Sylvia Z Schade from 189 Delaplaine Rd, North Riverside, IL 60546, age 91, Phone: 7084479226

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Location:

Chicago, IL

Industry:

Religious Institutions

Fluorescence Polarization Instruments And Methods For Detection Of Exposure To Biological Materials By Fluorescence Polarization Immunoassay Of Saliva, Oral Or Bodily Fluids
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US Patent:

7408640, Aug 5, 2008
Filed:

Nov 5, 2003
Appl. No.:

10/700868
Inventors:

Malford E. Cullum - Grayslake IL, US
Lloyd G. Simonson - Spring Grove IL, US
Sylvia Z. Schade - Riverside IL, US
Linda A. Lininger - Grayslake IL, US
Alan L. McArthur - Mokena IL, US
Assignee:

The United States of America as represented by the Secretary of the Navy - Washington DC
International Classification:

G01J 3/00
G01J 4/00
C12M 1/00
C12M 3/00
US Classification:

356368, 356300, 356364, 4352831, 4352871, 4352872
Abstract:

The inventive subject matter relates to a method for detecting the presence of a biological substance of interest in a test sample of saliva or oral fluid, comprising combining said test sample with a fluorescence-labeled ligand to said biological substance and detecting a change in the fluorescence polarization of said test sample produced by binding of said fluorescence-labeled ligand to said biological substance. In one aspect of the inventive subject matter, said method comprises additional steps for comparing the fluorescence polarization of said test sample with the fluorescence polarization of a control solution. Also provided is a miniaturized, portable apparatus for measuring the fluorescence polarization of a liquid sample.

Fluorescence Polarization Instruments And Methods For Detection Of Exposure To Biological Materials By Fluorescence Polarization Immunoassay Of Saliva, Oral Or Bodily Fluids
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US Patent:

8114582, Feb 14, 2012
Filed:

Jun 27, 2008
Appl. No.:

12/163412
Inventors:

Malford E. Cullum - Grayslake IL, US
Lloyd G. Simonson - Spring Grove IL, US
Sylvia Z. Schade - Riverside IL, US
Linda A. Lininger - Grayslake IL, US
Alan L. McArthur - Mokena IL, US
Assignee:

The United States of America as represented by the Secretary of the Navy - Washington DC
International Classification:

C12Q 1/00
G01N 33/53
G01N 33/554
G01N 3/00
A61K 39/00
A61K 39/07
A61K 39/04
US Classification:

435 4, 435 71, 435 732, 435 792, 4241841, 4242461, 4242481
Abstract:

The inventive subject matter relates to a method for detecting the presence of a biological substance of interest in a test sample of saliva or oral fluid, comprising combining said test sample with a fluorescence-labeled ligand to said biological substance and detecting a change in the fluorescence polarization of said test sample produced by binding of said fluorescence-labeled ligand to said biological substance. In one aspect of the inventive subject matter, said method comprises additional steps for comparing the fluorescence polarization of said test sample with the fluorescence polarization of a control solution. Also provided is a miniaturized, portable apparatus for measuring the fluorescence polarization of a liquid sample.

Fluorescence Polarization Assays Of Enzymes And Substrates Therefore
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US Patent:

58043950, Sep 8, 1998
Filed:

Dec 1, 1995
Appl. No.:

8/566390
Inventors:

Sylvia Zottu Schade - Riverside IL
Michael Ernest Jolley - Round Lake IL
Assignee:

The United States of America as represented by the Secretary of the Navy - Washington DC
International Classification:

G01N 33573
G01N 3353
C12Q 100
US Classification:

435 74
Abstract:

Fluorescent-labeled substrates are provided for fluorescence polarization says of enzymes. These substrates are proteins labeled with derivatives of BODIPY. RTM. , 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene. The BODIPY. RTM. fluorescent tag of the present invention is pH independent, and can be used over a pH range of from about 2 to about 11. Thus one can assay, in real time, enzymes with pH maxima at pH below 7 using fluorescence polarization methodology, which could not be done with fluorescein derivatives. Different enzymes can be compared using the same BODIPY. RTM. conjugate by merely changing the buffer system which changes the pH conditions. Fluorescence polarization assays of enzyme activity can be performed in the presence of whole bacteria and other finely suspended particles, such as might be present in tissue homogenates or cellular material. This is particularly useful for chairside assays on dental plaque or clinical assays on bacteria or tissue or exudates.

Rapid Microbial Protease Assay
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US Patent:

57416591, Apr 21, 1998
Filed:

Jan 4, 1996
Appl. No.:

8/583170
Inventors:

Stephen Alden Ralls - Great Lakes IL
Lloyd Grant Simonson - Deerfield IL
Sylvia Zottu Schade - Riverside IL
Assignee:

The United States of America as represented by the Secretary of the Navy - Washington DC
International Classification:

C12Q 137
C12Q 104
C12Q 152
A01N 3718
US Classification:

435 23
Abstract:

An assay for detecting microbial protease activity in clinical and laboray samples is described which comprises gathering a sample suspected of containing certain microorganisms having the desired protease activity; immobilizing the microorganisms in the sample on a solid phase substrate; contacting the immobilized microorganisms with an enzymatic substrate producing an enzymatic substrate end-product; contacting the enzymatic substrate end-product with a chemical enhancing reagent producing a detectable chromogenic reaction which varies in intensity with the level of protease activity in the sample; and detecting the chromogenic reaction whereby the semi-quantitative presence of the protease activity in the sample is determined. The device for conducting these assays is a frame or support which holds a solid phase substrate capable of binding the sought microorganisms of interest while permitting drainage of other materials or fluids, which may contain host proteases, away from the immobilized microorganisms.