Terry Moore Phillips

US Patent:

6969605, Nov 29, 2005

Filed:

Jul 16, 2001

Appl. No.:

09/906243

Inventors:

Charles W. Anderson - Woodbine MD, US
C. Brent Bargeron - Columbia MD, US
Richard C. Benson - Highland MD, US
Micah A. Carlson - Baltimore MD, US
Allan B. Fraser - Woodbine MD, US
John D. Groopman - Owings Mills MD, US
Harvey W. Ko - Ellicott City MD, US
David R. Kohler - Ellicott City MD, US
Terry E. Phillips - Ellicott City MD, US
Paul T. Strickland - Baltimore MD, US

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

C12M003/00

US Classification:

4352871, 2101982, 210656, 210659, 2504581, 2504591, 2504611, 2504621, 422 56, 422 69, 422 70, 4352872, 4352885, 4352887, 436161, 436172, 436518, 436529, 436530, 436531, 436534, 436536

Abstract:

A hand held, self-contained, automatic, low power and rapid sensor platform for detecting and quantifying a plurality of analytes. A sample solution potentially containing an unknown amount of an analyte is passed through an affinity column which contains antibodies to which the analyte binds thereby extracting the analyte. The affinity column is then rinsed to remove any other chemicals that may fluoresce. The rinsed affinity column is then eluted with a known volume of elution fluid causing the analyte to release from the antibody and dissolve in the fluid (eluant). The eluant is then placed in the quartz cuvette of a fluorometer. The analyte suspended in the eluant fluoresces at a waveband which is different than that of the light source that excites it. The amount of fluorescence is measured and the level of analyte determined. The rinsing and elution fluids, and/or the affinity column can be placed in a module for easy insertion and removal from the sensor when depleted or when the sensor is to be used against a different analyte.

US Patent:

62618482, Jul 17, 2001

Filed:

May 8, 1998

Appl. No.:

9/074644

Inventors:

Charles W. Anderson - Woodbine MD
C. Brent Bargeron - Columbia MD
Richard C. Benson - Highland MD
Micah A. Carlson - Baltimore MD
Allan B. Fraser - Woodbine MD
John D. Groopman - Owings Mills MD
Harvey W. Ko - Ellicott City MD
David R. Kohler - Ellicott City MD
Terry E. Phillips - Ellicott City MD
Paul T. Strickland - Baltimore MD

Assignee:

The Johns Hopkins University - Baltimore MD

International Classification:

G01N 33543

US Classification:

436518

Abstract:

A hand held, self-contained, automatic, low power and rapid sensor platform for detecting and quantifying a plurality of analytes. A sample solution potentially containing an unknown amount of an analyte is passed through an affinity column which contains antibodies to which the analyte binds thereby extracting the analyte. The affinity column is then rinsed to remove any other chemicals that may fluoresce. The rinsed affinity column is then eluted with a known volume of elution fluid causing the analyte to release from the antibody and dissolve in the fluid (eluant). The eluant is then placed in the quartz cuvette of a fluorometer. The analyte suspended in the eluant fluoresces at a waveband which is different than that of the light source that excites it. The amount of fluorescence is measured and the level of analyte determined. The rinsing and elution fluids, and/or the affinity column can be placed in a module for easy insertion and removal from the sensor when depleted or when the sensor is to be used against a different analyte.