Suzanne M Chan

US Patent:

20060084792, Apr 20, 2006

Filed:

Aug 1, 2005

Appl. No.:

11/194991

Inventors:

Chad Paavola - Mountain View CA, US
Jonathan Trent - Watsonville CA, US
Suzanne Chan - Oakland CA, US
Yi-Fen Li - Sunnyvale CA, US
R. McMillan - San Francisco CA, US
Hiromi Kagawa - Sunnyvale CA, US

International Classification:

C07K 14/195
C07H 21/04
C12P 21/06
C12N 15/74
C12N 1/21

US Classification:

530350000, 435069100, 435252300, 435320100, 536023700

Abstract:

The present invention provides chaperonin polypeptides which are modified to include N-terminal and C-terminal ends that are relocated from the central pore region to various different positions in the polypeptide which are located on the exterior of the folded modified chaperonin polypeptide. In the modified chaperonin polypeptide, the naturally-occurring N-terminal and C-terminal ends are joined together directly or with an intervening linker peptide sequence. The relocated N-terminal or C-terminal ends can be covalently joined to, or bound with another molecule such as a nucleic acid molecule, a lipid, a carbohydrate, a second polypeptide, or a nanoparticle. The modified chaperonin polypeptides can assemble into double-ringed chaperonin structures. Further, the chaperonin structures can organize into higher order structures such as nanofilaments or nanoarrays which can be used to produce nanodevices and nanocoatings.