Thomas S Myers

US Patent:

6346379, Feb 12, 2002

Filed:

Sep 3, 1998

Appl. No.:

09/146631

Inventors:

David Harrow Gelfand - Oakland CA
Lisa Vivian Kalman - San Francisco CA
Fred Lawrence Reichert - Oakland CA
Christopher Lim Sigua - Antioch CA
Thomas W. Myers - Alameda CA

Assignee:

F. Hoffman-La Roche AG - Basel

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 530300, 530327, 536 231, 536 232

Abstract:

Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.

US Patent:

7148049, Dec 12, 2006

Filed:

Mar 26, 2003

Appl. No.:

10/401403

Inventors:

Nancy J. Schoenbrunner - Moraga CA, US
Thomas W. Myers - Alameda CA, US
David H. Gelfand - Oakland CA, US

Assignee:

Roche Molecular Systems, Inc. - Alameda CA

International Classification:

C12N 9/12

US Classification:

435194, 435196

Abstract:

The present invention provides thermostable or thermoactive DNA polymerases with attenuated 3′-5′ exonuclease activity, methods for their synthesis, methods for their use, kits comprising the polymerases, nucleic acids encoding the polymerases and cells comprising such a nucleic acid. The DNA polymerases of the invention are useful in many recombinant DNA techniques, such as nucleic acid amplification by the polymerase chain reaction. The DNA polymerases of the invention allow higher fidelity replication and amplification of a template DNA sequence, allow less degradation of primers and/or more efficient use of deoxynucleotide triphosphates and are in general more efficient and less costly to make and use.

US Patent:

7179590, Feb 20, 2007

Filed:

Mar 30, 2001

Appl. No.:

09/823649

Inventors:

Edward Soh Smith - San Francisco CA, US
Carita Maria Elfstrom - San Francisco CA, US
David Harrow Gelfand - Oakland CA, US
Russell Gene Higuchi - Alameda CA, US
Thomas William Myers - Alameda CA, US
Alice Ming Wang - Lafayette CA, US

Assignee:

Roche Molecular Systems, Inc - Alameda CA

International Classification:

C07H 21/04
C12Q 1/68

US Classification:

435 6, 435 911, 435 912, 435 9121, 536 231

Abstract:

The present invention relates to improved reverse transcription methods using a modified thermostable DNA polymerases, particularly in a magnesium ion buffer. These methods are particularly useful in combined reverse-transcription/amplification reactions.

US Patent:

7282060, Oct 16, 2007

Filed:

Dec 21, 2004

Appl. No.:

11/020648

Inventors:

Leonard C DeBenedictis - Palo Alto CA, US
Thomas R Myers - Palo Alto CA, US
Kin F Chan - San Jose CA, US
George Frangineas, Jr. - Fremont CA, US

Assignee:

Reliant Technologies, Inc. - Mountain View CA

International Classification:

A61N 5/06

US Classification:

607 88, 128898, 606 9

Abstract:

The present invention provides methods and apparatus for controlling light-induced tissue treatment. In accordance with various aspects of the present invention, the invention provides for improved, real-time control of the light beam operational parameters which enables greater safety, efficiency, uniformity and continuity of the treatment process.

US Patent:

7546451, Jun 9, 2009

Filed:

Jun 19, 2002

Appl. No.:

10/174621

Inventors:

Chris Cicchetti - Menlo Park CA, US
Jean-François Dubé - Santa Clara CA, US
Thomas Andrew Myers - San Jose CA, US
An Huynh - Alameda CA, US
Geoffrey T. Hibbert - San Jose CA, US

Assignee:

Finisar Corporation - Sunnyvale CA

International Classification:

G06F 9/00

US Classification:

713100, 713 1, 713 2, 711 2, 711 5, 712206

Abstract:

A system and method for enabling a programmable device to execute instructions without interruption. An instruction space for storing instructions from a host application is bifurcated to define a program segment and a hold segment. At startup, instructions are loaded into the hold segment, and the programmable device begins executing those instructions. While the hold segment instructions are executed, the program segment is loaded with instructions. Once the program segment is filled, control is shifted to it and instructions from this segment are executed by the programmable device. When the program segment has been executed, control is shifted back to the hold segment, and instructions are taken from it while the program segment is reloaded with a fresh set of instructions from the host application. Once the program segment is reloaded, control is redirected and execution of instructions from the program segment is continued.

US Patent:

7572581, Aug 11, 2009

Filed:

Jun 28, 2004

Appl. No.:

10/879493

Inventors:

David Harrow Gelfand - Oakland CA, US
Fred Lawrence Reichert - San Leandro CA, US
Veeraiah Bodepudi - San Ramon CA, US
Amar Gupta - Danville CA, US
Stephen Will - Oakland CA, US
Thomas Myers - Alameda CA, US

Assignee:

Roche Molecular Systems, Inc. - Pleasanton CA

International Classification:

C12Q 1/68
C07H 19/00

US Classification:

435 6, 435975, 536 221

Abstract:

The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.

US Patent:

7598032, Oct 6, 2009

Filed:

Jan 30, 2003

Appl. No.:

10/355532

Inventors:

David Harrow Gelfand - Oakland CA, US
Lisa Vivian Kalman - San Francisco CA, US
Fred Lawrence Reichert - Oakland CA, US
Christopher Lim Sigua - Antioch CA, US
Thomas W. Myers - Alameda CA, US

Assignee:

Roche Molecular Systems - Pleasanton CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04

US Classification:

435 6, 435 912

Abstract:

Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.

US Patent:

7919249, Apr 5, 2011

Filed:

Jul 16, 2008

Appl. No.:

12/174488

Inventors:

David Harrow Gelfand - Oakland CA, US
Fred Lawrence Reichert - San Leandro CA, US
Veeraiah Bodepudi - San Ramon CA, US
Amar Gupta - Danville CA, US
Stephen Will - Oakland CA, US
Thomas Myers - Alameda CA, US

Assignee:

Roche Molecular Systems, Inc. - Pleasanton CA

International Classification:

C12Q 1/68
C07H 19/00

US Classification:

435 6, 435975, 536 221

Abstract:

The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2′-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.