Theodore A Davis

US Patent:

6958230, Oct 25, 2005

Filed:

Sep 22, 2003

Appl. No.:

10/668047

Inventors:

Keith D. Lunnen - Essex MA, US
Theodore Davis - Boxford MA, US
Geoffrey G. Wilson - S. Hamilton MA, US

Assignee:

New England Biolabs, Inc. - Beverly MA

International Classification:

C12N009/22
C12N015/55

US Classification:

435199, 4353201, 4352523, 536 232

Abstract:

The present invention relates to: recombinant DNA encoding the SbfI restriction endonuclease as well as the SbfI methylase, and expression of the SbfI restriction endonuclease and SbfI methylase in cells containing the recombinant DNA; and methods for cloning the SbfI restriction gene (sbfIR) from species Bf-61 into by PCR. The method relied on primers based on DNA sequences predicted from amino acid sequences of the purified SbfI restriction endonuclease.

US Patent:

7115407, Oct 3, 2006

Filed:

Jul 10, 2003

Appl. No.:

10/616624

Inventors:

Richard D. Morgan - Middletown MA, US
Tanya Bhatia - Peabody MA, US
Theodore Davis - Boxford MA, US
Lindsay Lovasco - Salem MA, US

Assignee:

New England BioLabs, Inc. - Ipswich MA

International Classification:

C12N 9/22
C12N 15/55

US Classification:

435199, 4353201, 4352523, 536 232

Abstract:

In accordance with the present invention, there is provided a DNA (deoxyribonucleic acid) fragment which encodes the MmeI type II restriction endonuclease enzyme. This one polypeptide possesses two related enzymatic functions; namely an endonuclease activity which recognizes the DNA sequence 5′-TCC(Pu)AC-3′ and cleaves as indicated by the arrows:.

US Patent:

7319014, Jan 15, 2008

Filed:

Feb 20, 2004

Appl. No.:

10/545905

Inventors:

Ellen Guthrie - Andover MA, US
Theodore Davis - Boxford MA, US
Jack Benner, II - South Hamilton MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12Q 1/42
C12P 21/06
C12N 9/16

US Classification:

435 21, 435 681, 435196

Abstract:

Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65 C. and efficiency of dephosphorylation activity at approximately neutral pH.

US Patent:

41366700, Jan 30, 1979

Filed:

Jun 13, 1977

Appl. No.:

5/805688

Inventors:

Theodore L. Davis - Medway MA

International Classification:

F24J 302

US Classification:

126271

Abstract:

A solar heating system including an energy absorbent collector dish for receiving solar heat, a supply tube for distributing a heat transfer fluid over the collector dish, a drain pipe for removing the heat transfer liquid from the collector dish and an optical director including an array of converging lenses oriented to receive radiant energy from the sun and to direct the energy onto the collector dish. The array of converging lenses comprises a matrix of double convex lenses that form one half of a hemispherical optical dome, the other half of which possesses an inner reflective surface for reflecting radiant energy onto the collector dish and the optical dome is oriented such that some portion of the lens array faces the sun during an entire day's movement.

US Patent:

D3425940, Dec 28, 1993

Filed:

Jun 8, 1992

Appl. No.:

7/895390

Inventors:

Theodore L. Davis - New York NY

US Classification:

D 2860

US Patent:

20210388433, Dec 16, 2021

Filed:

Aug 17, 2021

Appl. No.:

17/445286

Inventors:

- Ipswich MA, US
Zhiyi Sun - Gloucester MA, US
Shengxi Guan - Stoneham MA, US
Lana Saleh - Hamilton MA, US
Laurence Ettwiller - Beverly MA, US
Theodore B. Davis - Boxford MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12Q 1/6858
C12Q 1/6806
C12Q 1/6827
C12N 9/02
C12Q 1/6869
C12Q 1/6872

Abstract:

A method for identifying any of the presence, location and phasing of modified cytosines (C) in long stretches of nucleic acids is provided. In some embodiments, the method may comprise (a) reacting a first portion of a nucleic acid sample containing at least one C and/or at least one modified C with a DNA glucosyltransferase and a cytidine deaminase to produce a first product and/or reacting a second portion of the sample with a dioxygenase, optionally a DNA glucosyltransferase and a cytidine deaminase to produce a second product and; (b) comparing the sequences from the first and optionally the second product obtained in (a), or amplification products thereof, with each other and/or an untreated reference sequence to determine which Cs in the initial nucleic acid fragment are modified. A modified TET methylcytosine dioxygenase with improved efficiency compared to unmodified TET2 at converting methylcytosine to carboxymethylcytosine is also provided.

US Patent:

20210207200, Jul 8, 2021

Filed:

Feb 22, 2021

Appl. No.:

17/181351

Inventors:

- Ipswich MA, US
Theodore B. Davis - Boxford MA, US
Shengxi Guan - Stoneham MA, US
Zhiyi Sun - Gloucester MA, US
Laurence Ettwiller - Beverly MA, US
Lana Saleh - Hamilton MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12Q 1/6827
C12Q 1/6806
C12N 9/02
C12N 9/78

Abstract:

Methods and compositions are provided for identifying any of the presence, location and phasing of methylated and/or hydroxymethylated cytosines in nucleic acids including long stretches of DNA. In some embodiments, the method may comprise reacting a first portion (aliquot) of a nucleic acid sample with a dioxygenase and optionally a glucosyltransferase in a reaction mixture containing the nucleic acid followed by a reaction with a cytidine deaminase to detect and optionally map C in a DNA. Optionally, a second portion can be reacted with glucosyltransferase followed by reaction with a cytidine deaminase to detect and optionally map C in a DNA.

US Patent:

20190185919, Jun 20, 2019

Filed:

Feb 27, 2019

Appl. No.:

16/287604

Inventors:

- Ipswich MA, US
Theodore B. Davis - Boxford MA, US
Shengxi Guan - Ipswich MA, US
Zhiyi Sun - Gloucester MA, US
Laurence Ettwiller - Ipswich MA, US
Lana Saleh - Hamilton MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12Q 1/6827
C12N 9/78
C12N 9/02
C12Q 1/6806

Abstract:

Methods and compositions are provided for identifying any of the presence, location and phasing of methylated and/or hydroxymethylated cytosines in nucleic acids including long stretches of DNA. In some embodiments, the method may comprise reacting a first portion (aliquot) of a nucleic acid sample with a dioxygenase and optionally a glucosyltransferase in a reaction mixture containing the nucleic acid followed by a reaction with a cytidine deaminase to detect and optionally map C in a DNA. Optionally, a second portion can be reacted with glucosyltransferase followed by reaction with a cytidine deaminase to detect and optionally map C in a DNA

Address:

1221 Ave Of The Americas, New York, NY 10020

Phone:

5856153533

VIN:

5XYKT3A15CG188537

Make:

KIA

Model:

SORENTO

Year:

2012