BLOVENTURES, INC. - MURFREESBORO TN Celsis International plc - Chicago IL
International Classification:
C12Q 1/68
US Classification:
435 6
Abstract:
The invention relates to methods for the determination and detection of nucleic acids sequences in a sample. The nucleic acid may be RNA or DNA or both. The invention also relates to methods for the determination of the presence and species of various microorganisms in a sample. We have also identified a set of oligonucleotide nucleic acid sequences within the rRNAs of Gram-negative organisms that facilitates both the broad identification of Gram-negative organisms as a class when used as a pool, or in combination, for example in a hybridization assay. This set of oligonucleotides may detect sequences that are indicative of the presence of organisms of the broad class of Gram-negative organisms while exhibiting little or no false identification of Gram-positive organisms, and fungi, or other microorganisms. The assay includes concurrent incubation with at least one nucleotide sequence of interest, at least one nucleic acid probe, a fluorosurfactant, and a nuclease. The assay may further be employed to detect the presence of bacteria, fungi, or other microorganisms by use of additional specific probes, or to detect and/or identify target nucleic acid sequences in a sample. Further, the invention also relates to methods of reducing non-specific binding and facilitating complex formation in a binding assay. The binding assay may be, but is not limited to, a nucleic acid hybridization assay or an immunoassay. The invention also relates to methods of detection that employ at least one target of interest, which may be a nucleotide sequence, at least one probe, which may be a nucleic acid probe and a nuclease.
Monitoring And Analysis Of Nucleic Acid Hybridization State And Amplification Using L-Dna
- Nashville TN, US - Murfreesboro TN, US Steven J. Simmons - Unionville TN, US Elliott P. Dawson - Murfreesboro TN, US
Assignee:
Vanderbilt University - Nashville TN BioVentures, Inc. - Murfreesboro TN
International Classification:
C12Q 1/6818 C12Q 1/686 B01L 7/00
Abstract:
Systems, methods, and compositions for monitoring and analyzing nucleic acid hybridization state using L-DNA probes are described. The methods include adding L-DNA probes that can be fluorescently detected to a system including D-DNA. The L-DNA probes include primer, target, and antisense nucleotide sequences, and fluorescent dye compounds. The L-DNA probes are particularly useful for monitoring and analyzing various parameters during DNA amplification using the polymerase chain reaction.
License Records
Steven M. Simmons
License #:
CPT.002112 - Active
Issued Date:
Mar 24, 1998
Expiration Date:
Jun 30, 2017
Type:
Certified Pharmacy Technician
Steven Michael Simmons
License #:
8852 - Expired
Category:
Pharmacy
Issued Date:
Jul 1, 1977
Effective Date:
Jan 1, 1990
Type:
Pharmacist
Steven Trotter Simmons
License #:
MT015688T - Expired
Category:
Medicine
Type:
Graduate Medical Trainee
Name / Title
Company / Classification
Phones & Addresses
Steven Simmons
Select Service, LLC Carpenters
211 W 9 1/2 Mile Rd, Pensacola, FL 32534 8502324933
Steven J. Simmons Director
Northside Apostolic Church Construction · Religious Organization
213 W 9 1 Half Mile Rd, Pensacola, FL 32534 213 W 91 2 Mile Rd, Pensacola, FL 32534 8504765918
Steven J. Simmons Manager
Simmons Improvements LLC
340 Lowell Ln, Pensacola, FL 32514
Steven Simmons
Select Service, LLC Carpenters
211 W 9 1/2 Mile Rd, Pensacola, FL 32534 8502324933
Steven Simmons Principal
Club Z In Home Tutoring School/Educational Services
6410 Hwy 90, Milton, FL 32570
Steven Simmons Officer
BOVIS CONSTRUCTION CORP
Steven Simmons President
Florida State Marine, Inc Business Services at Non-Commercial Site · Nonclassifiable Establishments
5812 Quietwood Dr, Milton, FL 32583
Steven J. Simmons President, Vice President
Simco Builders Home Improvements - Additions
211 W 9 1/2 Mile Rd, Pensacola, FL 32534 209 E Johnson Ave, Pensacola, FL 32514 211 W 9 1 Half Mile Rd, Pensacola, FL 32534 8502324933
Glaucoma Consultants CapitalGlaucoma Consultants Of Capital Region 1240 New Scotland Rd STE 201, Slingerlands, NY 12159 5184757300 (phone), 5184759174 (fax)
Education:
Medical School University of Rochester School of Medicine and Dentistry Graduated: 1981
Procedures:
Retinal Detachment Repair Lens and Cataract Procedures Ophthalmological Exam
Dr. Simmons graduated from the University of Rochester School of Medicine and Dentistry in 1981. He works in Slingerlands, NY and specializes in Ophthalmology. Dr. Simmons is affiliated with Albany Medical Center and Albany Memorial Hospital.
Dr. Simmons graduated from the University of Tennessee College of Medicine at Memphis in 1993. He works in Vienna, VA and specializes in Internal Medicine.
charged in January in an alleged reselling scheme involving tickets to the hit Broadway musical "Hamilton." Since then, prosecutors have expanded the case and now claim that he and another man, Steven Simmons, used a hedge fund, Sentinel Growth Fund Management, to scam millions of dollars from investors.
Date: Sep 06, 2017
Category: Top Stories
Source: Google
Two Men Allegedly Ran An $81 Million Ponzi Scheme Around 'Hamilton' Tickets
Did we say a little bit? What we meant to say was an $81 million-dollar Ponzi scheme built on tickets that didnt exist. According to Reuters, Joseph Meli and Steven Simmons were arrested on January 27 and charged with securities fraud, conspiracy and wire fraud. The two men are accused of running
U.S. attorneys office in the Southern District of New York, Meli and Steven Simmons, 48, of Wilton, Conn., were arrested Friday on charges of conspiracy, securities fraud and wire fraud for participating in an alleged Ponzi scheme that bilked investors to repay earlier investors in a hedge fund.
Date: Jan 28, 2017
Source: Google
Two men charged in alleged Ponzi scheme for 'Hamilton' tickets
S. attorneys office in the Southern District of New York, Meli and Steven Simmons, 48, of Wilton, Conn., were arrested Friday on charges of conspiracy, securities fraud and wire fraud for participatingin an alleged Ponzi scheme that bilked investors to repay earlier investors in a hedge fund.