Thomas A Gardner

US Patent:

20030213006, Nov 13, 2003

Filed:

Mar 31, 2003

Appl. No.:

10/296667

Inventors:

Ri-Hyun Back - Kobe, JP
Thomas Gardner - Zionsville IN, US
Akinobu Gotoli - Higashimada-ku, JP

International Classification:

A01K067/00
C07H021/04
C12P021/02
C12N005/06
C07K014/47
A61K031/522
A61K048/00
A61K031/513

US Classification:

800/008000, 536/023200, 424/093200, 514/044000, 530/350000, 435/069100, 435/320100, 435/325000, 514/263320, 514/269000

Abstract:

The present invention relates to promoters, enhancers and other regulatory elements that direct expression within tumor cells, comprising nucleotide sequences from the 5′ regulatory region, and transcriptionally active fragments thereof, that control expression of a testicular carcinoma related protein, -HCG. Specifically provided are expression vectors, host cells and transgenic animals wherein an -HCG regulatory region is capable of controlling expression of a heterologous gene, over-expressing an endogenous gene or an inhibitor of a pathological process or knocking out expression of a specific gene believed to be important in cancer development and/or progression. The invention also relates to methods for using said vectors, cells and animals for screening candidate molecules for agonists and antagonists of cancer development and/or progression. The invention further relates to compositions and methods for modulating expression of compounds within tumor cells, and to screening compounds that modulate expression within tumor cells. Methods for using the molecules and compounds identified by the screening assays for therapeutic treatments also are provided.

US Patent:

20040038232, Feb 26, 2004

Filed:

Mar 31, 2003

Appl. No.:

10/296666

Inventors:

Thomas Gardner - Zionsville IN, US
Chinghai Kao - Indianapolis IN, US
Fan Yeung - Charlottesville VA, US
Lelend Chung - Atlanta GA, US

International Classification:

C12Q001/68
C07H021/04
C07K014/705

US Classification:

435/006000, 435/069100, 435/320100, 435/325000, 530/350000, 536/023500

Abstract:

The present invention relates to promoters, enhancers and other regulatory elements that direct expression within tumor cells, comprising nucleotide sequences from the 5′ regulatory region, and transcriptionally active fragments thereof, that control expression of a renal cell carcinoma related protein, MN-CA9. Specifically provided are expression vectors, host cells and transgenic animals wherein an MN-CA9 regulatory region is capable of controlling expression of a heterologous gene, over-expressing an endogenous gene or an inhibitor of a pathological process or knocking out expression of a specific gene believed to be important in cancer development and/or progression. The invention also relates to methods for using said vectors, cells and animals for screening candidate molecules for agonists and antagonists of cancer development and/or progression. The invention further relates to compositions and methods for modulating expression of compounds within tumor cells, and to screening compounds that modulate expression within tumor cells. Methods for using the molecules and compounds identified by the screening assays for therapeutic treatments also are provided.

US Patent:

46713158, Jun 9, 1987

Filed:

Apr 29, 1986

Appl. No.:

6/857085

Inventors:

Thomas L. Gardner - Walnut Creek CA

International Classification:

F16L 300
B65H 7538

US Classification:

13735512

Abstract:

The present invention relates to brushfire fighting equipment and more particularly to a portable brushfire hydrant which can be easily stored and maintained in a condition for immediate use by an individual homeowner to protect his life and property from brushfire damage. In the past, individual home- owners could only utilize the commonly available garden hose which, of course, is grossly inadequate for the purpose or if a self-contained engine driven auxillary pump is available, it is usually not maintained or stored with all the accessory equipment necessary for the immediate and effective use in such fire fighting emergencies. The present invention successfully overcomes these deficiencies by providing a completely portable brushfire hydrant which has an open frame on which a high pressure pump can be securely mounted with all the accessories necessary conveniently disposed in a tightly compact storage condition on the frame for connection to and immediate use with the pump on a moment's notice.