Timothy R Coleman

US Patent:

20140024812, Jan 23, 2014

Filed:

Aug 27, 2013

Appl. No.:

14/011231

Inventors:

Shawn LAWRENCE - Valley Cottage NY, US
Timothy A. COLEMAN - Derwood MD, US

Assignee:

THE UNIVERSITY OF WYOMING - Lamarie WY
HUMAN GENOME SCIENCES, INC. - Rockville MD
THE JOHN HOPKINS UNIVERSITY - Baltimore MD

International Classification:

C12P 21/00

US Classification:

530395, 435 691

Abstract:

Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.

Address:

41 Jones Park Dr, Riverside, CT 06878

Phone:

2036373768

VIN:

WBANN73547CN04418

Make:

BMW

Model:

5 SERIES

Year:

2007