Susan H Little

US Patent:

20190025305, Jan 24, 2019

Filed:

Dec 11, 2017

Appl. No.:

15/838046

Inventors:

- South San Francisco CA, US
Mary T. Wrin - Fremont CA, US
Susan Little - San Diego CA, US
Christos J. Petropoulos - Half Moon Bay CA, US
Neil T. Parkin - Belmont CA, US
Jeannette Whitcomb - San Mateo CA, US
Wei Huang - Foster City CA, US

Assignee:

Monogram Biosciences, Inc. - South San Francisco CA

International Classification:

G01N 33/569
C12Q 1/6897
G01N 33/50
C12Q 1/70
C07K 14/005
C07K 16/10
C07K 14/16
C07K 16/08

Abstract:

The present invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell.

US Patent:

20160209411, Jul 21, 2016

Filed:

Oct 29, 2015

Appl. No.:

14/926837

Inventors:

- South San Francisco CA, US
Mary T. Wrin - Fremont CA, US
Susan Little - San Diego CA, US
Christos J. Petropoulos - Half Moon Bay CA, US
Neil T. Parkin - Belmont CA, US
Jeannette Whitcomb - San Mateo CA, US
Wei Huang - Foster City CA, US

International Classification:

G01N 33/569

Abstract:

Provided are methods for identifying whether a compound inhibits entry of a virus into a cell. The method may include obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus and co-transfecting it into a first cell along with a viral expression vector which lacks a nucleic acid encoding the envelope protein. The method may further include contacting the viral particles produced by the first cell with a second cell to which the virus binds in the absence and presence of the compound and measuring the amount of signal produced by the second cell.