Susan B Deak

US Patent:

53407237, Aug 23, 1994

Filed:

Apr 16, 1992

Appl. No.:

7/869745

Inventors:

Charles D. Boyd - Princeton NJ
Susan B. Deak - Somerset NJ

Assignee:

University of Medicine & Dentistry of New Jersey - Piscataway NJ

International Classification:

C12Q 102
C12Q 100
G01N 3353
C12N 500

US Classification:

435 29

Abstract:

The present invention pertains to a method for diagnosing for an aneurysm in a patient which comprises the steps of (a) explanting a skin section containing dermal fibroblast from the patient; (b) culturing the fibroblast to confluence in a culture medium; (c) incubating the cultured fibroblast with labeled proline to provide labeled procollagen in the culture medium; (d) separating the culture medium from the labeled procollagen and treating the labeled procollagen with a solution of protease inhibitor; (e) separating and purifying the labeled procollagen from the solution of protease inhibitor; (f) subjecting the labeled procollagen to protease digestion specific for non-collagenous proteins to form a collagenous mixture; (g) analyzing the collagenous mixture for the ratio of type I collagen to type III collagen; (h) analyzing a control collagenous mixture for the ratio of type I collagen to type III collagen; and (i) comparing the ratio of type I collagen to type III collagen in the collagenous mixture of step (g) to the control mixture of step (h), respectively.