Theodore E Straub

US Patent:

20230030218, Feb 2, 2023

Filed:

Oct 10, 2022

Appl. No.:

17/963135

Inventors:

- Carlsbad CA, US
Theodore E. STRAUB - Lexington MA, US
Evan W. FOSTER - San Mateo CA, US
Michael C. PALLAS - San Bruno CA, US

International Classification:

G01N 35/10
C40B 60/14
B01L 3/00
B01J 19/00

Abstract:

An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

US Patent:

20230022224, Jan 26, 2023

Filed:

Jul 11, 2022

Appl. No.:

17/861854

Inventors:

- Carlsbad CA, US
Nivedita Sumi MAJUMDAR - San Bruno CA, US
Jeffrey A. MARKS - Mountain View CA, US
Theodore E. STRAUB - Lexington MA, US
Ryan J. TALBOT - Stamford CT, US
Kevin S. BODNER - Belmont CA, US
David HOARD - Escondido CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

G06T 11/20
G16B 45/00

Abstract:

A method comprises displaying, on a graphical user interface (GUI), a first data visualization comprising a positional representation of a plurality of reaction sites from at least a portion of a substrate, wherein the positional representation of the first data visualization simultaneously indicates relative data quality of the plurality of reaction sites; and altering the first data visualization displayed on the GUI to a second data visualization, the second data visualization comprising the positional representation of the plurality of reaction sites from the at least the portion of the substrate, wherein the positional representation of the second data visualization simultaneously indicates relative data quality of the plurality of reaction sites from at least the portion of the substrate based on a comparison of the data quality values of the plurality of reaction sites an adjusted quality value threshold.

US Patent:

20210042972, Feb 11, 2021

Filed:

Jul 24, 2020

Appl. No.:

16/937634

Inventors:

- Carlsbad CA, US
Nivedita Sumi MAJUMDAR - San Bruno CA, US
Jeffrey A. MARKS - Mountain View CA, US
Theodore E. STRAUB - Lexington MA, US
Ryan J. TALBOT - Stamford CT, US
Kevin S. BODNER - Belmont CA, US
David HOARD - Escondido CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

G06T 11/20
G16B 45/00

Abstract:

A method for generating a data visualization is provided. The method includes displaying a representation of a portion of detected data from a substrate to a user. The method further includes generating a data quality value for the portion of detected data and displaying, along with the representation of the portion of detected data, an indication of data quality value for the portion of detected data. The method further includes selecting, by the user, a quality value threshold, and displaying an adjusted indication of data quality value for the portion of detected data meeting the quality value threshold.

US Patent:

20200319221, Oct 8, 2020

Filed:

Apr 20, 2020

Appl. No.:

16/852830

Inventors:

- Carlsbad CA, US
Theodore E. STRAUB - Lexington MA, US
Evan W. FOSTER - San Mateo CA, US
Michael C. PALLAS - San Bruno CA, US

International Classification:

G01N 35/10
C40B 60/14
B01L 3/00
B01J 19/00

Abstract:

An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

US Patent:

20190381502, Dec 19, 2019

Filed:

May 30, 2019

Appl. No.:

16/426631

Inventors:

- Carlsbad CA, US
James C. Nurse - Sequim WA, US
Gary Lim - San Francisco CA, US
Theodore E. Straub - Lexington MA, US
Eliodor Ghenciu - Atherton CA, US
Evan Foster - San Mateo CA, US
Jorge Fonseca - East Palo Alto CA, US
Kevin Maher - Woodside CA, US

International Classification:

B01L 3/00
C12Q 1/6806
G03F 7/20
C12Q 1/686

Abstract:

A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

US Patent:

20190201891, Jul 4, 2019

Filed:

Mar 4, 2019

Appl. No.:

16/291757

Inventors:

- Carlsbad CA, US
Evan FOSTER - San Mateo CA, US
Jorge FONSECA - East Palo Alto CA, US
James NURSE - Westport WA, US
Theodore STRAUB - Burlingame CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

B01L 3/00
B01L 7/00
B01L 9/00
C12Q 1/686

Abstract:

A system for processing a plurality of biological samples contains a support and a temperature controller. The suppose is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.

US Patent:

20170081712, Mar 23, 2017

Filed:

Sep 22, 2016

Appl. No.:

15/273200

Inventors:

- Carlsbad CA, US
Shoulian DONG - Mountain View CA, US
Theodore E. STRAUB - Lexington MA, US
David N. KEYS - Alameda CA, US

International Classification:

C12Q 1/68
G01N 27/30
G01N 27/414

Abstract:

A system for detecting one or more target molecules includes a reaction device and a temperature controller. The reaction device comprises a plurality of reaction sites and plurality of field effect transistors corresponding to the plurality of reaction sites. At least two of the reaction sites may receive a reaction solution including two target molecules. The temperature controller is configured to control a temperature of at least one of the reaction sites or the reaction solution received by the at least one reaction site. At least some of the reaction sites may receive a reagent supporting an amplification reaction. When the reaction is subjected to an amplification assay, an amplified product is produced in the at least some of the reaction sites. The plurality of field effect transistors are configured to detect at least one of the amplified products.

US Patent:

20170088879, Mar 30, 2017

Filed:

Sep 29, 2016

Appl. No.:

15/280160

Inventors:

- Carlsbad CA, US
Nivedita Sumi MAJUMDAR - San Bruno CA, US
Theodore E. STRAUB - Lexington MA, US

International Classification:

C12Q 1/68

Abstract:

Systems and methods are described for quantifying a target nucleic acid. A sample comprising a target nucleic acid is segregated into a first plurality of the reaction volumes containing at least one target nucleic acid molecule and a second plurality of the reaction volumes containing no target nucleic acid molecules. The reaction volumes are subjected to an amplification assay, wherein the amplification assay is configured to amplify the target nucleic acid. An indicator of amplification is detected or measured in at least some of the plurality of reaction volumes. The target nucleic acid is quantified based on the detection or measurement. After discontinuing the amplification assay, the plurality of reaction volumes may be heated and changes in the indicators of amplification of two or more of the at least some of the reaction volumes may be detected or measured.