Susan C Stone

US Patent:

20060128009, Jun 15, 2006

Filed:

Jan 17, 2006

Appl. No.:

11/333483

Inventors:

Anthony Cerrone - New Haven CT, US
Edward Lakatos - Bethel CT, US
Michael Gambini - Bolton MA, US
Eugene Young - Foster City CA, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US

International Classification:

C12M 1/34

US Classification:

435288700, 435288400, 435303100

Abstract:

An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

US Patent:

20060199259, Sep 7, 2006

Filed:

Apr 18, 2006

Appl. No.:

11/406148

Inventors:

Anthony Cerrone - Northford CT, US
Edward Lakatos - Bethel CT, US
Michael Gambini - Monroe CT, US
Eugene Young - Foster City CA, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US
John Atwood - Redding CT, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12M 1/34

US Classification:

435288700, 435287200

Abstract:

An instrument for monitoring replication of DNA is provided.

US Patent:

20070148761, Jun 28, 2007

Filed:

Feb 13, 2007

Appl. No.:

11/705856

Inventors:

Anthony Cerrone - New Haven CT, US
Edward Lakatos - Bethel CT, US
Michael Gambini - Monroe CT, US
Eugene Young - Foster City CA, US
Judith Atwood - Redding CT, US
John Atwood - Redding CT, US
Susan Stone - Charlotte VT, US

International Classification:

C12M 1/34

US Classification:

435287200, 435288700

Abstract:

An optical instrument monitors PCR replication of DNA in a reaction apparatus having vials of reaction ingredients. A beam splitter passes an excitation beam to the vials. An emission beam from the reaction ingredients can be passed by the beam splitter to a detector from which a processor can compute DNA concentration. A reference strip with a plurality of reference emitters can be provided which emits reference beams of different intensity, from which the processor can select an optimum emitter for compensating for drift. Exposure time can be automatically adjusted for keeping within optimum dynamic ranges of the detector and processor. A module of the beam splitter and associated optical filters can be associated with a selected dye, and can be replaceable for different dyes.

US Patent:

20070154939, Jul 5, 2007

Filed:

Feb 26, 2007

Appl. No.:

11/711416

Inventors:

Anthony Cerrone - New Haven CT, US
Edward Lakatos - Bethel CT, US
Michael Gambini - Monroe CT, US
Eugene Young - Foster City CA, US
John Atwood - Redding CT, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 1/68
G06F 19/00
C12M 3/00

US Classification:

435006000, 435287200, 702020000

Abstract:

An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

US Patent:

20070238161, Oct 11, 2007

Filed:

May 17, 2007

Appl. No.:

11/804151

Inventors:

Anthony Cerrone - New Haven CT, US
Edward Lakatos - Bethel CT, US
Michael Gambini - Monroe CT, US
Eugene Young - Foster City CA, US
Susan Stone - Charlotte VT, US
Judith Atwood - Redding CT, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12M 1/34
C12M 1/36

US Classification:

435286100, 435287200

Abstract:

An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.