Thomas J Greene

US Patent:

6900291, May 31, 2005

Filed:

Feb 25, 2003

Appl. No.:

10/375697

Inventors:

Eric H. Holmes - Bothell WA, US
Thomas G. Greene - Seattle WA, US

Assignee:

CytoChem, Inc. - Seattle WA

International Classification:

C12Q001/68

US Classification:

5303871, 530350, 530403, 5303881, 4241931, 435 6, 435 5, 435 72, 435 71, 435 791, 435 792

Abstract:

The present invention relates to methods and materials for the detection and quantitation 8-OH-Ade in biological specimens. Specifically, the present invention is directed to a group of highly specific monoclonal antibodies reactive with the modified nucleoside structure 8-OH-Ade, and to various immunoassays for 8-OH-Ade utilizing these monoclonal antibodies. The monoclonal antibodies of the present invention may be used in assays for diagnosing or monitoring the progression of certain types of cancer, in addition to a variety of other diseases associated with mutagenesis resulting from oxidative damage of DNA. Assays utilizing the monoclonal antibodies of the present invention may also be used to analyze or monitor toxicant exposure, such as from environmental sources. The monoclonal antibodies of the present invention were prepared with the immunogen 8-OH-adenosine coupled to keyhole limpet hemocyanin (KLH), not to 8-OH-Ade directly. It is believed that the monoclonal antibodies bind with the base portion of the structure (8-OH-Ade) and not the carbohydrate (ribose) or protein linkage region of the conjugate, because, as demonstrated, conjugates bound to nucleosides other than 8-OH-adenosine were unreactive with these antibodies.

US Patent:

61875511, Feb 13, 2001

Filed:

Mar 18, 1998

Appl. No.:

9/043461

Inventors:

Eric H. Holmes - Bothell WA
Thomas G. Greene - Seattle WA

Assignee:

CytoChem, Inc. - Seattle WA

International Classification:

G01N 3353

US Classification:

435 792

Abstract:

The present invention relates generally to assays for 8-hydroxyadenine (8-OH-Ade) and, more particularly, to improved immunoassays for the detection and quantitation of 8-OH-Ade in biological specimens. The present invention further relates to new monoclonal antibodies directed against 8-OH-Ade for use in the immunoassays.

Address:

12411 Riv Bnd, Austin, TX 78732

Phone:

5122661544

VIN:

2GCEK19B071120020

Make:

CHEVROLET

Model:

SILVERADO 1500 CLASSIC

Year:

2007

Address:

9217 Independence Loop, Austin, TX 78748

VIN:

1ZVHT85H775319512

Make:

FORD

Model:

MUSTANG

Year:

2007