Steven T Simmons

US Patent:

20090203017, Aug 13, 2009

Filed:

Jan 23, 2009

Appl. No.:

12/359263

Inventors:

ELLIOTT P. DAWSON - MURFREESBORO TN, US
STEVEN J. SIMMONS - UNIONVILLE TN, US
LORI J. RAY-COX - MURFREESBORO TN, US
JENNIFER M. BAKER - NASHVILLE TN, US
KRISTIE E. WOMBLE - FRANKLIN TN, US
JUDITH MADDEN - VALENCIA CA, US
SUBRAMANI SELLAPPAN - AURORA IL, US
ANDREW HEARN - CHICAGO IL, US
SAL SEMINARA - CHICAGO IL, US

Assignee:

BLOVENTURES, INC. - MURFREESBORO TN
Celsis International plc - Chicago IL

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

The invention relates to methods for the determination and detection of nucleic acids sequences in a sample. The nucleic acid may be RNA or DNA or both. The invention also relates to methods for the determination of the presence and species of various microorganisms in a sample. We have also identified a set of oligonucleotide nucleic acid sequences within the rRNAs of Gram-negative organisms that facilitates both the broad identification of Gram-negative organisms as a class when used as a pool, or in combination, for example in a hybridization assay. This set of oligonucleotides may detect sequences that are indicative of the presence of organisms of the broad class of Gram-negative organisms while exhibiting little or no false identification of Gram-positive organisms, and fungi, or other microorganisms. The assay includes concurrent incubation with at least one nucleotide sequence of interest, at least one nucleic acid probe, a fluorosurfactant, and a nuclease. The assay may further be employed to detect the presence of bacteria, fungi, or other microorganisms by use of additional specific probes, or to detect and/or identify target nucleic acid sequences in a sample. Further, the invention also relates to methods of reducing non-specific binding and facilitating complex formation in a binding assay. The binding assay may be, but is not limited to, a nucleic acid hybridization assay or an immunoassay. The invention also relates to methods of detection that employ at least one target of interest, which may be a nucleotide sequence, at least one probe, which may be a nucleic acid probe and a nuclease.

US Patent:

20180171393, Jun 21, 2018

Filed:

May 2, 2016

Appl. No.:

15/571153

Inventors:

- Nashville TN, US
- Murfreesboro TN, US
Steven J. Simmons - Unionville TN, US
Elliott P. Dawson - Murfreesboro TN, US

Assignee:

Vanderbilt University - Nashville TN
BioVentures, Inc. - Murfreesboro TN

International Classification:

C12Q 1/6818
C12Q 1/686
B01L 7/00

Abstract:

Systems, methods, and compositions for monitoring and analyzing nucleic acid hybridization state using L-DNA probes are described. The methods include adding L-DNA probes that can be fluorescently detected to a system including D-DNA. The L-DNA probes include primer, target, and antisense nucleotide sequences, and fluorescent dye compounds. The L-DNA probes are particularly useful for monitoring and analyzing various parameters during DNA amplification using the polymerase chain reaction.