Thuan N Trinh

US Patent:

20020064837, May 30, 2002

Filed:

May 7, 1999

Appl. No.:

09/306986

Inventors:

THUAN QUOC TRINH - GAITHERSBURG MD, US
CHRISTIAN ELLIOTT GRUBER - FREDERICK MD, US

International Classification:

C12Q001/68

US Classification:

435/091100, 435/006000, 435/091200

Abstract:

The subject invention provides novel compositions containing one or more peptides or polypeptides which possess ribonuclease activity. Preferably the ribonucleases for inclusion in the compositions are ribonucleases that substantially lack deoxyribonuclease activity. A preferred embodiment is a composition comprising RNase A or RNase T1. Another aspect of the invention is to provide methods for synthesizing nucleic acids, typically DNA, using the compositions of the invention or one or more ribonucleases. Another aspect of the invention involves the use of the subject method of nucleic acid synthesis to carry out the synthesis step in a polymerase chain reaction experiment. Yet another aspect of the invention is to provide kits for the synthesis of nucleic acids, wherein the kits comprise one or more peptides or polypeptides that possess ribonuclease activity.

US Patent:

20020127587, Sep 12, 2002

Filed:

Feb 13, 2002

Appl. No.:

10/073260

Inventors:

Domenica Simms - Silver Spring MD, US
Thuan Trinh - Gaithersburg MD, US

International Classification:

C12Q001/68
C12N001/08

US Classification:

435/006000, 435/270000

Abstract:

The present invention relates generally to compositions, methods and kits for use in clarification and viscosity reduction of biological samples. More specifically, the invention relates to such compositions, methods and kits that are useful in the isolation of biological macromolecules from cells (e.g., bacterial cells, animal cells, fungal cells, viruses, yeast cells or plant cells) via lysis and one or more additional isolation procedures, such as one or more filtration procedures. In particular, the invention relates to compositions, methods and kits wherein biological macromolecules are isolated using a filter, where the pore size increases in the direction of sample flow. The compositions, methods and kits of the invention are suitable for isolating a variety of forms of biological macromolecules from cells. The compositions, methods and kits of the invention are particularly well-suited for rapid isolation of nucleic acid molecules from bacterial cells.