Terri G Dunahay

US Patent:

56610173, Aug 26, 1997

Filed:

Mar 15, 1995

Appl. No.:

8/404732

Inventors:

Terri Goodman Dunahay - Boulder CO
Paul G. Roessler - Golden CO
Eric E. Jarvis - Boulder CO

International Classification:

A01H 1300
C12N 113
C12N 1529
C12N 1531
C12N 1579

US Classification:

4351723

Abstract:

Disclosed is a method to transform chlorophyll C-containing algae which includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further specifically disclosed are molecules pACCNPT10, pACCNPT4. 8 and pACCNPT5. 1.