Tam Dinh Vu

US Patent:

20230034263, Feb 2, 2023

Filed:

Nov 19, 2020

Appl. No.:

17/778302

Inventors:

- Oakland CA, US
Enrico GRATTON - San Clemente CA, US
Jan ZIMAK - Irvine CA, US
Tam VU - San Jose CA, US
Alexander VALLMITJANA - Irvine CA, US
Joshua GU - Irvine CA, US
Per Niklas HEDDE - Irvine CA, US
Lorenzo SCIPIONI - Irvine CA, US

International Classification:

C12Q 1/6841
C12Q 1/682
C12Q 1/6818

Abstract:

In alternative embodiments, provided are compositions, including products of manufacture and kits, and methods, for in situ spatial profiling of biological materials such as DNA, RNA and protein in cells, tissues, and organisms for investigating biology and for conducting biomarker/drug discovery and development, and for clinical pathology and diagnosis. In alternative embodiments, provided are compositions, including products of manufacture and kits, and methods, for spatially determining, visualizing or quantifying target biological materials comprising in situ staining of a biological sample with one or a plurality of probes that are labeled with light-emitting moieties that exhibit or are encoded with distinct luminescence lifetime (and, optionally, spectrum) characteristics; followed by time-resolved luminescence imaging, measurement and analysis.